AER_PSEAE
ID AER_PSEAE Reviewed; 521 AA.
AC Q9I3F6;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Methyl-accepting chemotaxis protein Aer {ECO:0000305};
DE AltName: Full=Aerotaxis receptor Aer {ECO:0000305};
GN Name=aer {ECO:0000303|PubMed:14987771};
GN Synonyms=tlpC {ECO:0000303|PubMed:14987771};
GN OrderedLocusNames=PA1561 {ECO:0000312|EMBL:AAG04950.1};
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP FUNCTION IN AEROTAXIS, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=14987771; DOI=10.1016/s0378-1097(04)00009-6;
RA Hong C.S., Shitashiro M., Kuroda A., Ikeda T., Takiguchi N., Ohtake H.,
RA Kato J.;
RT "Chemotaxis proteins and transducers for aerotaxis in Pseudomonas
RT aeruginosa.";
RL FEMS Microbiol. Lett. 231:247-252(2004).
RN [3]
RP INDUCTION.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=16233612; DOI=10.1016/s1389-1723(04)70188-7;
RA Hong C.S., Kuroda A., Ikeda T., Takiguchi N., Ohtake H., Kato J.;
RT "The aerotaxis transducer gene aer, but not aer-2, is transcriptionally
RT regulated by the anaerobic regulator ANR in Pseudomonas aeruginosa.";
RL J. Biosci. Bioeng. 97:184-190(2004).
CC -!- FUNCTION: Chemotactic transducer for aerotaxis.
CC {ECO:0000269|PubMed:14987771}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- INDUCTION: Expression is regulated by the transcriptional regulatory
CC protein of anaerobic gene expression, ANR. Both ANR box1 and box2 are
CC required for the functioning of the aer promoter.
CC {ECO:0000269|PubMed:16233612}.
CC -!- DISRUPTION PHENOTYPE: Mutant shows decreased aerotaxis. Aerotaxis is
CC abolished in the aer-mcpB double mutant. {ECO:0000269|PubMed:14987771}.
CC -!- SIMILARITY: Belongs to the methyl-accepting chemotaxis (MCP) protein
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE004091; AAG04950.1; -; Genomic_DNA.
DR PIR; A83451; A83451.
DR RefSeq; NP_250252.1; NC_002516.2.
DR RefSeq; WP_003087330.1; NZ_QZGE01000003.1.
DR AlphaFoldDB; Q9I3F6; -.
DR SMR; Q9I3F6; -.
DR STRING; 287.DR97_374; -.
DR PaxDb; Q9I3F6; -.
DR PRIDE; Q9I3F6; -.
DR EnsemblBacteria; AAG04950; AAG04950; PA1561.
DR GeneID; 879271; -.
DR KEGG; pae:PA1561; -.
DR PATRIC; fig|208964.12.peg.1617; -.
DR PseudoCAP; PA1561; -.
DR HOGENOM; CLU_000445_107_26_6; -.
DR InParanoid; Q9I3F6; -.
DR OMA; AVFEHMW; -.
DR PhylomeDB; Q9I3F6; -.
DR BioCyc; PAER208964:G1FZ6-1590-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR GO; GO:0009454; P:aerotaxis; IMP:PseudoCAP.
DR GO; GO:0052131; P:positive aerotaxis; IMP:CACAO.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR CDD; cd00130; PAS; 1.
DR InterPro; IPR004090; Chemotax_Me-accpt_rcpt.
DR InterPro; IPR004089; MCPsignal_dom.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR013655; PAS_fold_3.
DR InterPro; IPR000727; T_SNARE_dom.
DR Pfam; PF00015; MCPsignal; 1.
DR Pfam; PF08447; PAS_3; 1.
DR PRINTS; PR00260; CHEMTRNSDUCR.
DR SMART; SM00283; MA; 1.
DR SUPFAM; SSF55785; SSF55785; 1.
DR TIGRFAMs; TIGR00229; sensory_box; 1.
DR PROSITE; PS50111; CHEMOTAXIS_TRANSDUC_2; 1.
DR PROSITE; PS50112; PAS; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Chemotaxis; Membrane; Methylation;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..521
FT /note="Methyl-accepting chemotaxis protein Aer"
FT /id="PRO_0000454746"
FT TRANSMEM 147..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 21..76
FT /note="PAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 249..485
FT /note="Methyl-accepting transducer"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00284"
SQ SEQUENCE 521 AA; 57180 MW; 51795FA2434DC45D CRC64;
MRNNQPITQH ERVYPAEQRL ITTTNLKGII TYCNEAFIDI SGFSREELMS APHNLIRHPD
VPPAVFAHMW TTLKAGRPWM GIVKNRCKNG DHYWVSAYVT PIYDQGAVVG YESVRVKPTA
EQIQRAEALY RRLGAGKPAI PRRDRWLPVL LDWLPFILIS QIGFLIGIWL NSWWGFILAG
LLAVPLGLAG LRWQKRGLKR LMRLAEQTTS DPLIAQMYTD SRGDQARLEM AILSQDARLK
TCLTRLQDTA EYLTEQARQA DTLAHHSSAG LEQQRAETEQ VATAVNEMAA TTQEVANNVQ
LTADATQKAN ELTSRGRDIA AETRNAIQRL SESVGETGAA VSRLAQDSNE IGGVVDVIKG
IADQTNLLAL NAAIEAARAG DQGRGFAVVA DEVRSLAQRT AASTEQIHHL IAKLQNTAND
AVHTMESGLQ QAEAGVQRVL EADSALVGIS EAVSNITEMT TQIAAAAEEQ SAVAEEINRN
ISTIAALAEQ TSDEALRTAK LSEELTTTAQ SQYSLVERFN R
