ID   AFLR_ASPFN              Reviewed;         444 AA.
AC   P41765; B8NHZ9;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   14-APR-2009, sequence version 2.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Aflatoxin biosynthesis regulatory protein;
GN   Name=aflR; Synonyms=afl-2, afl2, apa-2; ORFNames=AFLA_139360;
OS   Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS   / JCM 12722 / SRRC 167).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=332952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8074521; DOI=10.1128/aem.60.7.2408-2414.1994;
RA   Woloshuk C.P., Foutz K.R., Brewer J.F., Bhatnagar D., Cleveland T.E.,
RA   Payne G.A.;
RT   "Molecular characterization of aflR, a regulatory locus for aflatoxin
RT   biosynthesis.";
RL   Appl. Environ. Microbiol. 60:2408-2414(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC   167;
RX   PubMed=25883274; DOI=10.1128/genomea.00168-15;
RA   Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA   Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT   "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT   aflatoxin contamination of food and feed.";
RL   Genome Announc. 3:E0016815-E0016815(2015).
CC   -!- FUNCTION: Involved in the regulation of aflatoxin biosynthesis.
CC       Required for the expression of the NOR1 and VER1 genes.
CC   -!- PATHWAY: Mycotoxin biosynthesis; aflatoxin biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA57137.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=AAA57138.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; L32576; AAA57137.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; L32577; AAA57138.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; EQ963478; EED51170.1; -; Genomic_DNA.
DR   RefSeq; XP_002379946.1; XM_002379905.1.
DR   AlphaFoldDB; P41765; -.
DR   SMR; P41765; -.
DR   PRIDE; P41765; -.
DR   EnsemblFungi; EED51170; EED51170; AFLA_139360.
DR   VEuPathDB; FungiDB:AFLA_139360; -.
DR   HOGENOM; CLU_031656_1_0_1; -.
DR   OMA; ACARCIE; -.
DR   UniPathway; UPA00287; -.
DR   Proteomes; UP000001875; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0045122; P:aflatoxin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR013700; AflR.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF08493; AflR; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   4: Predicted;
KW   DNA-binding; Metal-binding; Nucleus; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..444
FT                   /note="Aflatoxin biosynthesis regulatory protein"
FT                   /id="PRO_0000114932"
FT   DNA_BIND        29..56
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          64..174
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        74..121
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        132..154
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   444 AA;  47539 MW;  0FBA53A8BE7E2252 CRC64;
     MVDHISPRAS PGPIRSSQTR RARKLRDSCT SCASSKVRCT KEKPACARCI ERGLACQYMV
     SKRMGRNPRA PSPLDSTRRP SESLPSARSE QGLPAHNTYS TPHAHTQAHT HAHSHPQPHP
     QSHPQSNQPP HALPTPNGSS SVSAIFSHQS PPPPVETQGL GGDLAGQEQS TLSSLTVDSE
     FGGSLQSMEH GNHVDFLAES TGSLFDAFLE VGTPMIDPFL ESAPLPPFQA RYCCFSLALQ
     TLTHLFPHAP LGCQLRLTDG EDSSCNLMTT DMVISGNKRA TDAVRKILGC SCAQDGYLLS
     MVVLIVLKVL AWYAAAAGTQ CTSTAAGGET NSGSCSNSPA TVSSGCLTEE RVLHLPSMMG
     EDCVDEEDQP RVAAQLVLSE LHRVQSLVNL LAKRLQEGGD DAAGIPAHHP ASPFSLLGFS
     GLEANLRHRL RAVSSDIIDY LHRE