EFR3_DROME
ID EFR3_DROME Reviewed; 834 AA.
AC Q8IGJ0; A1Z7I7; A1Z7I8; A1Z7I9; O96648; Q7K2E8;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 3.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Protein EFR3 homolog cmp44E;
DE AltName: Full=Conserved membrane protein at 44E;
DE AltName: Full=Protein stambha A;
GN Name=stmA; Synonyms=cmp44E; ORFNames=CG8739;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=9883579;
RX DOI=10.1002/(sici)1520-6408(1998)23:4<264::aid-dvg2>3.0.co;2-6;
RA Faulkner D.L., Dockendorff T.C., Jongens T.A.;
RT "Clonal analysis of cmp44E, which encodes a conserved putative
RT transmembrane protein, indicates a requirement for cell viability in
RT Drosophila.";
RL Dev. Genet. 23:264-274(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND C).
RC STRAIN=Berkeley; TISSUE=Embryo, and Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: An essential gene required for embryogenesis; required for
CC cell viability. {ECO:0000269|PubMed:9883579}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Comment=Named isoforms=3.;
CC Name=C;
CC IsoId=Q8IGJ0-2; Sequence=Displayed;
CC Name=A;
CC IsoId=Q8IGJ0-1; Sequence=VSP_022220, VSP_022221;
CC Name=B;
CC IsoId=Q8IGJ0-3; Sequence=VSP_022221;
CC -!- TISSUE SPECIFICITY: Expression during embryogenesis is ubiquitous with
CC notably higher levels in the CNS and brain.
CC {ECO:0000269|PubMed:9883579}.
CC -!- SIMILARITY: Belongs to the EFR3 family. {ECO:0000305}.
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DR EMBL; AF099028; AAD12254.1; -; mRNA.
DR EMBL; AE013599; AAF59046.2; -; Genomic_DNA.
DR EMBL; AE013599; AAF59047.2; -; Genomic_DNA.
DR EMBL; AE013599; AAX52723.1; -; Genomic_DNA.
DR EMBL; AY061062; AAL28610.1; -; mRNA.
DR EMBL; BT001762; AAN71517.1; -; mRNA.
DR RefSeq; NP_001014513.1; NM_001014513.2. [Q8IGJ0-2]
DR RefSeq; NP_724710.1; NM_165624.2. [Q8IGJ0-3]
DR RefSeq; NP_724711.1; NM_165625.2. [Q8IGJ0-1]
DR AlphaFoldDB; Q8IGJ0; -.
DR BioGRID; 61713; 5.
DR IntAct; Q8IGJ0; 5.
DR STRING; 7227.FBpp0100147; -.
DR SwissPalm; Q8IGJ0; -.
DR PaxDb; Q8IGJ0; -.
DR PRIDE; Q8IGJ0; -.
DR DNASU; 35865; -.
DR EnsemblMetazoa; FBtr0088715; FBpp0087794; FBgn0086784. [Q8IGJ0-3]
DR EnsemblMetazoa; FBtr0088716; FBpp0087795; FBgn0086784. [Q8IGJ0-1]
DR EnsemblMetazoa; FBtr0301639; FBpp0100147; FBgn0086784. [Q8IGJ0-2]
DR GeneID; 35865; -.
DR KEGG; dme:Dmel_CG8739; -.
DR CTD; 35865; -.
DR FlyBase; FBgn0086784; stmA.
DR VEuPathDB; VectorBase:FBgn0086784; -.
DR eggNOG; KOG1877; Eukaryota.
DR GeneTree; ENSGT00390000002143; -.
DR InParanoid; Q8IGJ0; -.
DR PhylomeDB; Q8IGJ0; -.
DR BioGRID-ORCS; 35865; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 35865; -.
DR PRO; PR:Q8IGJ0; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0086784; Expressed in brain and 29 other tissues.
DR ExpressionAtlas; Q8IGJ0; baseline and differential.
