EFTS_THET8
ID EFTS_THET8 Reviewed; 196 AA.
AC P43895; Q5SJZ2;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 25-MAY-2022, entry version 141.
DE RecName: Full=Elongation factor Ts;
DE Short=EF-Ts;
GN Name=tsf; OrderedLocusNames=TTHA0860;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8617268; DOI=10.1111/j.1432-1033.1996.00222.x;
RA Blank J., Nock S., Kreutzer R., Sprinzl M.;
RT "Elongation factor Ts from Thermus thermophilus -- overproduction in
RT Escherichia coli, quaternary structure and interaction with elongation
RT factor Tu.";
RL Eur. J. Biochem. 236:222-227(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF COMPLEX WITH EF-TU.
RX PubMed=9253415; DOI=10.1038/nsb0897-650;
RA Wang Y., Jiang Y., Meyering-Voss M., Sprinzl M., Sigler P.B.;
RT "Crystal structure of the EF-Tu.EF-Ts complex from Thermus thermophilus.";
RL Nat. Struct. Biol. 4:650-656(1997).
CC -!- FUNCTION: Associates with the EF-Tu.GDP complex and induces the
CC exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-
CC Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.
CC -!- SUBUNIT: Heterotetramer composed of two EF-Ts.EF-Tu dimer complexes.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the EF-Ts family. {ECO:0000305}.
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DR EMBL; X83598; CAA58578.1; -; Genomic_DNA.
DR EMBL; AP008226; BAD70683.1; -; Genomic_DNA.
DR RefSeq; WP_011228249.1; NC_006461.1.
DR RefSeq; YP_144126.1; NC_006461.1.
DR PDB; 1AIP; X-ray; 3.00 A; C/D/G/H=1-196.
DR PDB; 1TFE; X-ray; 1.70 A; A=55-196.
DR PDBsum; 1AIP; -.
DR PDBsum; 1TFE; -.
DR AlphaFoldDB; P43895; -.
DR SMR; P43895; -.
DR DIP; DIP-6078N; -.
DR IntAct; P43895; 1.
DR STRING; 300852.55772242; -.
DR EnsemblBacteria; BAD70683; BAD70683; BAD70683.
DR GeneID; 3170120; -.
DR KEGG; ttj:TTHA0860; -.
DR PATRIC; fig|300852.9.peg.854; -.
DR eggNOG; COG0264; Bacteria.
DR HOGENOM; CLU_047155_1_1_0; -.
DR OMA; DAGMMDC; -.
DR PhylomeDB; P43895; -.
DR EvolutionaryTrace; P43895; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.479.20; -; 1.
DR HAMAP; MF_00050; EF_Ts; 1.
DR InterPro; IPR036402; EF-Ts_dimer_sf.
DR InterPro; IPR001816; Transl_elong_EFTs/EF1B.
DR InterPro; IPR014039; Transl_elong_EFTs/EF1B_dimer.
DR InterPro; IPR018101; Transl_elong_Ts_CS.
DR InterPro; IPR009060; UBA-like_sf.
DR PANTHER; PTHR11741; PTHR11741; 1.
DR Pfam; PF00889; EF_TS; 1.
DR SUPFAM; SSF46934; SSF46934; 1.
DR SUPFAM; SSF54713; SSF54713; 1.
DR TIGRFAMs; TIGR00116; tsf; 1.
DR PROSITE; PS01126; EF_TS_1; 1.
DR PROSITE; PS01127; EF_TS_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Elongation factor; Protein biosynthesis;
KW Reference proteome.
FT CHAIN 1..196
FT /note="Elongation factor Ts"
FT /id="PRO_0000161222"
FT REGION 80..83
FT /note="Involved in Mg(2+) ion dislocation from EF-Tu"
FT /evidence="ECO:0000250"
FT HELIX 3..14
FT /evidence="ECO:0007829|PDB:1AIP"
FT HELIX 18..27
FT /evidence="ECO:0007829|PDB:1AIP"
FT TURN 28..30
FT /evidence="ECO:0007829|PDB:1AIP"
FT HELIX 32..50
FT /evidence="ECO:0007829|PDB:1AIP"
FT STRAND 57..64
FT /evidence="ECO:0007829|PDB:1TFE"
FT STRAND 68..79
FT /evidence="ECO:0007829|PDB:1TFE"
FT HELIX 81..85
FT /evidence="ECO:0007829|PDB:1TFE"
FT HELIX 87..103
FT /evidence="ECO:0007829|PDB:1TFE"
FT STRAND 106..109
FT /evidence="ECO:0007829|PDB:1TFE"
FT HELIX 110..112
FT /evidence="ECO:0007829|PDB:1TFE"
FT HELIX 115..130
FT /evidence="ECO:0007829|PDB:1TFE"
FT TURN 131..133
FT /evidence="ECO:0007829|PDB:1TFE"
FT HELIX 136..154
FT /evidence="ECO:0007829|PDB:1TFE"
FT HELIX 156..158
FT /evidence="ECO:0007829|PDB:1TFE"
FT STRAND 159..161
FT /evidence="ECO:0007829|PDB:1TFE"
FT STRAND 164..168
FT /evidence="ECO:0007829|PDB:1TFE"
FT HELIX 169..180
FT /evidence="ECO:0007829|PDB:1TFE"
FT STRAND 185..193
FT /evidence="ECO:0007829|PDB:1TFE"
SQ SEQUENCE 196 AA; 22413 MW; 36A771F686BBB0DD CRC64;
MSQMELIKKL REATGAGMMD VKRALEDAGW DEEKAVQLLR ERGAMKAAKK ADREAREGII
GHYIHHNQRV GVLVELNCET DFVARNELFQ NLAKDLAMHI AMMNPRYVSA EEIPAEELEK
ERQIYIQAAL NEGKPQQIAE KIAEGRLKKY LEEVVLLEQP FVKDDKVKVK ELIQQAIAKI
GENIVVRRFC RFELGA
