ID   3L237_OPHHA             Reviewed;          91 AA.
AC   Q53B59;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Long neurotoxin OH-37;
DE   Flags: Precursor;
OS   Ophiophagus hannah (King cobra) (Naja hannah).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Ophiophagus.
OX   NCBI_TaxID=8665;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-38, TOXIC DOSE, AND
RP   SUBCELLULAR LOCATION.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=15302536; DOI=10.1016/j.toxicon.2004.06.003;
RA   He Y.-Y., Lee W.-H., Zhang Y.;
RT   "Cloning and purification of alpha-neurotoxins from king cobra (Ophiophagus
RT   hannah).";
RL   Toxicon 44:295-303(2004).
CC   -!- FUNCTION: Binds with high affinity to muscular (alpha-1/CHRNA1) and
CC       neuronal (alpha-7/CHRNA7) nicotinic acetylcholine receptor (nAChR) and
CC       inhibits acetylcholine from binding to the receptor, thereby impairing
CC       neuromuscular and neuronal transmission.
CC       {ECO:0000250|UniProtKB:P60615}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- TOXIC DOSE: LD(50) is 110 ug/kg by intraperitoneal injection into mice.
CC       {ECO:0000269|PubMed:15302536}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Long-chain
CC       subfamily. Type II alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR   EMBL; AY596927; AAT97249.1; -; mRNA.
DR   AlphaFoldDB; Q53B59; -.
DR   SMR; Q53B59; -.
DR   TopDownProteomics; Q53B59; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:15302536"
FT   CHAIN           22..91
FT                   /note="Long neurotoxin OH-37"
FT                   /id="PRO_5000093320"
FT   DISULFID        24..41
FT                   /evidence="ECO:0000250"
FT   DISULFID        34..62
FT                   /evidence="ECO:0000250"
FT   DISULFID        47..51
FT                   /evidence="ECO:0000250"
FT   DISULFID        66..77
FT                   /evidence="ECO:0000250"
FT   DISULFID        78..83
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   91 AA;  9853 MW;  6CBC231DF4E97647 CRC64;
     MKTLLLTLVV MTIVCLDLGY SLICFISPHD SVTCAPGENV CFLKSWCDAW CGSRGKKLSF
     GCAATCPKVN PGIDIECCST DNCNPHPKLR P