DR Genevisible; Q8IGJ0; DM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0098793; C:presynapse; IEA:GOC.
DR GO; GO:0045202; C:synapse; IDA:FlyBase.
DR GO; GO:0150008; P:bulk synaptic vesicle endocytosis; IMP:FlyBase.
DR GO; GO:0007268; P:chemical synaptic transmission; IMP:FlyBase.
DR GO; GO:0007602; P:phototransduction; IMP:FlyBase.
DR GO; GO:0072659; P:protein localization to plasma membrane; IBA:GO_Central.
DR GO; GO:0048488; P:synaptic vesicle endocytosis; IDA:FlyBase.
DR GO; GO:0016079; P:synaptic vesicle exocytosis; IMP:FlyBase.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..834
FT /note="Protein EFR3 homolog cmp44E"
FT /id="PRO_0000270768"
FT TRANSMEM 595..612
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 120..156
FT /note="HEAT"
FT VAR_SEQ 2..29
FT /note="ALIRCCFEPPELPEFFDSFVQKCTDPSC -> PG (in isoform A)"
FT /evidence="ECO:0000303|PubMed:12537569,
FT ECO:0000303|PubMed:9883579"
FT /id="VSP_022220"
FT VAR_SEQ 231..244
FT /note="Missing (in isoform A and isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569,
FT ECO:0000303|PubMed:9883579"
FT /id="VSP_022221"
FT CONFLICT 29
FT /note="C -> S (in Ref. 4; AAN71517)"
FT /evidence="ECO:0000305"
FT CONFLICT 83
FT /note="L -> P (in Ref. 4; AAN71517)"
FT /evidence="ECO:0000305"
FT CONFLICT 500
FT /note="S -> T (in Ref. 1; AAD12254)"
FT /evidence="ECO:0000305"
FT CONFLICT 671
FT /note="M -> I (in Ref. 4; AAN71517)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 834 AA; 93961 MW; B0F8059B50279C33 CRC64;
MALIRCCFEP PELPEFFDSF VQKCTDPSCC CGCCSALRPR YKRLVDNIFP VNPEDGLVKS
NMEKLTFYSL SSPDKLDRIG EYLYQKATKD INRKRYKLAE IAMEAMDLLL QACHAQTTLN
LFVESFLRMV QKLLEDSNPN LKIMATNSFV KFANINEDTP SYHRRYDFFI SKFSSMCHSD
AASMRDSLRL AGIKGLQGVI RKTVSDDLVE NIWEAEHMEK IVPSLLFNMQ FCVNVMFVKK
NLLASGDLTP VEDATNVTPP ALAEEVLREL VGRASFGHIR SVLKPLLTHL DRHELWVPNT
FAIHTFRIVM ISIQPQYSYT VVETLMQHLD NNFKSSPKTR TSLAVVLSKI IAIAAGESVG
PSALDIINNL LTHLRTSVST TSEITPEESQ YQEALINALG EFANHHPDYQ KIEIMLFIMN
TVPDLSKKSK GDQMLQNILL KSLLKVGTQY STVSFEKAFP ASFLQPLLKM ARAPHNPTRM
VVMQILQALL DRHQNEQVLS SVSVKPYPAL SQEPPSRSDI IFTHKYGANI MQALIDSMAL
SDRVDALTSS FNTAALLIVE MSCNETVQEF LLFILGIQQV ACTVDTLGNV HKCSLHAISI
GLLVLISRVS GINNLLEYAQ KIVDARREEA SHFLPPLLEP KKLAGKTFNL QLPHLAIDKL
ALGECLQNAG MDAQRLNTGA PYSLNQTDHP GHRHSWVESV SNQLTQRNSS ADLTVYNGDV
DSVSSSPGVC KKLLAPEFNF DAMKRALAEP TEAAKREQRE RQMQIVRTFR EGEFDDLMRR
TEPKHDLIQN RLNELFNSLA VERQITQSDT KSSQLQASNE KPIYETNFPE LFYY
