AFF3_MOUSE
ID AFF3_MOUSE Reviewed; 1254 AA.
AC P51827; Q80WS6;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=AF4/FMR2 family member 3;
DE AltName: Full=Lymphoid nuclear protein related to AF4;
DE Short=Protein LAF-4;
GN Name=Aff3; Synonyms=Laf4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-229.
RX PubMed=8555498;
RA Ma C., Staudt L.M.;
RT "LAF-4 encodes a lymphoid nuclear protein with transactivation potential
RT that is homologous to AF-4, the gene fused to MLL in t(4;11) leukemias.";
RL Blood 87:734-745(1996).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-782, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Putative transcription activator that may function in
CC lymphoid development and oncogenesis.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P51827-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P51827-2; Sequence=VSP_022225, VSP_022226;
CC -!- TISSUE SPECIFICITY: Highest levels found in lymphoid tissues, lower
CC levels in brain and lung.
CC -!- MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the AF4 family. {ECO:0000305}.
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DR EMBL; AC117789; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC122026; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC132381; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC138386; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC052061; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; U34361; AAA98764.1; -; mRNA.
DR AlphaFoldDB; P51827; -.
DR SMR; P51827; -.
DR IntAct; P51827; 2.
DR STRING; 10090.ENSMUSP00000092637; -.
DR iPTMnet; P51827; -.
DR PhosphoSitePlus; P51827; -.
DR jPOST; P51827; -.
DR PaxDb; P51827; -.
DR PRIDE; P51827; -.
DR ProteomicsDB; 285735; -. [P51827-1]
DR ProteomicsDB; 285736; -. [P51827-2]
DR Antibodypedia; 32831; 61 antibodies from 17 providers.
DR Ensembl; ENSMUST00000238906; ENSMUSP00000159147; ENSMUSG00000037138. [P51827-1]
DR UCSC; uc007asu.1; mouse. [P51827-1]
DR MGI; MGI:106927; Aff3.
DR VEuPathDB; HostDB:ENSMUSG00000037138; -.
DR eggNOG; ENOG502QU9C; Eukaryota.
DR GeneTree; ENSGT00950000182974; -.
DR InParanoid; P51827; -.
DR OMA; CGNSRTG; -.
DR PhylomeDB; P51827; -.
DR ChiTaRS; Aff3; mouse.
DR PRO; PR:P51827; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P51827; protein.
DR Bgee; ENSMUSG00000037138; Expressed in vas deferens and 249 other tissues.
DR ExpressionAtlas; P51827; baseline and differential.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:BHF-UCL.
DR GO; GO:0032783; C:super elongation complex; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:MGI.
DR GO; GO:0003690; F:double-stranded DNA binding; IDA:MGI.
DR GO; GO:0035116; P:embryonic hindlimb morphogenesis; IEP:BHF-UCL.
DR GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0034612; P:response to tumor necrosis factor; ISO:MGI.
DR GO; GO:0006351; P:transcription, DNA-templated; TAS:MGI.
DR InterPro; IPR007797; AF4/FMR2.
DR InterPro; IPR043640; AF4/FMR2_CHD.
DR InterPro; IPR043639; AF4_int.
DR PANTHER; PTHR10528; PTHR10528; 1.
DR Pfam; PF18876; AF-4_C; 1.
DR Pfam; PF18875; AF4_int; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..1254
FT /note="AF4/FMR2 family member 3"
FT /id="PRO_0000215917"
FT REGION 45..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 139..190
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 261..324
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 350..534
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 552..752
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 813..883
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 919..991
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1128..1171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 46..64
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 65..80
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 142..162
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 413..477
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 659..690
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 691..705
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 715..752
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 838..867
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 869..883
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 924..984
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1128..1146
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1153..1171
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 782
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 908
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P51826"
FT VAR_SEQ 407
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_022225"
FT VAR_SEQ 564..1254
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_022226"
FT CONFLICT 18..51
FT /note="WGEDILSQRNDSLVLEVQASASRCRSVYEPDRNA -> CVSITGQEV (in
FT Ref. 3; AAA98764)"
FT /evidence="ECO:0000305"
FT CONFLICT 79..80
FT /note="SE -> RQ (in Ref. 3; AAA98764)"
FT /evidence="ECO:0000305"
FT CONFLICT 84
FT /note="T -> S (in Ref. 3; AAA98764)"
FT /evidence="ECO:0000305"
FT CONFLICT 145
FT /note="P -> S (in Ref. 3; AAA98764)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1254 AA; 136131 MW; 240E0CCB568B9478 CRC64;
MDSFDLALLQ EWDLESLWGE DILSQRNDSL VLEVQASASR CRSVYEPDRN ALRRKERERR
SQETQQDSGS FNSGYSLFSE PYKTNKGDEL SNRIQNTLGN YDEMKDFLTD RSNQSHLVGV
PKPGVPQTPV NKIDEHFGAE SRAQPQPSTV CSTASSTPAA VPVQQGKRGA MGWQKAGHPP
SDGQQRAAQQ GSLRTLLGDG VGRQQTRAKQ VCNMETGLQT QERPPAMAAK HGGSGHCVQN
FPPSLASKPS LVQQKPTAYV RPMDGQDQAP DESPKLKSST ETAVHCTAYR GVPANKPESA
RAKAKLAKFS IPKQAEESRS GENNSCVEEI IREMTWLPPL SAIQAPAKVE PSKFPFPNKD
SQLVSSGHSN PKKADAEPGS PDNGASNTST LEDDLKLSSD DEEGEQQAAQ RTALRALADS
SVVQQTNCRG SAPSSKGGGS SSSSGGSSSS SDSESTSGSD SETESSSSSS ESEGSKPPHC
SSPEAEPASS NKWQLDKWLN KVNPHKPPIL IQNESHGPER NQYYTPPVKD EGQDCGKLPE
ICQASLRDKE LKTTCKEEQR PRTANKAPGS KSVKQKSPPA AVAVTAAALP PAVPSAPTES
APAPTRRSAG KKPTRRTERT SAGDGANCHR PEEPVAPDTL GASVVGPLEP PKTRPCGNNR
TGHRKELRSS VTCEKRRTRG LSRIVPKSKE FIETESSSSS SSSDSDLESE QEEYVLSKAP
TTTGSEQRLK EAASSNNNSN SNSSTSRASV GSINARTTSD IAKELEEQFY TLVPFGRNEL
LSPLKDSDEV RSLWVKIDLT LLSRIPEHLS QEPGVLSAPS AKDTDSAPAS HALDAPAEKT
LPKSKRKRKC DNEDDYREIK KVQGRKESAS RLAASTNNTL SGNHCNVNVN SLAIPINKNE
KMLRSPTSPL SDTCKHKYAS EDLTSSSRPH GNGLLTSASS NKEPKAESQL QTIAGDLTKA
SHNSSENGTL HSKSRPQTEP WSPGSNGHRD CKRQKLIFDD MPRSADYFMR EAKRMKHKAD
AMVEKFGKAL NYAEAALSFI ECGNAMEQGP MESKSPYTMY SETVELIRYA MRLKTHSGPN
ATPEDKQLAA LCYRCLALLY WRMFRLKRDH AVKYSKALID YFKNSSKAAQ APSPWGSSGK
STGSPSPMSP NPSPASSVGS QGSLSSSSGL SPSTIVSIPQ RIHQMAANHV SITNSILHSY
DYWEMADNLA KENREFFNDL DLLMGPVTLH SSMEHLVQYS QQGLHWLRSS VHLS
