EFR3B_MOUSE
ID EFR3B_MOUSE Reviewed; 817 AA.
AC Q6ZQ18; B2RXT1;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Protein EFR3 homolog B;
GN Name=Efr3b; Synonyms=Kiaa0953;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 117-817 (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-212; SER-214 AND SER-216, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=25380825; DOI=10.1242/jcs.157495;
RA Bojjireddy N., Guzman-Hernandez M.L., Reinhard N.R., Jovic M., Balla T.;
RT "EFR3s are palmitoylated plasma membrane proteins that control
RT responsiveness to G-protein-coupled receptors.";
RL J. Cell Sci. 128:118-128(2015).
CC -!- FUNCTION: Component of a complex required to localize
CC phosphatidylinositol 4-kinase (PI4K) to the plasma membrane. The
CC complex acts as a regulator of phosphatidylinositol 4-phosphate
CC (PtdIns(4)P) synthesis. In the complex, EFR3B probably acts as the
CC membrane-anchoring component. Also involved in responsiveness to G-
CC protein-coupled receptors; it is however unclear whether this role is
CC direct or indirect. {ECO:0000250|UniProtKB:Q9Y2G0}.
CC -!- SUBUNIT: Component of a phosphatidylinositol 4-kinase (PI4K) complex,
CC composed of PI4KA, EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and
CC FAM126 (FAM126A or FAM126B). {ECO:0000250|UniProtKB:Q9Y2G0}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9Y2G0};
CC Lipid-anchor {ECO:0000250|UniProtKB:Q9Y2G0}. Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:Q9Y2G0}. Note=Palmitoylation anchors the protein
CC to the plasma membrane. A small amount is observed in the cytosol.
CC {ECO:0000250|UniProtKB:Q9Y2G0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6ZQ18-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6ZQ18-2; Sequence=VSP_029808;
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:25380825}.
CC -!- PTM: Palmitoylated at its N-terminus, anchoring the protein to the
CC plasma membrane. {ECO:0000250|UniProtKB:Q9Y2G0}.
CC -!- SIMILARITY: Belongs to the EFR3 family. {ECO:0000305}.
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DR EMBL; AC111092; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC147643; AAI47644.1; -; mRNA.
DR EMBL; BC157968; AAI57969.1; -; mRNA.
DR EMBL; AK129247; BAC98057.1; -; mRNA.
DR CCDS; CCDS49016.1; -. [Q6ZQ18-1]
DR RefSeq; NP_001075952.1; NM_001082483.1. [Q6ZQ18-1]
DR AlphaFoldDB; Q6ZQ18; -.
DR BioGRID; 580010; 2.
DR IntAct; Q6ZQ18; 1.
DR MINT; Q6ZQ18; -.
DR STRING; 10090.ENSMUSP00000106809; -.
DR iPTMnet; Q6ZQ18; -.
DR PhosphoSitePlus; Q6ZQ18; -.
DR SwissPalm; Q6ZQ18; -.
DR jPOST; Q6ZQ18; -.
DR MaxQB; Q6ZQ18; -.
DR PaxDb; Q6ZQ18; -.
DR PeptideAtlas; Q6ZQ18; -.
DR PRIDE; Q6ZQ18; -.
DR ProteomicsDB; 275444; -. [Q6ZQ18-1]
DR ProteomicsDB; 275445; -. [Q6ZQ18-2]
DR Antibodypedia; 51573; 69 antibodies from 14 providers.
DR Ensembl; ENSMUST00000111178; ENSMUSP00000106809; ENSMUSG00000020658. [Q6ZQ18-1]
DR GeneID; 668212; -.
DR KEGG; mmu:668212; -.
DR UCSC; uc007mxh.1; mouse. [Q6ZQ18-1]
DR UCSC; uc011yjt.1; mouse. [Q6ZQ18-2]
DR CTD; 22979; -.
DR MGI; MGI:2444851; Efr3b.
DR VEuPathDB; HostDB:ENSMUSG00000020658; -.
DR eggNOG; KOG1877; Eukaryota.
DR GeneTree; ENSGT00390000002143; -.
DR HOGENOM; CLU_012674_1_0_1; -.
DR InParanoid; Q6ZQ18; -.
DR OMA; CQHIREV; -.
DR OrthoDB; 173880at2759; -.
DR PhylomeDB; Q6ZQ18; -.
DR TreeFam; TF314098; -.
DR BioGRID-ORCS; 668212; 3 hits in 72 CRISPR screens.
DR PRO; PR:Q6ZQ18; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q6ZQ18; protein.
DR Bgee; ENSMUSG00000020658; Expressed in central gray substance of midbrain and 178 other tissues.
DR ExpressionAtlas; Q6ZQ18; baseline and differential.
DR Genevisible; Q6ZQ18; MM.
DR GO; GO:0015629; C:actin cytoskeleton; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0046854; P:phosphatidylinositol phosphate biosynthetic process; ISS:UniProtKB.
DR GO; GO:0072659; P:protein localization to plasma membrane; ISS:UniProtKB.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cytoplasm; Lipoprotein; Membrane;
KW Palmitate; Phosphoprotein; Reference proteome.
FT CHAIN 1..817
FT /note="Protein EFR3 homolog B"
FT /id="PRO_0000312296"
FT MOD_RES 212
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 214
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 216
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 575..618
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14621295"
FT /id="VSP_029808"
SQ SEQUENCE 817 AA; 92406 MW; 84726F2CF744145B CRC64;
MYGVCGCCGA LRPRYKRLVD NIFPEDPEDG LVKTNMEKLT FYALSAPEKL DRIGAYLSER
LIRDVGRHRY GYVCIAMEAL DQLLMACHCQ SINLFVESFL KMVAKLLESE KPNLQILGTN
SFVKFANIEE DTPSYHRSYD FFVSRFSEMC HSSHDDLEIK TKIRMSGIKG LQGVVRKTVN
DELQANIWDP QHMDKIVPSL LFNLQHVEEA ESRSPSPLQA PEKEKENPAE LAERCLRELL
GRAAFGNIKN AIKPVLIHLD NHSLWEPKVF ATRCFKIIMY SIQPQHSHLV IQQLLSHLDA
NSRSAATVRA GIVEVLSEAA IIAATGSVGP TVLEMFNTLL RQLRLSIDYA LTGSYDGAVS
LGSKIIKEHE ECMFQEAVIK TIGSFASTLP TYQRSEVILF IMSKVPLPSV HHPVETGRTG
ENRNRLTQIM LLKSLLQVST GFQCNNMMSA LPSNFLDRLL STALMEDAEI RLFVLEILIS
FIDRHGNRHK FSTISTLGDI SVLKLKVDKC SRQDTVFMKK HSQQLYRHIY LSCKEETNIQ
KHYEALYGLL ALISIELANE EVVVDLIRLV LAVQDVAQVN EENLPTYNRC ALYALGAAYL
NLISQLTTVP AFCQHIHEVI ETRKKEAPYM LPEDVFVEKP RLSQNLDGVV IEFLFRQSKI
SEVLGGSGYN SDRLCLPYIP QLTDEDRLSK RKSIGETISL QVEVESRNSP EKEERVPAEE
ITYETLKKAI VDSVAVEEQE RERQRQVVEK FQKAPFEEIA AHCGARASLL QSKLNQIFEI
TIRPPPSPSG TISAAYGQPQ NHSIPVYEMK FPDLCVY
