AER_ECOLI
ID AER_ECOLI Reviewed; 506 AA.
AC P50466; Q2M9D4;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Aerotaxis receptor;
GN Name=aer; Synonyms=air, yqjJ; OrderedLocusNames=b3072, JW3043;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP FUNCTION.
RX PubMed=9190831; DOI=10.1128/jb.179.12.4075-4079.1997;
RA Bibikov S.I., Biran R., Rudd K.E., Parkinson J.S.;
RT "A signal transducer for aerotaxis in Escherichia coli.";
RL J. Bacteriol. 179:4075-4079(1997).
RN [4]
RP FUNCTION.
RX PubMed=9380671; DOI=10.1073/pnas.94.20.10541;
RA Rebbapragada A., Johnson M.S., Harding G.P., Zuccarelli A.J.,
RA Fletcher H.M., Zhulin I.B., Taylor B.L.;
RT "The Aer protein and the serine chemoreceptor Tsr independently sense
RT intracellular energy levels and transduce oxygen, redox, and energy signals
RT for Escherichia coli behavior.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:10541-10546(1997).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [6]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=22380631; DOI=10.1111/j.1365-2958.2012.08021.x;
RA Li G., Young K.D.;
RT "Isolation and identification of new inner membrane-associated proteins
RT that localize to cell poles in Escherichia coli.";
RL Mol. Microbiol. 84:276-295(2012).
CC -!- FUNCTION: Signal transducer for aerotaxis. The aerotactic response is
CC the accumulation of cells around air bubbles. The nature of the sensory
CC stimulus detected by this protein is the proton motive force or
CC cellular redox state. It uses a FAD prosthetic group as a redox sensor
CC to monitor oxygen levels. {ECO:0000269|PubMed:9190831,
CC ECO:0000269|PubMed:9380671}.
CC -!- INTERACTION:
CC P50466; P50466: aer; NbExp=4; IntAct=EBI-1130981, EBI-1130981;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:22380631}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:22380631}. Note=Predominantly localized to one cell
CC pole in mid-to-late exponential phase, with a few smaller foci
CC elsewhere in the cell.
CC -!- SIMILARITY: Belongs to the methyl-accepting chemotaxis (MCP) protein
CC family. {ECO:0000305}.
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DR EMBL; U28379; AAA89151.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76107.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77122.1; -; Genomic_DNA.
DR PIR; E65095; E65095.
DR RefSeq; NP_417543.1; NC_000913.3.
DR RefSeq; WP_000094721.1; NZ_LN832404.1.
DR AlphaFoldDB; P50466; -.
DR SMR; P50466; -.
DR BioGRID; 4262399; 187.
DR DIP; DIP-9061N; -.
DR IntAct; P50466; 4.
DR STRING; 511145.b3072; -.
DR PaxDb; P50466; -.
DR PRIDE; P50466; -.
DR EnsemblBacteria; AAC76107; AAC76107; b3072.
DR EnsemblBacteria; BAE77122; BAE77122; BAE77122.
DR GeneID; 945301; -.
DR KEGG; ecj:JW3043; -.
DR KEGG; eco:b3072; -.
DR PATRIC; fig|1411691.4.peg.3658; -.
DR EchoBASE; EB2789; -.
DR eggNOG; COG0840; Bacteria.
DR HOGENOM; CLU_000445_107_26_6; -.
DR InParanoid; P50466; -.
DR OMA; PAMPLRW; -.
DR PhylomeDB; P50466; -.
DR BioCyc; EcoCyc:G7595-MON; -.
DR PRO; PR:P50466; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IMP:EcoCyc.
DR GO; GO:0006935; P:chemotaxis; IBA:GO_Central.
DR GO; GO:0052131; P:positive aerotaxis; IMP:EcoCyc.
DR GO; GO:0007165; P:signal transduction; IMP:EcoCyc.
DR CDD; cd00130; PAS; 1.
DR InterPro; IPR004090; Chemotax_Me-accpt_rcpt.
DR InterPro; IPR004089; MCPsignal_dom.
DR InterPro; IPR001610; PAC.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR013655; PAS_fold_3.
DR Pfam; PF00015; MCPsignal; 1.
DR Pfam; PF08447; PAS_3; 1.
DR PRINTS; PR00260; CHEMTRNSDUCR.
DR SMART; SM00283; MA; 1.
DR SMART; SM00086; PAC; 1.
DR SUPFAM; SSF55785; SSF55785; 1.
DR TIGRFAMs; TIGR00229; sensory_box; 1.
DR PROSITE; PS50111; CHEMOTAXIS_TRANSDUC_2; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Chemotaxis; FAD; Flavoprotein;
KW Membrane; Methylation; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..506
FT /note="Aerotaxis receptor"
FT /id="PRO_0000110565"
FT TOPO_DOM 1..166
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..190
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 210..506
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 263..492
FT /note="Methyl-accepting transducer"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00284"
SQ SEQUENCE 506 AA; 55066 MW; 913DEBCF14E3FD08 CRC64;
MSSHPYVTQQ NTPLADDTTL MSTTDLQSYI THANDTFVQV SGYTLQELQG QPHNMVRHPD
MPKAAFADMW FTLKKGEPWS GIVKNRRKNG DHYWVRANAV PMVREGKISG YMSIRTRATD
EEIAAVEPLY KALNAGRTSK RIHKGLVVRK GWLGKLPSLP LRWRARGVMT LMFILLAAML
WFVAAPVVTY ILCALVVLLA SACFEWQIVR PIENVAHQAL KVATGERNSV EHLNRSDELG
LTLRAVGQLG LMCRWLINDV SSQVSSVRNG SETLAKGTDE LNEHTQQTVD NVQQTVATMN
QMAASVKQNS ATASAADKLS ITASNAAVQG GEAMTTVIKT MDDIADSTQR IGTITSLIND
IAFQTNILAL NAAVEAARAG EQGKGFAVVA GEVRHLASRS ANAANDIRKL IDASADKVQS
GSQQVHAAGR TMEDIVAQVK NVTQLIAQIS HSTLEQADGL SSLTRAVDEL NLITQKNAEL
VEESAQVSAM VKHRASRLED AVTVLH
