EFTU_CHLT2
ID EFTU_CHLT2 Reviewed; 394 AA.
AC B0B7N8; O84324; P26622;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Elongation factor Tu {ECO:0000255|HAMAP-Rule:MF_00118};
DE Short=EF-Tu {ECO:0000255|HAMAP-Rule:MF_00118};
GN Name=tuf {ECO:0000255|HAMAP-Rule:MF_00118}; OrderedLocusNames=CTL0574;
OS Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B).
OC Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC Chlamydia/Chlamydophila group; Chlamydia.
OX NCBI_TaxID=471472;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1398121; DOI=10.1016/0378-1119(92)90006-b;
RA Cousineau B., Cerpa C., Lefebvre J., Cedergren R.;
RT "The sequence of the gene encoding elongation factor Tu from Chlamydia
RT trachomatis compared with those of other organisms.";
RL Gene 120:33-41(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=434/Bu / ATCC VR-902B;
RX PubMed=18032721; DOI=10.1101/gr.7020108;
RA Thomson N.R., Holden M.T.G., Carder C., Lennard N., Lockey S.J., Marsh P.,
RA Skipp P., O'Connor C.D., Goodhead I., Norbertzcak H., Harris B., Ormond D.,
RA Rance R., Quail M.A., Parkhill J., Stephens R.S., Clarke I.N.;
RT "Chlamydia trachomatis: genome sequence analysis of lymphogranuloma
RT venereum isolates.";
RL Genome Res. 18:161-171(2008).
RN [3]
RP PROTEIN SEQUENCE OF 2-11.
RA Bini L., Santucci A., Magi B., Marzocchi B., Sanchez-Campillo M.,
RA Comanducci M., Christianen G., Birkelund S., Vtretou E., Ratti G.,
RA Pallini V.;
RL Submitted (SEP-1994) to UniProtKB.
CC -!- FUNCTION: This protein promotes the GTP-dependent binding of aminoacyl-
CC tRNA to the A-site of ribosomes during protein biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00118}.
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00118}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00118}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00118}.
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DR EMBL; M74221; AAA62238.1; -; Genomic_DNA.
DR EMBL; AM884176; CAP04014.1; -; Genomic_DNA.
DR PIR; JC1420; JC1420.
DR RefSeq; WP_009873724.1; NC_010287.1.
DR RefSeq; YP_001654650.1; NC_010287.1.
DR AlphaFoldDB; B0B7N8; -.
DR SMR; B0B7N8; -.
DR PRIDE; B0B7N8; -.
DR EnsemblBacteria; CAP04014; CAP04014; CTL0574.
DR KEGG; ctb:CTL0574; -.
DR PATRIC; fig|471472.4.peg.615; -.
DR HOGENOM; CLU_007265_0_0_0; -.
DR OMA; EGDKEWG; -.
DR Proteomes; UP000000795; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd01884; EF_Tu; 1.
DR CDD; cd03697; EFTU_II; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00118_B; EF_Tu_B; 1.
DR InterPro; IPR041709; EF-Tu_GTP-bd.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR033720; EFTU_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004541; Transl_elong_EFTu/EF1A_bac/org.
DR InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF03143; GTP_EFTU_D3; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF50465; SSF50465; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00485; EF-Tu; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; Elongation factor; GTP-binding;
KW Nucleotide-binding; Protein biosynthesis.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|Ref.3"
FT CHAIN 2..394
FT /note="Elongation factor Tu"
FT /id="PRO_1000095056"
FT DOMAIN 10..204
FT /note="tr-type G"
FT REGION 19..26
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 60..64
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 81..84
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 136..139
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 174..176
FT /note="G5"
FT /evidence="ECO:0000250"
FT BINDING 19..26
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00118"
FT BINDING 81..85
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00118"
FT BINDING 136..139
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00118"
FT CONFLICT 11
FT /note="P -> D (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 17
FT /note="T -> A (in Ref. 1; AAA62238)"
FT /evidence="ECO:0000305"
FT CONFLICT 25
FT /note="K -> R (in Ref. 1; AAA62238)"
FT /evidence="ECO:0000305"
FT CONFLICT 62
FT /note="T -> P (in Ref. 1; AAA62238)"
FT /evidence="ECO:0000305"
FT CONFLICT 84
FT /note="G -> C (in Ref. 1; AAA62238)"
FT /evidence="ECO:0000305"
FT CONFLICT 255..259
FT /note="IVTGV -> LLLGL (in Ref. 1; AAA62238)"
FT /evidence="ECO:0000305"
FT CONFLICT 265..269
FT /note="ELPEG -> NSQKV (in Ref. 1; AAA62238)"
FT /evidence="ECO:0000305"
FT CONFLICT 334
FT /note="R -> L (in Ref. 1; AAA62238)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 394 AA; 43309 MW; 3F013074478170FD CRC64;
MSKETFQRNK PHINIGTIGH VDHGKTTLTA AITRTLSGDG LADFRDYSSI DNTPEEKARG
ITINASHVEY ETANRHYAHV DCPGHADYVK NMITGAAQMD GAILVVSATD GAMPQTKEHI
LLARQVGVPY IVVFLNKIDM ISEEDAELVD LVEMELAELL EEKGYKGCPI IRGSALKALE
GDAAYIEKVR ELMQAVDDNI PTPEREIDKP FLMPIEDVFS ISGRGTVVTG RIERGIVKVS
DKVQLVGLRD TKETIVTGVE MFRKELPEGR AGENVGLLLR GIGKNDVERG MVVCLPNSVK
PHTRFKCAVY VLQKEEGGRH KPFFTGYRPQ FFFRTTDVTG VVTLPEGVEM VMPGDNVEFE
VQLISPVALE EGMRFAIREG GRTIGAGTIS KIIA
