AFG1_YEAST
ID AFG1_YEAST Reviewed; 509 AA.
AC P32317; D3DLJ8; E9P923;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Protein AFG1;
GN Name=AFG1; Synonyms=AFG; OrderedLocusNames=YEL052W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-481.
RX PubMed=1441755; DOI=10.1002/yea.320080912;
RA Lee Y.-J., Wickner R.B.;
RT "AFG1, a new member of the SEC18-NSF, PAS1, CDC48-VCP, TBP family of
RT ATPases.";
RL Yeast 8:787-790(1992).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- MISCELLANEOUS: Present with 3570 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the AFG1 ATPase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA34414.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAB23997.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U18779; AAB64990.1; -; Genomic_DNA.
DR EMBL; M94535; AAA34414.1; ALT_FRAME; Genomic_RNA.
DR EMBL; AY693149; AAT93168.1; -; Genomic_DNA.
DR EMBL; S47813; AAB23997.1; ALT_FRAME; Genomic_DNA.
DR EMBL; BK006939; DAA07602.1; -; Genomic_DNA.
DR PIR; S30825; S30825.
DR RefSeq; NP_010862.1; NM_001178867.1.
DR AlphaFoldDB; P32317; -.
DR BioGRID; 36677; 52.
DR IntAct; P32317; 1.
DR STRING; 4932.YEL052W; -.
DR iPTMnet; P32317; -.
DR MaxQB; P32317; -.
DR PaxDb; P32317; -.
DR PRIDE; P32317; -.
DR EnsemblFungi; YEL052W_mRNA; YEL052W; YEL052W.
DR GeneID; 856658; -.
DR KEGG; sce:YEL052W; -.
DR SGD; S000000778; AFG1.
DR VEuPathDB; FungiDB:YEL052W; -.
DR eggNOG; KOG2383; Eukaryota.
DR GeneTree; ENSGT00390000013227; -.
DR HOGENOM; CLU_008681_1_1_1; -.
DR InParanoid; P32317; -.
DR OMA; QKREYWE; -.
DR BioCyc; YEAST:G3O-30170-MON; -.
DR PRO; PR:P32317; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P32317; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0034599; P:cellular response to oxidative stress; IMP:SGD.
DR GO; GO:0006515; P:protein quality control for misfolded or incompletely synthesized proteins; IMP:SGD.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR005654; ATPase_AFG1-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR12169; PTHR12169; 1.
DR Pfam; PF03969; AFG1_ATPase; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Nucleotide-binding; Reference proteome.
FT CHAIN 1..509
FT /note="Protein AFG1"
FT /id="PRO_0000064477"
FT BINDING 126..133
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 210
FT /note="T -> P (in Ref. 4; AAA34414/AAB23997)"
FT /evidence="ECO:0000305"
FT CONFLICT 373
FT /note="A -> R (in Ref. 4; AAA34414/AAB23997)"
FT /evidence="ECO:0000305"
FT CONFLICT 399
FT /note="V -> A (in Ref. 3; AAT93168)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 509 AA; 58265 MW; 630315D29D5A9924 CRC64;
MIALKPNAVR TFRQVQHCSF RICRYQSTKS NKCLTPLQEY DRLVKLGKLR DDTYQRGIIS
SLGDLYDSLV KYVPPVVKTP NAVDQVGGWL NGLKSVFSRG KPKNIGAYVD VSKIGNSIPR
GVYLYGDVGC GKTMLMDLFY TTIPNHLTKK RIHFHQFMQY VHKRSHEIVR EQNLKELGDA
KGKEIDTVPF LAAEIANNSH VLCFDEFQVT DVADAMILRR LMTALLSDDY GVVLFATSNR
HPDELYINGV QRQSFIPCIE LIKHRTKVIF LNSPTDYRKI PRPVSSVYYF PSDTSIKYAS
KECKTRRETH IKEWYNYFAQ ASHTDDSTDS HTVHKTFYDY PLTIWGREFK VPKCTPPRVA
QFTFKQLCGE PLAAGDYLTL AKNFEAFIVT DIPYLSIYVR DEVRRFITFL DAVYDSGGKL
ATTGAADFSS LFVEPEQILN DFELRPTTKE PDSVDTGMVD EMVEKHGFSK EIAKKSQMFA
LDEERFAFAR ALSRLSQMSS TDWVTKPTY
