AFF1_MOUSE
ID AFF1_MOUSE Reviewed; 1216 AA.
AC O88573;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=AF4/FMR2 family member 1;
DE AltName: Full=Proto-oncogene AF4;
DE Short=Protein AF-4;
GN Name=Aff1; Synonyms=Mllt2, Mllt2h;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Thymus;
RX PubMed=9880680; DOI=10.1007/s003359900927;
RA Isnard P., Depetris D., Mattei M.-G., Ferrier P., Djabali M.;
RT "cDNA cloning, expression and chromosomal localization of the murine AF-4
RT gene involved in human leukemia.";
RL Mamm. Genome 9:1065-1068(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183; SER-191; SER-197;
RP SER-755 AND SER-760, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBUNIT: Component of the super elongation complex (SEC), at least
CC composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb
CC complex and ELL (ELL, ELL2 or ELL3). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the AF4 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF074266; AAD08668.1; -; mRNA.
DR EMBL; AL713989; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL731554; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; T42625; T42625.
DR AlphaFoldDB; O88573; -.
DR SMR; O88573; -.
DR IntAct; O88573; 2.
DR MINT; O88573; -.
DR STRING; 10090.ENSMUSP00000059744; -.
DR iPTMnet; O88573; -.
DR PhosphoSitePlus; O88573; -.
DR jPOST; O88573; -.
DR MaxQB; O88573; -.
DR PaxDb; O88573; -.
DR PeptideAtlas; O88573; -.
DR PRIDE; O88573; -.
DR ProteomicsDB; 285768; -.
DR MGI; MGI:1100819; Aff1.
DR eggNOG; ENOG502QR32; Eukaryota.
DR InParanoid; O88573; -.
DR ChiTaRS; Aff1; mouse.
DR PRO; PR:O88573; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; O88573; protein.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0032783; C:super elongation complex; IBA:GO_Central.
DR GO; GO:0008023; C:transcription elongation factor complex; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006351; P:transcription, DNA-templated; TAS:MGI.
DR InterPro; IPR007797; AF4/FMR2.
DR InterPro; IPR043640; AF4/FMR2_CHD.
DR InterPro; IPR043639; AF4_int.
DR PANTHER; PTHR10528; PTHR10528; 1.
DR Pfam; PF18876; AF-4_C; 1.
DR Pfam; PF18875; AF4_int; 1.
PE 1: Evidence at protein level;
KW Acetylation; Nucleus; Phosphoprotein; Proto-oncogene; Reference proteome.
FT CHAIN 1..1216
FT /note="AF4/FMR2 family member 1"
FT /id="PRO_0000215911"
FT REGION 1..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 68..104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 116..139
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 152..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 244..275
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 352..728
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 777..969
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1094..1125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..37
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 68..93
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 160..178
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 200..217
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 254..269
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 380..411
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 428..442
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 444..470
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 471..486
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 497..540
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 647..665
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 702..728
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 786..817
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 827..886
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 900..915
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 916..930
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 183
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 191
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 197
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 682
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P51825"
FT MOD_RES 755
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 760
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 378
FT /note="P -> L (in Ref. 1; AAD08668)"
FT /evidence="ECO:0000305"
FT CONFLICT 619
FT /note="G -> R (in Ref. 1; AAD08668)"
FT /evidence="ECO:0000305"
FT CONFLICT 820
FT /note="K -> R (in Ref. 1; AAD08668)"
FT /evidence="ECO:0000305"
FT CONFLICT 838
FT /note="R -> KT (in Ref. 1; AAD08668)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1216 AA; 131558 MW; 4CCA54DBB4992E8B CRC64;
MAAHSSLYNE DRNLLRIREK ERRNQEAHQE KEAFPEKAPL FPEPYKTAKG DELSSRIQTM
LGDYEEMKEF LSSKSHPHRL DGSEDRPGKP RYPLGHDRGN GAASSSLRTH VYHQPIHTSA
PGSRPVGNIS HSPKMAQPRM EPSLHTKIYD GPRLTQDHLS QGHCSRKCDR RAEGDSAPER
KLSPLISSLP SPVPPLSPVH SRLQGTSKAH SSGVSSKSCC VAKSSKDLVA KAQDKETPHD
GLVAVTSLGS APPQPPCQTF PPPPLPSKSA AMQQKPTAYV RPMDGQDQAP SESPELKLPL
EDYGQQSFEK PDLKVPAKAK LTRLRMPSQS VEQPYSNEVH CVEEILKEMT HSWPPPLTAI
HTPSTAEPSR FPFPTKDPLH VSPATQSQKQ YDTPSKTHPN PQQGTSMLED DLQLSDSEDS
DTEQATEKPP SPPAPPSAPQ TLPEPVASAH SSSGESESSE SDSSSDSESE SSSSDSEEEE
ENEPLETRAP EPEPPTTNKW QLDNWLTKVN QPSVPLDGRG STESPQWRQE SKGVAEGSSD
QQHPDSKDPL PKSSSKTLRG PSEGPSLGRG AVRNPPLNRN PHLGKPWAAN NPGNPPRLRP
GRAQASSQAE SEVGPLPYGS KEQTSKDRPK VKTKGRPRAV GSREPKPEVP APTPQAAVPR
PKPPVPTPSE KRKHKSSTAP SKAPSAPQPP KDSAGDRNPE HSALVSLTQS QGPSHSSRGS
SGSVRTSGCR QAVIAQGDGC KDKLLLPLRD TKLLSPLRDS PPPTSLVVKI TLDLLTRIPQ
PLGKGSRPRK AEDKQLSAGK KQDSETKSCD SSSRVTKKRK VTQKKSTVTR DTNWISRRAS
SSSSHTESSR TKAPRSSSEN SRKEMLPPAS ASSVSSSSSS QKPSRPAQKR PRPDEDTCSQ
EPPRSASSTK SSSTDPPAPK HRKVQARGSE HKGSSGDAAN AANPFPVPSL PNGNAKPGKP
QVKSDRQQAD FHMKEAKKLK CKAETMVDKA GKAFKYLEAV LSFIECGMAS ESESSAKSAY
AVYSETIDLI RYVMSLKCFS DNTMPAQEKI FAVLCLRCQS LLNMAMFRCK KDTVMKYSRT
LSEHFKSTSK VAQAPSPCTA RSTGVPSPLS PMPSPASSVG SQSSAGSSMG SVGVTATVST
PVSIQNMTSS YVTITSHVLT AFSLWEQAEA LTRKNKEFFA QLSTKVRVLA LNSSLVDLVH
YTRQGLQRLK QSPKGP
