ID   AFLR_EMENI              Reviewed;         433 AA.
AC   P52957; C8VDU4; Q5AV60;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2007, sequence version 2.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=Sterigmatocystin biosynthesis regulatory protein {ECO:0000303|PubMed:8643646};
DE   AltName: Full=Sterigmatocystin biosynthesis cluster protein aflR {ECO:0000303|PubMed:8643646};
GN   Name=aflR {ECO:0000303|PubMed:8643646}; ORFNames=AN7820;
OS   Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS   M139) (Aspergillus nidulans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=227321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=FGSC 26;
RX   PubMed=8643646; DOI=10.1073/pnas.93.4.1418;
RA   Brown D.W., Yu J.-H., Kelkar H.S., Fernandes M., Nesbitt T.C., Keller N.P.,
RA   Adams T.H., Leonard T.J.;
RT   "Twenty-five coregulated transcripts define a sterigmatocystin gene cluster
RT   in Aspergillus nidulans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:1418-1422(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=8662194; DOI=10.1007/bf02426959;
RA   Yu J.-H., Butchko R.A.E., Fernandes M., Keller N.P., Leonard T.J.,
RA   Adams T.H.;
RT   "Conservation of structure and function of the aflatoxin regulatory gene
RT   aflR from Aspergillus nidulans and A. flavus.";
RL   Curr. Genet. 29:549-555(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=16372000; DOI=10.1038/nature04341;
RA   Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA   Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA   Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA   Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA   Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA   Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA   Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA   Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA   Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT   "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT   fumigatus and A. oryzae.";
RL   Nature 438:1105-1115(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA   Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA   Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA   Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA   Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA   Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA   Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA   Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA   van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA   Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA   Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA   Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA   Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA   Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA   van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA   Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA   Oliver S.G., Turner G.;
RT   "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT   effort.";
RL   Fungal Genet. Biol. 46:S2-13(2009).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=9680223; DOI=10.1046/j.1365-2958.1998.00907.x;
RA   Fernandes M., Keller N.P., Adams T.H.;
RT   "Sequence-specific binding by Aspergillus nidulans aflR, a C6 zinc cluster
RT   protein regulating mycotoxin biosynthesis.";
RL   Mol. Microbiol. 28:1355-1365(1998).
CC   -!- FUNCTION: Transcription factor; part of the gene cluster that mediates
CC       the biosynthesis of sterigmatocystin (ST), a polyketide-derived
CC       furanocoumarin which is part of the most toxic and carcinogenic
CC       compounds among the known mycotoxins (PubMed:8643646, PubMed:8662194).
CC       Binds to the palindromic sequence 5'-TCG(N5)CGA-3'found in the promoter
CC       regions of several sterigmatocystin cluster genes to induce their
CC       expression (PubMed:9680223). {ECO:0000269|PubMed:8643646,
CC       ECO:0000269|PubMed:8662194, ECO:0000269|PubMed:9680223}.
CC   -!- PATHWAY: Mycotoxin biosynthesis; sterigmatocystin biosynthesis
CC       [regulation]. {ECO:0000269|PubMed:9680223}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- DISRUPTION PHENOTYPE: Imeairs expression of the sterigmatocystin
CC       cluster genes. {ECO:0000269|PubMed:9680223}.
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DR   EMBL; U34740; AAC49195.1; -; Genomic_DNA.
DR   EMBL; AACD01000132; EAA61608.1; -; Genomic_DNA.
DR   EMBL; BN001304; CBF80174.1; -; Genomic_DNA.
DR   PIR; S69999; S69999.
DR   RefSeq; XP_681089.1; XM_675997.1.
DR   AlphaFoldDB; P52957; -.
DR   SMR; P52957; -.
DR   STRING; 162425.CADANIAP00000963; -.
DR   EnsemblFungi; CBF80174; CBF80174; ANIA_07820.
DR   EnsemblFungi; EAA61608; EAA61608; AN7820.2.
DR   GeneID; 2869641; -.
DR   KEGG; ani:AN7820.2; -.
DR   VEuPathDB; FungiDB:AN7820; -.
DR   eggNOG; ENOG502SUW5; Eukaryota.
DR   HOGENOM; CLU_031656_1_0_1; -.
DR   InParanoid; P52957; -.
DR   OMA; ACARCIE; -.
DR   OrthoDB; 1396569at2759; -.
DR   UniPathway; UPA00377; -.
DR   Proteomes; UP000000560; Chromosome IV.
DR   Proteomes; UP000005890; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:AspGD.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:AspGD.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0045122; P:aflatoxin biosynthetic process; IEA:InterPro.
DR   GO; GO:0036184; P:asperthecin biosynthetic process; IGI:AspGD.
DR   GO; GO:1900381; P:positive regulation of asperthecin biosynthetic process; IGI:AspGD.
DR   GO; GO:1900378; P:positive regulation of secondary metabolite biosynthetic process; IMP:AspGD.
DR   GO; GO:0010914; P:positive regulation of sterigmatocystin biosynthetic process; IMP:AspGD.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:AspGD.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:AspGD.
DR   GO; GO:0045461; P:sterigmatocystin biosynthetic process; IMP:AspGD.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR013700; AflR.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF08493; AflR; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..433
FT                   /note="Sterigmatocystin biosynthesis regulatory protein"
FT                   /id="PRO_0000114934"
FT   DNA_BIND        28..55
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..23
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          73..102
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          378..404
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        75..102
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        380..404
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        340
FT                   /note="S -> T (in Ref. 1; AAC49195)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   433 AA;  46566 MW;  C9B8A0F83799472D CRC64;
     MEPPAISQQS TPTAPGGTQG TRKLRESCIS CSRSKVKCNK EKPTCSRCVR RGLPCEYMVS
     RRTGRTRVIG VEQPKTAPSP TTPTNTTAAT TATKAGPPVT TDSAVHTPVI TTAPSPKPVQ
     IQSPPAEPDL WGAILSPNTS TSTDLSSLLS VNTNFSQLFA SLSPSLLEGM DGMDAEMHAP
     ELGALSVADP SSSMMQGLEA PNAAQPPSSN TTSHSYCLSI CLDTLMRLFP NAGANCERPG
     HESNPGKLFT IESVIEDNKQ ILDTAQTILA CRCAEDEYVV TLVSLIVFKV LGWYVAAARD
     RSSDPGREED FNWSTAQDSR RGSVSSFEEQ VLHLPTVVGS YCIDGHHQSR MAAQLVLSEL
     YRVQRLVTQV SRRLESIRRR SSSSSSSASS NTTDSDGGMS TPLSSTTLVH LEDDLRKRLR
     AVSSETISIL RHA