ID   AFLR_DOTSN              Reviewed;         479 AA.
AC   M2YIY2;
DT   28-FEB-2018, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2013, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Dothistromin biosynthesis regulatory protein aflR {ECO:0000303|PubMed:23207690};
GN   Name=aflR {ECO:0000303|PubMed:23207690}; ORFNames=DOTSEDRAFT_75566;
OS   Dothistroma septosporum (strain NZE10 / CBS 128990) (Red band needle blight
OS   fungus) (Mycosphaerella pini).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Dothistroma.
OX   NCBI_TaxID=675120;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NZE10 / CBS 128990;
RX   PubMed=23209441; DOI=10.1371/journal.pgen.1003088;
RA   de Wit P.J.G.M., van der Burgt A., Oekmen B., Stergiopoulos I.,
RA   Abd-Elsalam K.A., Aerts A.L., Bahkali A.H., Beenen H.G., Chettri P.,
RA   Cox M.P., Datema E., de Vries R.P., Dhillon B., Ganley A.R.,
RA   Griffiths S.A., Guo Y., Hamelin R.C., Henrissat B., Kabir M.S.,
RA   Jashni M.K., Kema G., Klaubauf S., Lapidus A., Levasseur A., Lindquist E.,
RA   Mehrabi R., Ohm R.A., Owen T.J., Salamov A., Schwelm A., Schijlen E.,
RA   Sun H., van den Burg H.A., van Ham R.C.H.J., Zhang S., Goodwin S.B.,
RA   Grigoriev I.V., Collemare J., Bradshaw R.E.;
RT   "The genomes of the fungal plant pathogens Cladosporium fulvum and
RT   Dothistroma septosporum reveal adaptation to different hosts and lifestyles
RT   but also signatures of common ancestry.";
RL   PLoS Genet. 8:E1003088-E1003088(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NZE10 / CBS 128990;
RX   PubMed=23236275; DOI=10.1371/journal.ppat.1003037;
RA   Ohm R.A., Feau N., Henrissat B., Schoch C.L., Horwitz B.A., Barry K.W.,
RA   Condon B.J., Copeland A.C., Dhillon B., Glaser F., Hesse C.N., Kosti I.,
RA   LaButti K., Lindquist E.A., Lucas S., Salamov A.A., Bradshaw R.E.,
RA   Ciuffetti L., Hamelin R.C., Kema G.H.J., Lawrence C., Scott J.A.,
RA   Spatafora J.W., Turgeon B.G., de Wit P.J.G.M., Zhong S., Goodwin S.B.,
RA   Grigoriev I.V.;
RT   "Diverse lifestyles and strategies of plant pathogenesis encoded in the
RT   genomes of eighteen Dothideomycetes fungi.";
RL   PLoS Pathog. 8:E1003037-E1003037(2012).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=23207690; DOI=10.1016/j.fgb.2012.11.006;
RA   Chettri P., Ehrlich K.C., Cary J.W., Collemare J., Cox M.P.,
RA   Griffiths S.A., Olson M.A., de Wit P.J., Bradshaw R.E.;
RT   "Dothistromin genes at multiple separate loci are regulated by AflR.";
RL   Fungal Genet. Biol. 51:12-20(2013).
CC   -!- FUNCTION: Transcription factor involved in regulation of the
CC       dothistromin biosynthesis fragmented gene cluster (PubMed:23207690).
CC       {ECO:0000269|PubMed:23207690}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- DISRUPTION PHENOTYPE: Leads to drastically reduced, but still
CC       detectable production of dothistromin (PubMed:23207690).
CC       {ECO:0000269|PubMed:23207690}.
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DR   EMBL; KB446546; EME38895.1; -; Genomic_DNA.
DR   AlphaFoldDB; M2YIY2; -.
DR   SMR; M2YIY2; -.
DR   EnsemblFungi; EME38895; EME38895; DOTSEDRAFT_75566.
DR   eggNOG; ENOG502SUW5; Eukaryota.
DR   HOGENOM; CLU_031656_1_0_1; -.
DR   OMA; ACARCIE; -.
DR   OrthoDB; 1396569at2759; -.
DR   Proteomes; UP000016933; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0045122; P:aflatoxin biosynthetic process; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR013700; AflR.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF08493; AflR; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..479
FT                   /note="Dothistromin biosynthesis regulatory protein aflR"
FT                   /id="PRO_0000443470"
FT   DNA_BIND        32..59
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          63..100
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          349..382
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        64..96
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   479 AA;  51937 MW;  F17505ED9AFA9710 CRC64;
     MPESRGSESS GSTSRRASGT QHIATPKLKD SCTACATSKV KCSKDKPTCA RCTRRGLTCD
     YGLSKRTGRT SHATAQKQQQ QRQQQQQSQQ EAADRRVSQS AVPVANMLPD VDEFISPMSL
     QESSILSPTL MPDLSTSGSQ AGLDQWNSDL WSTMFAPNEP SSNNTPANTM QTHGGNGNDI
     DHLFNDLTSY SMLGEPTDTA MSNQHTHTFS DTSSSISPFD QLTSVSDMGS QDFSNIDLSM
     TNCTPAIQQS VEPANCCLTV ALGFMTQLCA TASSSCTMPG SHNGNTTLPT IDSVITENRQ
     IVDQIVKILE CPCSHDEYLL TIVHLVVFKV MAWYAAAARE KPSLAEEINW TDQQSGRPRS
     RTHSEEVLRF PPSIDGYSSD GSDNGRMAAQ LVLSELHRVQ RLVNLLSQRL EGVRLRNHVA
     SSGSSSSLES IGEDSVVGVS LSATAGSPLS SPTFDQLEAD LRKRLRAVSF ETIDVLRRS