AEPE_BPA50
ID AEPE_BPA50 Reviewed; 289 AA.
AC Q37979;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=L-alanyl-D-glutamate peptidase;
DE EC=3.4.24.-;
GN Name=ply; Synonyms=ply500;
OS Listeria phage A500 (Bacteriophage A500).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Siphoviridae.
OX NCBI_TaxID=40522;
OH NCBI_TaxID=1639; Listeria monocytogenes.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8577256; DOI=10.1111/j.1365-2958.1995.tb02345.x;
RA Loessner M.J., Wendlinger G., Scherer S.;
RT "Heterogeneous endolysins in Listeria monocytogenes bacteriophages: a new
RT class of enzymes and evidence for conserved holin genes within the
RT siphoviral lysis cassettes.";
RL Mol. Microbiol. 16:1231-1241(1995).
CC -!- FUNCTION: Cell wall lytic enzyme. Hydrolyzes the link between L-alanine
CC and D-glutamate residues in certain bacterial cell-wall glycopeptides.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- DEVELOPMENTAL STAGE: Expressed at about 20 minutes after infection.
CC -!- SIMILARITY: Belongs to the peptidase M15C family. {ECO:0000305}.
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DR EMBL; X85009; CAA59365.1; -; Genomic_DNA.
DR PIR; S69801; S69801.
DR RefSeq; YP_001468411.1; NC_009810.1.
DR PDB; 2VO9; X-ray; 1.80 A; A/B/C=1-167.
DR PDB; 6HX0; X-ray; 1.59 A; A=133-289.
DR PDBsum; 2VO9; -.
DR PDBsum; 6HX0; -.
DR SMR; Q37979; -.
DR MEROPS; M15.021; -.
DR GeneID; 5601386; -.
DR KEGG; vg:5601386; -.
DR EvolutionaryTrace; Q37979; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008233; F:peptidase activity; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR Gene3D; 3.30.1380.10; -; 1.
DR InterPro; IPR009045; Hedgehog_sig/DD-Pept_Zn-bd_sf.
DR InterPro; IPR039561; Peptidase_M15C.
DR InterPro; IPR041341; PSA_CBD.
DR Pfam; PF13539; Peptidase_M15_4; 1.
DR Pfam; PF18341; PSA_CBD; 2.
DR SUPFAM; SSF55166; SSF55166; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell wall biogenesis/degradation; Hydrolase; Secreted.
FT CHAIN 1..289
FT /note="L-alanyl-D-glutamate peptidase"
FT /id="PRO_0000217836"
FT HELIX 5..16
FT /evidence="ECO:0007829|PDB:2VO9"
FT HELIX 23..37
FT /evidence="ECO:0007829|PDB:2VO9"
FT TURN 38..40
FT /evidence="ECO:0007829|PDB:2VO9"
FT STRAND 43..47
FT /evidence="ECO:0007829|PDB:2VO9"
FT HELIX 52..60
FT /evidence="ECO:0007829|PDB:2VO9"
FT TURN 61..63
FT /evidence="ECO:0007829|PDB:2VO9"
FT STRAND 64..67
FT /evidence="ECO:0007829|PDB:2VO9"
FT HELIX 79..82
FT /evidence="ECO:0007829|PDB:2VO9"
FT STRAND 85..91
FT /evidence="ECO:0007829|PDB:2VO9"
FT STRAND 95..99
FT /evidence="ECO:0007829|PDB:2VO9"
FT STRAND 102..104
FT /evidence="ECO:0007829|PDB:2VO9"
FT HELIX 105..116
FT /evidence="ECO:0007829|PDB:2VO9"
FT HELIX 122..124
FT /evidence="ECO:0007829|PDB:2VO9"
FT STRAND 125..128
FT /evidence="ECO:0007829|PDB:2VO9"
FT STRAND 133..137
FT /evidence="ECO:0007829|PDB:2VO9"
FT HELIX 139..141
FT /evidence="ECO:0007829|PDB:2VO9"
FT STRAND 158..163
FT /evidence="ECO:0007829|PDB:6HX0"
FT STRAND 165..173
FT /evidence="ECO:0007829|PDB:6HX0"
FT STRAND 180..184
FT /evidence="ECO:0007829|PDB:6HX0"
FT STRAND 188..193
FT /evidence="ECO:0007829|PDB:6HX0"
FT STRAND 195..203
FT /evidence="ECO:0007829|PDB:6HX0"
FT STRAND 206..211
FT /evidence="ECO:0007829|PDB:6HX0"
FT HELIX 212..214
FT /evidence="ECO:0007829|PDB:6HX0"
FT STRAND 215..217
FT /evidence="ECO:0007829|PDB:6HX0"
FT STRAND 227..231
FT /evidence="ECO:0007829|PDB:6HX0"
FT TURN 234..236
FT /evidence="ECO:0007829|PDB:6HX0"
FT STRAND 239..244
FT /evidence="ECO:0007829|PDB:6HX0"
FT STRAND 248..250
FT /evidence="ECO:0007829|PDB:6HX0"
FT STRAND 252..255
FT /evidence="ECO:0007829|PDB:6HX0"
FT STRAND 260..264
FT /evidence="ECO:0007829|PDB:6HX0"
FT STRAND 267..275
FT /evidence="ECO:0007829|PDB:6HX0"
FT TURN 276..278
FT /evidence="ECO:0007829|PDB:6HX0"
FT STRAND 279..283
FT /evidence="ECO:0007829|PDB:6HX0"
FT TURN 285..287
FT /evidence="ECO:0007829|PDB:6HX0"
SQ SEQUENCE 289 AA; 33424 MW; 1CD6B52B37260A6A CRC64;
MALTEAWLIE KANRKLNAGG MYKITSDKTR NVIKKMAKEG IYLCVAQGYR STAEQNALYA
QGRTKPGAIV TNAKGGQSNH NYGVAVDLCL YTNDGKDVIW ESTTSRWKKV VAAMKAEGFK
WGGDWKSFKD YPHFELCDAV SGEKIPAATQ NTNTNSNRYE GKVIDSAPLL PKMDFKSSPF
RMYKVGTEFL VYDHNQYWYK TYIDDKLYYM YKSFCDVVAK KDAKGRIKVR IKSAKDLRIP
VWNNIKLNSG KIKWYAPNVK LAWYNYRRGY LELWYPNDGW YYTAEYFLK
