AFLS_ASPPU
ID AFLS_ASPPU Reviewed; 438 AA.
AC O42716; A0A0F0HZ07;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=Aflatoxin cluster transcriptional coactivator aflS {ECO:0000305};
DE AltName: Full=Aflatoxin biosynthesis protein S {ECO:0000303|PubMed:15006741};
GN Name=aflS {ECO:0000303|PubMed:15006741}; Synonyms=aflJ;
GN ORFNames=P875_00052983;
OS Aspergillus parasiticus (strain ATCC 56775 / NRRL 5862 / SRRC 143 / SU-1).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX NCBI_TaxID=1403190;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 56775 / NRRL 5862 / SRRC 143 / SU-1;
RA Chang P.-K., Cotty P.J., Bhatnagar D., Bennett J.W., Cleveland T.E.;
RT "Overexpression of the Aspergillus parasiticus aflJ gene, in conjunction
RT with the aflR regulatory gene, elevates aflatoxin biosynthesis and affects
RT sclerotial development.";
RL Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND NOMENCLATURE.
RC STRAIN=ATCC 56775 / NRRL 5862 / SRRC 143 / SU-1;
RX PubMed=15006741; DOI=10.1128/aem.70.3.1253-1262.2004;
RA Yu J., Chang P.K., Ehrlich K.C., Cary J.W., Bhatnagar D., Cleveland T.E.,
RA Payne G.A., Linz J.E., Woloshuk C.P., Bennett J.W.;
RT "Clustered pathway genes in aflatoxin biosynthesis.";
RL Appl. Environ. Microbiol. 70:1253-1262(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 56775 / NRRL 5862 / SRRC 143 / SU-1;
RX PubMed=15094053; DOI=10.1016/s0014-5793(04)00327-8;
RA Yu J., Bhatnagar D., Cleveland T.E.;
RT "Completed sequence of aflatoxin pathway gene cluster in Aspergillus
RT parasiticus.";
RL FEBS Lett. 564:126-130(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 56775 / NRRL 5862 / SRRC 143 / SU-1;
RA Yu J., Fedorova N., Yin Y., Losada L., Zafar N., Taujale R., Ehrlich K.C.,
RA Bhatnagar D., Cleveland T.E., Bennett J.W., Nierman W.C.;
RT "Draft genome sequence of Aspergillus parasiticus SU-1.";
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION.
RX PubMed=11913765; DOI=10.1023/a:1015211915310;
RA Chang P.K., Bennett J.W., Cotty P.J.;
RT "Association of aflatoxin biosynthesis and sclerotial development in
RT Aspergillus parasiticus.";
RL Mycopathologia 153:41-48(2002).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH AFLR.
RX PubMed=12655397; DOI=10.1007/s00438-003-0809-3;
RA Chang P.K.;
RT "The Aspergillus parasiticus protein AFLJ interacts with the aflatoxin
RT pathway-specific regulator AFLR.";
RL Mol. Genet. Genomics 268:711-719(2003).
RN [7]
RP INDUCTION.
RX PubMed=15294809; DOI=10.1128/aem.70.8.4733-4739.2004;
RA Calvo A.M., Bok J., Brooks W., Keller N.P.;
RT "veA is required for toxin and sclerotial production in Aspergillus
RT parasiticus.";
RL Appl. Environ. Microbiol. 70:4733-4739(2004).
RN [8]
RP FUNCTION, AND INTERACTION WITH AFLR.
RX PubMed=15054098; DOI=10.1074/jbc.m400075200;
RA Roze L.V., Miller M.J., Rarick M., Mahanti N., Linz J.E.;
RT "A novel cAMP-response element, CRE1, modulates expression of nor-1 in
RT Aspergillus parasiticus.";
RL J. Biol. Chem. 279:27428-27439(2004).
RN [9]
RP FUNCTION.
RX PubMed=23605486; DOI=10.1007/s12550-010-0062-7;
RA Schmidt-Heydt M., Ruefer C.E., Abdel-Hadi A., Magan N., Geisen R.;
RT "The production of aflatoxin B1 or G 1 by Aspergillus parasiticus at
RT various combinations of temperature and water activity is related to the
RT ratio of aflS to aflR expression.";
RL Mycotoxin Res. 26:241-246(2010).
RN [10]
RP FUNCTION, INTERACTION WITH AFLR, AND SUBCELLULAR LOCATION.
RX PubMed=23342682; DOI=10.3390/toxins4121582;
RA Ehrlich K.C., Mack B.M., Wei Q., Li P., Roze L.V., Dazzo F., Cary J.W.,
RA Bhatnagar D., Linz J.E.;
RT "Association with AflR in endosomes reveals new functions for AflJ in
RT aflatoxin biosynthesis.";
RL Toxins 4:1582-1600(2012).
