AEP1_YEAST
ID AEP1_YEAST Reviewed; 518 AA.
AC P32493; D6VZN8; P32446;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=ATPase expression protein 1, mitochondrial;
DE AltName: Full=Nuclear control of ATPase messenger RNA expression protein 1;
DE Flags: Precursor;
GN Name=AEP1; Synonyms=NCA1; OrderedLocusNames=YMR064W; ORFNames=YM9916.03;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=8358819; DOI=10.1007/bf00324676;
RA Payne M.J., Lukins H.B.;
RT "Characterization of a second nuclear gene, AEP1, required for expression
RT of the mitochondrial OLI1 gene in Saccharomyces cerevisiae.";
RL Curr. Genet. 24:126-135(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-500, AND FUNCTION.
RC STRAIN=SC167;
RX PubMed=8445655; DOI=10.1006/jmbi.1993.1095;
RA Ziaja K., Michaelis G., Lisowsky T.;
RT "Nuclear control of the messenger RNA expression for mitochondrial ATPase
RT subunit 9 in a new yeast mutant.";
RL J. Mol. Biol. 229:909-916(1993).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for translation of the mitochondrial OLI1 transcript
CC encoding subunit 9 of mitochondrial ATP synthase.
CC {ECO:0000269|PubMed:8358819, ECO:0000269|PubMed:8445655}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 504 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the AEP1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA78171.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; M80615; AAA02908.1; -; Genomic_DNA.
DR EMBL; X57087; CAA40367.1; -; Genomic_DNA.
DR EMBL; Z48952; CAA88789.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09962.1; -; Genomic_DNA.
DR EMBL; Z12301; CAA78171.1; ALT_FRAME; Genomic_DNA.
DR PIR; S52824; S52824.
DR RefSeq; NP_013780.1; NM_001182562.1.
DR AlphaFoldDB; P32493; -.
DR BioGRID; 35239; 74.
DR DIP; DIP-5193N; -.
DR IntAct; P32493; 4.
DR MINT; P32493; -.
DR STRING; 4932.YMR064W; -.
DR MaxQB; P32493; -.
DR PaxDb; P32493; -.
DR PRIDE; P32493; -.
DR EnsemblFungi; YMR064W_mRNA; YMR064W; YMR064W.
DR GeneID; 855086; -.
DR KEGG; sce:YMR064W; -.
DR SGD; S000004668; AEP1.
DR VEuPathDB; FungiDB:YMR064W; -.
DR eggNOG; ENOG502RXUX; Eukaryota.
DR HOGENOM; CLU_035453_0_0_1; -.
DR InParanoid; P32493; -.
DR OMA; CKVEANE; -.
DR BioCyc; YEAST:G3O-32766-MON; -.
DR PRO; PR:P32493; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P32493; protein.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0045182; F:translation regulator activity; IMP:SGD.
DR GO; GO:0006417; P:regulation of translation; IMP:SGD.
DR InterPro; IPR031467; Aep1.
DR Pfam; PF17049; AEP1; 1.
PE 1: Evidence at protein level;
KW Mitochondrion; Reference proteome; Transit peptide; Translation regulation.
FT TRANSIT 1..19
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 20..518
FT /note="ATPase expression protein 1, mitochondrial"
FT /id="PRO_0000021791"
FT CONFLICT 51
FT /note="E -> G (in Ref. 1; AAA02908 and 4; CAA78171)"
FT /evidence="ECO:0000305"
FT CONFLICT 113
FT /note="K -> R (in Ref. 1; AAA02908 and 4; CAA78171)"
FT /evidence="ECO:0000305"
FT CONFLICT 159
FT /note="I -> V (in Ref. 1; AAA02908 and 4; CAA78171)"
FT /evidence="ECO:0000305"
FT CONFLICT 197
FT /note="N -> D (in Ref. 1; AAA02908 and 4; CAA78171)"
FT /evidence="ECO:0000305"
FT CONFLICT 212
FT /note="K -> N (in Ref. 1; AAA02908 and 4; CAA78171)"
FT /evidence="ECO:0000305"
FT CONFLICT 225
FT /note="Y -> F (in Ref. 1; AAA02908 and 4; CAA78171)"
FT /evidence="ECO:0000305"
FT CONFLICT 240
FT /note="N -> H (in Ref. 1; AAA02908 and 4; CAA78171)"
FT /evidence="ECO:0000305"
FT CONFLICT 295
FT /note="K -> I (in Ref. 1; AAA02908 and 4; CAA78171)"
FT /evidence="ECO:0000305"
FT CONFLICT 392
FT /note="R -> Q (in Ref. 1; AAA02908 and 4; CAA78171)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 518 AA; 59748 MW; DE1F10728D288C9B CRC64;
MITTVQEISK WRNLCFIRMQ SRKWYPVLKK TPLVADGRKI IKHADKVPHP EEIIHPFYQP
TAIEQFTACA TEYNPSLLDG KKIAPSLIKH PVSLKTILVD SKLKFDDIRG VNKWLMEFVA
RRQHQRNIVL TPASKSVRSF HVLHLSSTDI AKLRGLENIL SEIENTNDLQ SRVESVNNEL
QNIFDRDSKQ TRLFCENILA YLIKNYGNST EKLILLINVT EMQLYSRLDQ MKAMNIILYN
ILCKVEANEN PPYSPTLVTA LENLLAAINN RFFPGRCENS LHPIVIEQLL SYFIKTGNLN
ESKNFLGHLI KKGILPEATI INRYLEAIDV HFDKSTKIFD IRSKFAFIAD LAPIIENYGT
IDLFKFLIPM CRHFDELCSL LNIIRKSNNA KRAVDSTLPI FIKKVLTFTK DPMINSGNLS
TVFNIVSPIY GQNVPSEFVE KFILSFALQG NYTMMAHMID TYKIKLSHKY QLQIIRALKN
SERNHALKNT GAVGYNKEFK KYFIEKYLNC TEREALRP
