EFTU_CYAPA
ID EFTU_CYAPA Reviewed; 409 AA.
AC P17245;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Elongation factor Tu, cyanelle;
DE Short=EF-Tu;
GN Name=tufA;
OS Cyanophora paradoxa.
OG Plastid; Cyanelle.
OC Eukaryota; Glaucocystophyceae; Cyanophoraceae; Cyanophora.
OX NCBI_TaxID=2762;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=UTEX LB 555 / Pringsheim;
RX PubMed=2129337; DOI=10.1007/bf00017831;
RA Kraus M., Goetz M., Loeffelhardt W.;
RT "The cyanelle str operon from Cyanophora paradoxa: sequence analysis and
RT phylogenetic implications.";
RL Plant Mol. Biol. 15:561-573(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UTEX LB 555 / Pringsheim;
RA Stirewalt V.L., Michalowski C.B., Loeffelhardt W., Bohnert H.J.,
RA Bryant D.A.;
RT "Nucleotide sequence of the cyanelle DNA from Cyanophora paradoxa.";
RL Plant Mol. Biol. Rep. 13:327-332(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UTEX LB 555 / Pringsheim;
RA Loeffelhardt W., Stirewalt V.L., Michalowski C.B., Annarella M.,
RA Farley J.Y., Schluchter W.M., Chung S., Newmann-Spallart C., Steiner J.M.,
RA Jakowitsch J., Bohnert H.J., Bryant D.A.;
RT "The complete sequence of the cyanelle genome of Cyanophora paradoxa: the
RT genetic complexity of a primitive plastid.";
RL (In) Schenk H.E.A., Herrmann R., Jeon K.W., Mueller N.E., Schwemmler W.
RL (eds.);
RL Eukaryotism and symbiosis, pp.40-48, Springer-Verlag, Heidelberg (1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 357-409.
RX PubMed=1901820; DOI=10.1016/0378-1119(91)90170-g;
RA Bryant D.A., Schluchter W.M., Stirewalt V.L.;
RT "Ferredoxin and ribosomal protein S10 are encoded on the cyanelle genome of
RT Cyanophora paradoxa.";
RL Gene 98:169-175(1991).
CC -!- FUNCTION: This protein promotes the GTP-dependent binding of aminoacyl-
CC tRNA to the A-site of ribosomes during protein biosynthesis.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, cyanelle.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC subfamily. {ECO:0000305}.
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DR EMBL; X52497; CAA36740.1; -; Genomic_DNA.
DR EMBL; U30821; AAA81238.1; -; Genomic_DNA.
DR EMBL; M35206; AAA31701.1; -; Genomic_DNA.
DR PIR; T06895; EFKTT.
DR RefSeq; NP_043207.1; NC_001675.1.
DR AlphaFoldDB; P17245; -.
DR SMR; P17245; -.
DR GeneID; 801571; -.
DR GO; GO:0009842; C:cyanelle; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR CDD; cd01884; EF_Tu; 1.
DR CDD; cd03697; EFTU_II; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00118_B; EF_Tu_B; 1.
DR InterPro; IPR041709; EF-Tu_GTP-bd.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR033720; EFTU_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004541; Transl_elong_EFTu/EF1A_bac/org.
DR InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF03143; GTP_EFTU_D3; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF50465; SSF50465; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00485; EF-Tu; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 3: Inferred from homology;
KW Cyanelle; Elongation factor; GTP-binding; Nucleotide-binding; Plastid;
KW Protein biosynthesis.
FT CHAIN 1..409
FT /note="Elongation factor Tu, cyanelle"
FT /id="PRO_0000091455"
FT DOMAIN 10..214
FT /note="tr-type G"
FT REGION 19..26
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 60..64
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 81..84
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 136..139
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 174..176
FT /note="G5"
FT /evidence="ECO:0000250"
FT BINDING 19..26
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 81..85
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 136..139
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT CONFLICT 391
FT /note="A -> R (in Ref. 1; CAA36740)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 409 AA; 44538 MW; 5DCE5B37E0FE9759 CRC64;
MARQKFDGNK PHVNIGTIGH VDHGKTTLTA AITTALASQG KGKARKYDEI DAAPEEKARG
ITINTAHVEY ETEKRHYAHV DCPGHADYVK NMITGAAQMD GAILVVSAAD GPMPQTREHI
LLAKQVGVPN MVVFLNKEDQ IDDADLLELV ELEVRELLSK YDFPGDQIPF VSGSALLALE
SLSSNPKLMR GEDKWVDKIL ALMDAVDEYI PTPERPIDKS FLMAIEDVFS ITGRGTVATG
RIERGAIKVG ETVELVGLKD TKSTTVTGLE MFQKTLEEGM AGDNIGILLR GVQKTDIERG
MVLAKPGSIT PHTQFESEVY VLTKDEGGRH TPFFSGYRPQ FYVRTTDVTG SIDAFTADDG
SNAEMVMPGD RIKMTVSLVH PIAIEQGMRF AIREGGRTIG AGVVSKILK