RN [11]
RP INDUCTION.
RX PubMed=25741015; DOI=10.1099/mic.0.000070;
RA Verheecke C., Liboz T., Anson P., Diaz R., Mathieu F.;
RT "Reduction of aflatoxin production by Aspergillus flavus and Aspergillus
RT parasiticus in interaction with Streptomyces.";
RL Microbiology 161:967-972(2015).
CC -!- FUNCTION: Transcription coactivator involved in regulation of the
CC aflatoxin biosynthesis gene cluster with aflR (PubMed:15006741,
CC PubMed:11913765, PubMed:12655397, PubMed:15054098, PubMed:23605486).
CC The ratio of the expression data between aflS:aflR plays a crucial role
CC in the regulation of aflatoxins production (PubMed:23605486). A high
CC ratio, produced at a range between 17 and 30 degrees Celsius,
CC corresponds with the production profile of aflatoxin G1 biosynthesis
CC (PubMed:23605486). A low ratio, produced over 30 degrees Celsius, is
CC related to aflatoxin B1 biosynthesis (PubMed:23605486). AflJ may act in
CC aflR transport to or from the nucleus, thus controlling the
CC availability of aflR for transcriptional activation of aflatoxin
CC biosynthesis cluster genes (PubMed:23342682). AflJ may also assist in
CC directing endosomes to the cytoplasmic membrane for aflatoxin export
CC (PubMed:23342682). {ECO:0000269|PubMed:11913765,
CC ECO:0000269|PubMed:12655397, ECO:0000269|PubMed:15006741,
CC ECO:0000269|PubMed:15054098, ECO:0000269|PubMed:23342682,
CC ECO:0000269|PubMed:23605486}.
CC -!- SUBUNIT: Interacts with aflR (PubMed:12655397, PubMed:15054098,
CC PubMed:23342682). {ECO:0000269|PubMed:12655397,
CC ECO:0000269|PubMed:15054098, ECO:0000269|PubMed:23342682}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23342682}. Endosome
CC {ECO:0000269|PubMed:23342682}.
CC -!- INDUCTION: Expression is positively regulated by the developmental and
CC secondary metabolism regulator veA (PubMed:15294809). Expression is
CC repressed during Streptomyces-Aspergillus interactions
CC (PubMed:25741015). {ECO:0000269|PubMed:15294809,
CC ECO:0000269|PubMed:25741015}.
CC -!- DISRUPTION PHENOTYPE: Leads to significant decrease in the transcript
CC levels of the genes for early (aflC and aflD), middle (aflM) and later
CC (aflP) steps in the aflatoxin biosynthetic pathway (PubMed:12655397).
CC {ECO:0000269|PubMed:12655397}.
CC -!- SEQUENCE CAUTION:
CC Sequence=KJK60754.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF002660; AAB94411.2; -; Genomic_DNA.
DR EMBL; AY371490; AAS66019.1; -; Genomic_DNA.
DR EMBL; JZEE01000729; KJK60754.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; O42716; -.
DR SMR; O42716; -.
DR EnsemblFungi; KJK60754; KJK60754; P875_00052983.
DR Proteomes; UP000033540; Unassembled WGS sequence.
DR GO; GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:1900179; P:positive regulation of aflatoxin biosynthetic process; IDA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR SUPFAM; SSF46785; SSF46785; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Endosome; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..438
FT /note="Aflatoxin cluster transcriptional coactivator aflS"
FT /id="PRO_0000438339"
FT DOMAIN 65..134
FT /note="HTH iclR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00393"
FT DNA_BIND 95..114
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00393"
SQ SEQUENCE 438 AA; 47229 MW; AB645C68A789A603 CRC64;
MTLTDLETCA EEIATAARTL ARDGHSGGYS AGLPDHLRAV QRTLIANASQ VLALASQPAD
LVRQLALYNQ LLACLRWLGE FQVLACIPLD ESVPFEDVAD IAGVPECRLR RLVRPLFTIG
FLCEPSPGHV AHSVLSKQFV TQPALLDAIL FMSETLAPSA SAMGTQTRRF GASEQAGDSA
WNMAVGSDSP FAACLQQRPK VKRQLGAYLS YVSSAIDAGV EDTLTRMNWQ NLGMATVVHV
GAQSPSLVVA LAPQFPSLRF LVQTEAKAES GGHQPCLDNH GIPALKLASI PLHLRARITW
GTRLSTATQP VLDAAVYLIS IPFPSPQSPA MEITMRVAQA LKAHVEVLRN NSDARLILTL
PMSSATRSMD AAARAAVSLS DLSLLQLTNG GSLNMGEIRD LLRSRSDGLV VMREVRSPTN
AVIAFEIQYR VDNDDNRY
