AEP3_YEAST
ID AEP3_YEAST Reviewed; 606 AA.
AC Q12089; D6W407;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=ATPase expression protein 3;
GN Name=AEP3; OrderedLocusNames=YPL005W; ORFNames=YP8132.08;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=14742425; DOI=10.1074/jbc.m314162200;
RA Ellis T.P., Helfenbein K.G., Tzagoloff A., Dieckmann C.L.;
RT "Aep3p stabilizes the mitochondrial bicistronic mRNA encoding subunits 6
RT and 8 of the H+-translocating ATP synthase of Saccharomyces cerevisiae.";
RL J. Biol. Chem. 279:15728-15733(2004).
CC -!- FUNCTION: Required for respiration. Stabilizes the mitochondrial
CC bicistronic mRNA encoding ATP6 and ATP8, 2 subunits of the proton-
CC translocating ATP synthase. {ECO:0000269|PubMed:14742425}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:14742425}; Peripheral
CC membrane protein {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:14742425}; Matrix side {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:14742425}.
CC -!- MISCELLANEOUS: Present with 1760 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z48483; CAA88381.1; -; Genomic_DNA.
DR EMBL; Z71255; CAA95036.1; -; Genomic_DNA.
DR EMBL; U33335; AAB68100.1; -; Genomic_DNA.
DR EMBL; AY692659; AAT92678.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11423.1; -; Genomic_DNA.
DR PIR; S52526; S52526.
DR RefSeq; NP_015320.1; NM_001183819.1.
DR AlphaFoldDB; Q12089; -.
DR SMR; Q12089; -.
DR BioGRID; 36172; 79.
DR DIP; DIP-4045N; -.
DR MINT; Q12089; -.
DR STRING; 4932.YPL005W; -.
DR MaxQB; Q12089; -.
DR PaxDb; Q12089; -.
DR PRIDE; Q12089; -.
DR EnsemblFungi; YPL005W_mRNA; YPL005W; YPL005W.
DR GeneID; 856102; -.
DR KEGG; sce:YPL005W; -.
DR SGD; S000005926; AEP3.
DR VEuPathDB; FungiDB:YPL005W; -.
DR eggNOG; ENOG502QVB0; Eukaryota.
DR HOGENOM; CLU_457135_0_0_1; -.
DR InParanoid; Q12089; -.
DR OMA; TWTTCYN; -.
DR BioCyc; YEAST:G3O-33924-MON; -.
DR PRO; PR:Q12089; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q12089; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0019898; C:extrinsic component of membrane; IDA:SGD.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0070124; P:mitochondrial translational initiation; IGI:SGD.
DR GO; GO:0016071; P:mRNA metabolic process; IMP:SGD.
DR Gene3D; 1.25.40.10; -; 2.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF13812; PPR_3; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat.
FT CHAIN 1..606
FT /note="ATPase expression protein 3"
FT /id="PRO_0000064466"
FT REPEAT 220..254
FT /note="PPR 1"
FT REPEAT 397..432
FT /note="PPR 2"
FT REPEAT 433..467
FT /note="PPR 3"
SQ SEQUENCE 606 AA; 70310 MW; B7F93D1FD22677F4 CRC64;
MNTLRCLTQA LSKSGREAPK LYQKVIFPGL FREGIPIANV KKVDEKIIDS PTSTSVNGEA
KKIVRHGVKY EREQVKEYLS SLPTLTLSRK QIRDDYDEER AKRMYMFSKQ TNSSNKFQKL
LTAKSQEFTR ELLTLLIDCT SNEKNSGPER FTRKFLKFSN DEIPPLPDFS KNPQLFENYI
GILSHTKFNF RSSSKLNGIV RKMLRHLLHP TNKTTLPLRS AQVYNDSIYF FSEHFDFASC
REIFAQMKAE GTKPNTITFN LLLRNVVKNS HIRKTKHPDD EVLFYLRSMR NHGVFADVIT
WTTCYNFLRD EVSRQLYIVQ MGEHLGNFNV NFVYTVLRNG DYRAEDCLKV LAANSLPISR
KTFYLCIERL LNEEQLETAS KLLDYGFQHL KSNFKLDSEA MNHFMRVFAN KGRSDLAFLC
YNTCRKIYKI KPDSQTFEML FKALVRNGNT KNFGAVLQYI KDLKVSEGFG LRTSYWRTKA
DSIFKFGSPN TLSEKSIEKA RKLLGNLIAS EGEFSWKIWK ESDSSQKKIL RFLGCIPTTL
RCTNTAQDHQ KPTNLPSNIS QKKREYRNRV KAIATKAALE KRMAYIKDND VAFKKELVKR
RIVGEV
