3L22_DENVI
ID 3L22_DENVI Reviewed; 73 AA.
AC P01395;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Alpha-elapitoxin-Dv2a;
DE Short=Alpha-EPTX-Dv2a;
DE AltName: Full=Long neurotoxin 2;
DE AltName: Full=Toxin I/V;
OS Dendroaspis viridis (Western green mamba).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Elapidae; Elapinae; Dendroaspis.
OX NCBI_TaxID=8621;
RN [1]
RP PROTEIN SEQUENCE, TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RX PubMed=976267; DOI=10.1111/j.1432-1033.1976.tb10832.x;
RA Bechis G., Granier C., van Rietschoten J., Jover E., Rochat H., Miranda F.;
RT "Purification of six neurotoxins from the venom of Dendroaspis viridis.
RT Primary structure of two long toxins.";
RL Eur. J. Biochem. 68:445-456(1976).
CC -!- FUNCTION: Binds with high affinity to muscular (alpha-1/CHRNA1) and
CC neuronal (alpha-7/CHRNA7) nicotinic acetylcholine receptor (nAChR) and
CC inhibits acetylcholine from binding to the receptor, thereby impairing
CC neuromuscular and neuronal transmission.
CC {ECO:0000250|UniProtKB:P60615}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:976267}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC -!- TOXIC DOSE: LD(50) is 0.045 mg/kg by subcutaneous injection for variant
CC I, and 0.08 mg/kg for variant V. {ECO:0000269|PubMed:976267}.
CC -!- MISCELLANEOUS: The variant I sequence is shown.
CC -!- SIMILARITY: Belongs to the snake three-finger toxin family. Long-chain
CC subfamily. Type II alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR PIR; A01666; N2EP2V.
DR AlphaFoldDB; P01395; -.
DR SMR; P01395; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR CDD; cd00206; snake_toxin; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR003571; Snake_3FTx.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR018354; Snake_toxin_con_site.
DR InterPro; IPR035076; Toxin/TOLIP.
DR Pfam; PF00087; Toxin_TOLIP; 1.
DR SUPFAM; SSF57302; SSF57302; 1.
DR PROSITE; PS00272; SNAKE_TOXIN; 1.
PE 1: Evidence at protein level;
KW Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW Postsynaptic neurotoxin; Secreted; Toxin.
FT CHAIN 1..73
FT /note="Alpha-elapitoxin-Dv2a"
FT /id="PRO_0000093542"
FT DISULFID 3..21
FT /evidence="ECO:0000250"
FT DISULFID 14..42
FT /evidence="ECO:0000250"
FT DISULFID 27..31
FT /evidence="ECO:0000250"
FT DISULFID 46..57
FT /evidence="ECO:0000250"
FT DISULFID 58..63
FT /evidence="ECO:0000250"
FT VARIANT 73
FT /note="Missing (in variant V)"
SQ SEQUENCE 73 AA; 8054 MW; 0D7A3BC25B46C63B CRC64;
RTCYKTPSVK PETCPHGENI CYTETWCDAW CSQRGKRVEL GCAATCPKVK AGVGIKCCST
DNCNPFPVWN PRG