3L24_NAJNA
ID 3L24_NAJNA Reviewed; 71 AA.
AC P25672;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Long neurotoxin 4;
DE AltName: Full=Toxin D {ECO:0000303|PubMed:7326259};
OS Naja naja (Indian cobra).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX NCBI_TaxID=35670;
RN [1]
RP PROTEIN SEQUENCE, TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RX PubMed=7326259; DOI=10.1016/0005-2795(81)90125-2;
RA Ohta M., Sasaki T., Hayashi K.;
RT "The amino acid sequence of toxin D isolated from the venom of Indian cobra
RT (Naja naja).";
RL Biochim. Biophys. Acta 671:123-128(1981).
CC -!- FUNCTION: Binds with high affinity to muscular (alpha-1/CHRNA1) and
CC neuronal (alpha-7/CHRNA7) nicotinic acetylcholine receptor (nAChR) and
CC inhibits acetylcholine from binding to the receptor, thereby impairing
CC neuromuscular and neuronal transmission.
CC {ECO:0000250|UniProtKB:P60615}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:7326259}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:7326259}.
CC -!- TOXIC DOSE: LD(50) is 0.22 mg/kg by subcutaneous injection.
CC {ECO:0000269|PubMed:7326259}.
CC -!- SIMILARITY: Belongs to the snake three-finger toxin family. Long-chain
CC subfamily. Type II alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR AlphaFoldDB; P25672; -.
DR SMR; P25672; -.
DR Proteomes; UP000694559; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR CDD; cd00206; snake_toxin; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR003571; Snake_3FTx.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR018354; Snake_toxin_con_site.
DR SUPFAM; SSF57302; SSF57302; 1.
DR PROSITE; PS00272; SNAKE_TOXIN; 1.
PE 1: Evidence at protein level;
KW Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW Postsynaptic neurotoxin; Reference proteome; Secreted; Toxin.
FT CHAIN 1..71
FT /note="Long neurotoxin 4"
FT /evidence="ECO:0000269|PubMed:7326259"
FT /id="PRO_0000093550"
FT DISULFID 3..20
FT /evidence="ECO:0000250|UniProtKB:P25671"
FT DISULFID 14..41
FT /evidence="ECO:0000250|UniProtKB:P25671"
FT DISULFID 26..30
FT /evidence="ECO:0000250|UniProtKB:P25671"
FT DISULFID 45..56
FT /evidence="ECO:0000250|UniProtKB:P25671"
FT DISULFID 57..62
FT /evidence="ECO:0000250|UniProtKB:P25671"
SQ SEQUENCE 71 AA; 7889 MW; 66F4E0686AD55813 CRC64;
IRCFITPDIT SKDCPNGHVC YTKTWCDGFC RIRGERVDLG CAATCPTVKT GVDIQCCSTD
DCDPFPTRKR P