EFTU1_PSEPK
ID EFTU1_PSEPK Reviewed; 397 AA.
AC Q88QP8;
DT 24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Elongation factor Tu-A {ECO:0000255|HAMAP-Rule:MF_00118};
DE Short=EF-Tu-A {ECO:0000255|HAMAP-Rule:MF_00118};
GN Name=tufA {ECO:0000255|HAMAP-Rule:MF_00118}; Synonyms=tuf-1;
GN OrderedLocusNames=PP_0440;
OS Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS / KT2440).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=160488;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT "Complete genome sequence and comparative analysis of the metabolically
RT versatile Pseudomonas putida KT2440.";
RL Environ. Microbiol. 4:799-808(2002).
CC -!- FUNCTION: This protein promotes the GTP-dependent binding of aminoacyl-
CC tRNA to the A-site of ribosomes during protein biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00118}.
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00118}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00118}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00118}.
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DR EMBL; AE015451; AAN66070.1; -; Genomic_DNA.
DR RefSeq; NP_742606.1; NC_002947.4.
DR RefSeq; WP_003255489.1; NC_002947.4.
DR PDB; 4IW3; X-ray; 2.70 A; B/K=1-397.
DR PDB; 4J0Q; X-ray; 2.29 A; A/B/C/D/E=1-397.
DR PDBsum; 4IW3; -.
DR PDBsum; 4J0Q; -.
DR AlphaFoldDB; Q88QP8; -.
DR SMR; Q88QP8; -.
DR STRING; 160488.PP_0440; -.
DR PRIDE; Q88QP8; -.
DR EnsemblBacteria; AAN66070; AAN66070; PP_0440.
DR KEGG; ppu:PP_0440; -.
DR PATRIC; fig|160488.4.peg.471; -.
DR eggNOG; COG0050; Bacteria.
DR HOGENOM; CLU_007265_0_0_6; -.
DR OMA; EGDKEWG; -.
DR PhylomeDB; Q88QP8; -.
DR BioCyc; PPUT160488:G1G01-482-MON; -.
DR Proteomes; UP000000556; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd01884; EF_Tu; 1.
DR CDD; cd03697; EFTU_II; 1.
DR DisProt; DP01571; -.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00118_B; EF_Tu_B; 1.
DR InterPro; IPR041709; EF-Tu_GTP-bd.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR033720; EFTU_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004541; Transl_elong_EFTu/EF1A_bac/org.
DR InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF03143; GTP_EFTU_D3; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF50465; SSF50465; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00485; EF-Tu; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Elongation factor; GTP-binding;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..397
FT /note="Elongation factor Tu-A"
FT /id="PRO_0000091366"
FT DOMAIN 10..207
FT /note="tr-type G"
FT REGION 19..26
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 60..64
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 81..84
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 136..139
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 174..176
FT /note="G5"
FT /evidence="ECO:0000250"
FT BINDING 19..26
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00118"
FT BINDING 81..85
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00118"
FT BINDING 136..139
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00118"
FT STRAND 12..20
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 21..24
FT /evidence="ECO:0007829|PDB:4IW3"
FT HELIX 25..39
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 66..71
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 76..81
FT /evidence="ECO:0007829|PDB:4J0Q"
FT HELIX 85..94
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 102..107
FT /evidence="ECO:0007829|PDB:4J0Q"
FT TURN 108..110
FT /evidence="ECO:0007829|PDB:4J0Q"
FT HELIX 114..126
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 131..136
FT /evidence="ECO:0007829|PDB:4J0Q"
FT HELIX 138..140
FT /evidence="ECO:0007829|PDB:4J0Q"
FT HELIX 144..160
FT /evidence="ECO:0007829|PDB:4J0Q"
FT TURN 165..167
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 170..172
FT /evidence="ECO:0007829|PDB:4J0Q"
FT HELIX 175..179
FT /evidence="ECO:0007829|PDB:4J0Q"
FT HELIX 184..186
FT /evidence="ECO:0007829|PDB:4J0Q"
FT HELIX 189..202
FT /evidence="ECO:0007829|PDB:4J0Q"
FT HELIX 209..211
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 215..217
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 221..224
FT /evidence="ECO:0007829|PDB:4J0Q"
FT TURN 225..227
FT /evidence="ECO:0007829|PDB:4IW3"
FT STRAND 229..234
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 237..241
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 245..252
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 255..264
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 267..273
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 277..284
FT /evidence="ECO:0007829|PDB:4J0Q"
FT HELIX 287..289
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 295..298
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 304..314
FT /evidence="ECO:0007829|PDB:4J0Q"
FT TURN 317..320
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 326..329
FT /evidence="ECO:0007829|PDB:4IW3"
FT STRAND 333..336
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 339..346
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 352..354
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 359..371
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 377..382
FT /evidence="ECO:0007829|PDB:4J0Q"
FT STRAND 385..397
FT /evidence="ECO:0007829|PDB:4J0Q"
SQ SEQUENCE 397 AA; 43496 MW; 571E0854AA7EF96F CRC64;
MAKEKFDRSL PHVNVGTIGH VDHGKTTLTA ALTRVCSEVF GSAIVEFDKI DSAPEEKARG
ITINTAHVEY NSTIRHYAHV DCPGHADYVK NMITGAAQMD GAILVCSAAD GPMPQTREHI
LLSRQVGVPY IVVFLNKADL VDDAELLELV EMEVRDLLST YDFPGDDTPI IIGSARMALE
GKDDNEMGTT AVKKLVETLD SYIPEPVRAI DQPFLMPIED VFSISGRGTV VTGRIERGIV
RVQDPLEIVG LRDTTTTTCT GVEMFRKLLD EGRAGENCGV LLRGTKRDDV ERGQVLVKPG
SVKPHTKFTA EVYVLSKEEG GRHTPFFKGY RPQFYFRTTD VTGNCELPEG VEMVMPGDNI
QMTVTLIKTI AMEDGLRFAI REGGRTVGAG VVAKIIE
