ID   AFAD_ECOLX              Reviewed;         147 AA.
AC   Q47038; Q47039; Q47040;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Protein AfaD;
DE   Flags: Precursor;
GN   Name=afaD;
OS   Escherichia coli.
OG   Plasmid pIL1055.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A30 / UPEC;
RX   PubMed=8002584; DOI=10.1128/jb.176.24.7601-7613.1994;
RA   Garcia M.-I., Labigne A., le Bouguenec C.L.;
RT   "Nucleotide sequence of the afimbrial-adhesin-encoding afa-3 gene cluster
RT   and its translocation via flanking IS1 insertion sequences.";
RL   J. Bacteriol. 176:7601-7613(1994).
CC   -!- SIMILARITY: To E.coli AggB. {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1, Met-8 or Met-9 is the
CC       initiator. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA54119.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAA54120.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X76688; CAA54118.1; -; Genomic_DNA.
DR   EMBL; X76688; CAA54119.1; ALT_INIT; Genomic_DNA.
DR   EMBL; X76688; CAA54120.1; ALT_INIT; Genomic_DNA.
DR   PIR; G55545; G55545.
DR   RefSeq; WP_001539577.1; NZ_WSWU01000146.1.
DR   PDB; 2AXW; X-ray; 1.05 A; A/B=27-147.
DR   PDB; 2FVN; NMR; -; A=27-147.
DR   PDB; 2IXQ; NMR; -; A=27-147.
DR   PDBsum; 2AXW; -.
DR   PDBsum; 2FVN; -.
DR   PDBsum; 2IXQ; -.
DR   AlphaFoldDB; Q47038; -.
DR   BMRB; Q47038; -.
DR   SMR; Q47038; -.
DR   EvolutionaryTrace; Q47038; -.
DR   Gene3D; 2.60.40.1570; -; 1.
DR   InterPro; IPR008966; Adhesion_dom_sf.
DR   InterPro; IPR008394; AfaD.
DR   InterPro; IPR037028; Dr_adhesin_sf.
DR   Pfam; PF05775; AfaD; 1.
DR   SUPFAM; SSF49401; SSF49401; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Plasmid; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..147
FT                   /note="Protein AfaD"
FT                   /id="PRO_0000020636"
FT   REGION          91..111
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   STRAND          28..33
FT                   /evidence="ECO:0007829|PDB:2AXW"
FT   STRAND          46..53
FT                   /evidence="ECO:0007829|PDB:2AXW"
FT   STRAND          59..69
FT                   /evidence="ECO:0007829|PDB:2AXW"
FT   STRAND          74..81
FT                   /evidence="ECO:0007829|PDB:2AXW"
FT   STRAND          82..85
FT                   /evidence="ECO:0007829|PDB:2FVN"
FT   STRAND          87..93
FT                   /evidence="ECO:0007829|PDB:2AXW"
FT   STRAND          103..105
FT                   /evidence="ECO:0007829|PDB:2FVN"
FT   STRAND          107..111
FT                   /evidence="ECO:0007829|PDB:2AXW"
FT   STRAND          114..127
FT                   /evidence="ECO:0007829|PDB:2AXW"
FT   STRAND          131..147
FT                   /evidence="ECO:0007829|PDB:2AXW"
SQ   SEQUENCE   147 AA;  15981 MW;  78227B132A7ED3F7 CRC64;
     MNGSIRKMMR VTCGMLLMVM SGVSQAAELH LESRGGSGTQ LRDGAKVATG RIICREAHTG
     FHVWMNERQV DGRAERYVVQ SKDGRHELRV RTGGDGWSPV KGEGGKGVSR PGQEEQVFFD
     VMADGNQDIA PGEYRFSVGG ACVVPQE