ID   AFAC_ECOLX              Reviewed;         859 AA.
AC   P53517;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Outer membrane usher protein AfaC;
DE   Flags: Precursor;
GN   Name=afaC;
OS   Escherichia coli.
OG   Plasmid pIL1055.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A30 / UPEC;
RX   PubMed=8002584; DOI=10.1128/jb.176.24.7601-7613.1994;
RA   Garcia M.-I., Labigne A., le Bouguenec C.L.;
RT   "Nucleotide sequence of the afimbrial-adhesin-encoding afa-3 gene cluster
RT   and its translocation via flanking IS1 insertion sequences.";
RL   J. Bacteriol. 176:7601-7613(1994).
CC   -!- FUNCTION: Involved in the export and assembly of AFA-III afimbrial
CC       adhesin subunits across the outer membrane.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the fimbrial export usher family. {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-9 is the initiator.
CC       {ECO:0000305}.
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DR   EMBL; X76688; CAA54117.1; -; Genomic_DNA.
DR   PIR; F55545; F55545.
DR   AlphaFoldDB; P53517; -.
DR   SMR; P53517; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015473; F:fimbrial usher porin activity; IEA:InterPro.
DR   GO; GO:0009297; P:pilus assembly; IEA:InterPro.
DR   Gene3D; 2.60.40.2070; -; 1.
DR   Gene3D; 2.60.40.2610; -; 1.
DR   Gene3D; 3.10.20.410; -; 1.
DR   InterPro; IPR000015; Fimb_usher.
DR   InterPro; IPR018030; Fimbrial_membr_usher_CS.
DR   InterPro; IPR042186; FimD_plug_dom.
DR   InterPro; IPR025949; PapC-like_C.
DR   InterPro; IPR043142; PapC-like_C_sf.
DR   InterPro; IPR025885; PapC_N.
DR   InterPro; IPR037224; PapC_N_sf.
DR   PANTHER; PTHR30451; PTHR30451; 1.
DR   Pfam; PF13953; PapC_C; 1.
DR   Pfam; PF13954; PapC_N; 1.
DR   Pfam; PF00577; Usher; 1.
DR   SUPFAM; SSF141729; SSF141729; 1.
DR   PROSITE; PS01151; FIMBRIAL_USHER; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Membrane; Plasmid; Signal; Transmembrane;
KW   Transmembrane beta strand; Transport.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..859
FT                   /note="Outer membrane usher protein AfaC"
FT                   /id="PRO_0000009298"
SQ   SEQUENCE   859 AA;  93805 MW;  1FF7FE3715EEFB93 CRC64;
     MRDTSSGRMR TGVTGLALAV MVACVMFRAE SGIARTYSFD AAMLKGGGKG VDLTLFEEGG
     QLPGIYPVDI ILNGSRVDSQ EMAFHAERDA EGRPYLKTCL TREMLARYGV RIEEYPALFR
     ASGEGRGASV AEEACADLTA IPQATESYQF AAQQLVLGIP QVAPSAAEGD WPEALWDDGI
     PAFLLNWQAN AGRSEYRGYG KRVTDSYWVS LQPGINIGPW RVRNLTTWNR SSGQSGKWES
     SYIRAERGLN GIKSRLTLGE DYTPSDIFDS VPFRGAMMSS DESMVPYNLR EFAPVVRGIA
     RTQARIEVRQ NGYLIQSQTV APGAFALTDL PVTGSGSDLQ VTVLESDGTA QVFTVPFTTP
     AIALREGYLK YNVTAGQYRS SDDAVEHTSL GQVTAMYGLP WGLTVYGGLQ GADDYQSAAL
     GLGWSLGRLG AVSLDTTHSR GQQKGHDYET GDTWRIRYNK SFELTGTSFT AASYQYSSDG
     YHTLPDVLDT WRDDRYAYRH TENRSRRTTL SLSQSLGQWG YVGLNGSRDE YRDRPHRDYF
     GASYSTSWNN ISLSVNWSRN RNSGGYYGGW SRTEDSVSMW MSVPLGRWFG GADNDISTTA
     QMQRSTGQDT RYEAGLNGRA FDRRLYWDVR EQMVPGSESH ADTSRLNLTW YGTYGELTGM
     YSYSSTMRQL NAGMSGSMVA HSEGVTFGQR TGDTVALIAA PGVSGASVGG WPGVRTDFRG
     YTLAGYASPY QENVLTLDPT TFPEDAEVPQ TDSRVVPTKG AVVRAGFRTR VGGRALVSLA
     RQDGTPLPFG AVVTVEGERG QAAGSAGVVG DRGEVYLSGL KESGKLKAQW GENSLCHADY
     RLPEEKGPAG IFLTRTVCM