ID   AFLAP_LYOSH             Reviewed;          30 AA.
AC   C0HM10;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 1.
DT   03-AUG-2022, entry version 2.
DE   RecName: Full=Antifungal protein Lap {ECO:0000303|PubMed:11556814};
DE   Flags: Fragment;
OS   Lyophyllum shimeji (Hon-shimeji) (Tricholoma shimeji).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Lyophyllaceae; Lyophyllum.
OX   NCBI_TaxID=47721 {ECO:0000303|PubMed:11556814};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, AND FUNCTION.
RC   TISSUE=Fruiting body {ECO:0000303|PubMed:11556814};
RX   PubMed=11556814; DOI=10.1006/abbi.2001.2506;
RA   Lam S.K., Ng T.B.;
RT   "First simultaneous isolation of a ribosome inactivating protein and an
RT   antifungal protein from a mushroom (Lyophyllum shimeji) together with
RT   evidence for synergism of their antifungal effects.";
RL   Arch. Biochem. Biophys. 393:271-280(2001).
CC   -!- FUNCTION: Displays antifungal activity against M.arachidicola and
CC       P.piricola, but not against R.solani, C.gossypii and C.comatus
CC       (PubMed:11556814). Inhibits mycelial growth in P.piricola with an
CC       IC(50) of 70 nM (PubMed:11556814). Displays very low cell-free
CC       translation inhibitory activity in a rabbit reticulocyte lysate system
CC       (IC(50)=70 uM) but is able to inhibit HIV-1 reverse transcriptase
CC       activity (IC(50)=5.2 nM) (PubMed:11556814).
CC       {ECO:0000269|PubMed:11556814}.
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DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Fungicide.
FT   CHAIN           1..30
FT                   /note="Antifungal protein Lap"
FT                   /id="PRO_0000455338"
FT   NON_TER         30
FT                   /evidence="ECO:0000303|PubMed:11556814"
SQ   SEQUENCE   30 AA;  3026 MW;  0F3B0CE7DF6C9B56 CRC64;
     AGTEIVTCYN AGTKVPRGPS AXGGAIDFFN