AEP2_YEAST
ID AEP2_YEAST Reviewed; 580 AA.
AC P22136; D6W0A9; P23115;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 3.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=ATPase expression protein 2, mitochondrial;
DE Flags: Precursor;
GN Name=AEP2; Synonyms=ATP13; OrderedLocusNames=YMR282C; ORFNames=YM8021.08C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=1825065; DOI=10.1016/0014-5793(91)80124-l;
RA Ackerman S.H., Gatti D.L., Gellefors P., Douglas M.G., Tzagoloff A.;
RT "ATP13, a nuclear gene of Saccharomyces cerevisiae essential for the
RT expression of subunit 9 of the mitochondrial ATPase.";
RL FEBS Lett. 278:234-238(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=1718609; DOI=10.1007/bf00312765;
RA Payne M.J., Finnegan P.M., Keramidaris E., Lukins H.B.;
RT "Characterization of a yeast nuclear gene, AEP2, required for accumulation
RT of mitochondrial mRNA encoding subunit 9 of the ATP synthase.";
RL Curr. Genet. 20:53-61(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-108.
RC STRAIN=S288c / YPH1;
RX PubMed=7954819; DOI=10.1016/0092-8674(94)90262-3;
RA Aastroem S.U., Bystroem A.S.;
RT "Rit1, a tRNA backbone-modifying enzyme that mediates initiator and
RT elongator tRNA discrimination.";
RL Cell 79:535-546(1994).
RN [6]
RP FUNCTION, MRNA-BINDING, AND MUTAGENESIS OF LEU-413.
RX PubMed=10101162; DOI=10.1093/genetics/151.4.1353;
RA Ellis T.P., Lukins H.B., Nagley P., Corner B.E.;
RT "Suppression of a nuclear aep2 mutation in Saccharomyces cerevisiae by a
RT base substitution in the 5'-untranslated region of the mitochondrial oli1
RT gene encoding subunit 9 of ATP synthase.";
RL Genetics 151:1353-1363(1999).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for translation of the mitochondrial OLI1 transcript
CC coding for the mitochondrial ATP synthase subunit 9.
CC {ECO:0000269|PubMed:10101162, ECO:0000269|PubMed:1718609,
CC ECO:0000269|PubMed:1825065}.
CC -!- SUBUNIT: Binds to the 5'UTR of the OLI1 mRNA.
CC -!- SUBCELLULAR LOCATION: Mitochondrion.
CC -!- MISCELLANEOUS: Present with 2680 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the AEP2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA39672.2; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X56215; CAA39672.2; ALT_FRAME; Genomic_DNA.
DR EMBL; M59860; AAA34412.1; -; Genomic_DNA.
DR EMBL; Z49704; CAA89780.1; -; Genomic_DNA.
DR EMBL; X80795; CAA56771.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10183.1; -; Genomic_DNA.
DR PIR; S54589; S54589.
DR RefSeq; NP_014009.1; NM_001182789.1.
DR AlphaFoldDB; P22136; -.
DR BioGRID; 35461; 260.
DR DIP; DIP-6693N; -.
DR IntAct; P22136; 2.
DR STRING; 4932.YMR282C; -.
DR iPTMnet; P22136; -.
DR MaxQB; P22136; -.
DR PaxDb; P22136; -.
DR PRIDE; P22136; -.
DR EnsemblFungi; YMR282C_mRNA; YMR282C; YMR282C.
DR GeneID; 855325; -.
DR KEGG; sce:YMR282C; -.
DR SGD; S000004895; AEP2.
DR VEuPathDB; FungiDB:YMR282C; -.
DR eggNOG; ENOG502RX9I; Eukaryota.
DR HOGENOM; CLU_035070_0_0_1; -.
DR InParanoid; P22136; -.
DR OMA; LQLRCGA; -.
DR BioCyc; YEAST:G3O-32952-MON; -.
DR PRO; PR:P22136; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P22136; protein.
DR GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0070124; P:mitochondrial translational initiation; IGI:SGD.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR InterPro; IPR024319; ATPase_expression_mit.
DR Pfam; PF12921; ATP13; 1.
PE 1: Evidence at protein level;
KW Mitochondrion; Reference proteome; RNA-binding; Transit peptide;
KW Translation regulation.
FT TRANSIT 1..40
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 41..580
FT /note="ATPase expression protein 2, mitochondrial"
FT /id="PRO_0000002536"
FT MUTAGEN 413
FT /note="L->P: Suppresses a T-to-C nucleotide transition
FT mutation in the 5' untranslated region of OLI1 mRNA
FT localized 16 nucleotides upstream of the OLI1 reading
FT frame."
FT /evidence="ECO:0000269|PubMed:10101162"
FT CONFLICT 214
FT /note="G -> N (in Ref. 1; CAA39672)"
FT /evidence="ECO:0000305"
FT CONFLICT 481
FT /note="I -> V (in Ref. 1; AAA34412)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 580 AA; 67523 MW; BC0AB10EABCDA4AA CRC64;
MWINRLVKHP SYSVLRFYTK RLCTVSVKSL REFGVLPNST ICHSVYPRRT YVMGRAVIND
ILIKKSYSTH TVCAIDRSKD ENNGSAYDKF EAKGIPIDVH TLKRIISSSG MDESEFSKSI
SYLFAKTVDP EPKDVLSLED LSFLLSKLYT QRFQIRRICR DINAKYSEFW FKLFSLYAEK
VDAKRNQVNL RNTKLDACEI FDANLMIKNF IELGQLGKAQ KILSFILDRN PDILLSPKNA
DISTIVHFLQ LRCGALAPYW KIPDNSEQKQ GFLRKMVRLG AKNTSIRLSS TYKAMDHQTL
LKIADLALQE KKLLNSEDLL STLIQSFGHL GQTQILERCI EHIWQISPQE FPSHVVIKHR
GCYPSSKILV SILVSFYFND HDLHRGLSIL DSFIKHYPDV KLDALFWRRL FQLSHFAWTP
ANDKKATSVV RCWHLMKQWY ASKRLRPSVD YETLRQLYDI MKKTGNFPLG IDVLRSFKPG
IERTRAENAG KVNNIIIKYQ KCIIKELVNR GRFSAVREFI DSYGFDRKMT KDLNIFCANR
MFLRSKKMKN KIENKKEREK VRLDSFDDDE DDGMIIGSLW
