AFOA_EMENI
ID AFOA_EMENI Reviewed; 666 AA.
AC Q5BEK1; C8VTY4;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=Asperfuranone cluster transcription factor afoA {ECO:0000303|PubMed:19199437};
DE AltName: Full=Asperfuranone biosynthesis protein A {ECO:0000303|PubMed:19199437};
GN Name=afoA {ECO:0000303|PubMed:19199437}; ORFNames=AN1029;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19199437; DOI=10.1021/ja8088185;
RA Chiang Y.M., Szewczyk E., Davidson A.D., Keller N., Oakley B.R., Wang C.C.;
RT "A gene cluster containing two fungal polyketide synthases encodes the
RT biosynthetic pathway for a polyketide, asperfuranone, in Aspergillus
RT nidulans.";
RL J. Am. Chem. Soc. 131:2965-2970(2009).
RN [4]
RP INDUCTION.
RX PubMed=20952652; DOI=10.1128/aem.00683-10;
RA Bergmann S., Funk A.N., Scherlach K., Schroeckh V., Shelest E., Horn U.,
RA Hertweck C., Brakhage A.A.;
RT "Activation of a silent fungal polyketide biosynthesis pathway through
RT regulatory cross talk with a cryptic nonribosomal peptide synthetase gene
RT cluster.";
RL Appl. Environ. Microbiol. 76:8143-8149(2010).
RN [5]
RP BIOTECHNOLOGY.
RX PubMed=20148857; DOI=10.1111/j.1742-7843.2010.00545.x;
RA Wang C.C., Chiang Y.M., Praseuth M.B., Kuo P.L., Liang H.L., Hsu Y.L.;
RT "Asperfuranone from Aspergillus nidulans inhibits proliferation of human
RT non-small cell lung cancer A549 cells via blocking cell cycle progression
RT and inducing apoptosis.";
RL Basic Clin. Pharmacol. Toxicol. 107:583-589(2010).
CC -!- FUNCTION: Transcription factor that regulates the expression of the
CC gene cluster that mediates the biosynthesis of asperfuranone, a
CC probable antitumor agent (PubMed:19199437).
CC {ECO:0000269|PubMed:19199437}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- INDUCTION: Expression is positively regulated by the secondary
CC metabolism cross-pathway regulator scpR (PubMed:20952652).
CC {ECO:0000269|PubMed:20952652}.
CC -!- DISRUPTION PHENOTYPE: Leads to decreased production of asperfuranone
CC (PubMed:19199437). {ECO:0000269|PubMed:19199437}.
CC -!- BIOTECHNOLOGY: Asperfuranone provides anti-proliferative activity in
CC human non-small cell lung cancer cells (PubMed:20148857).
CC {ECO:0000269|PubMed:20148857}.
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DR EMBL; BN001308; CBF88304.1; -; Genomic_DNA.
DR EMBL; AACD01000015; EAA65597.1; -; Genomic_DNA.
DR RefSeq; XP_658633.1; XM_653541.1.
DR AlphaFoldDB; Q5BEK1; -.
DR STRING; 162425.CADANIAP00001618; -.
DR EnsemblFungi; CBF88304; CBF88304; ANIA_01029.
DR EnsemblFungi; EAA65597; EAA65597; AN1029.2.
DR GeneID; 2876808; -.
DR KEGG; ani:AN1029.2; -.
DR eggNOG; ENOG502SH49; Eukaryota.
DR HOGENOM; CLU_011017_3_1_1; -.
DR InParanoid; Q5BEK1; -.
DR OMA; EWPLTLQ; -.
DR OrthoDB; 455010at2759; -.
DR Proteomes; UP000000560; Chromosome VIII.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR007219; Transcription_factor_dom_fun.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF04082; Fungal_trans; 1.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00906; Fungal_trans; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..666
FT /note="Asperfuranone cluster transcription factor afoA"
FT /id="PRO_0000436369"
FT DNA_BIND 16..43
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 184..206
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 347..373
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 350..372
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 666 AA; 74049 MW; E24B60324F977D26 CRC64;
MACPTRRGRQ QPGFACEECR RRKARCDRVR PKCGFCTENE LQCVFVDKRQ QRGPIKGQIT
SMQSQLATLR WQLDRYLRHR PPPSITMAGE LDEPPADIQT MLDDFDVQVA ALKQDATATT
TMSTSTALMP APAISSKDAA PAGAGLSWPD PTWLDRQWQD VSSTSLVPPS DLTVSSATTL
TDPLSFDLLN ETPPPPSTTT TTSTTRRDSC TKVMLTDLIR AELDQLYFDR VHAFCPIIHR
RRYFARVARD SHTPAQACLQ FAMRTLAAAM SAHCHLSEHL YAETKALLET HSQTPATPRD
KVPLEHIQAW LLLSHYELLR IGVHQAMLTA GRAFRLVQMA RLSELDAGSD RQLSPPSSSP
PSSLTLSPSG ENAENFVDAE EGRRTFWLAY CFDRLLCLQN EWPLTLQEEM ILTRLPSLEH
NYQNNLPART PFLTEAMAQT GQSTMSPFAE CIIMATLHGR CMTHRRFYAN SNSTASGSEF
ESGAATRDFC IRQNWLSNAV DRRVQMLQQV SSPAVDSDPM LLFTQTLGYR ATMHLSDTVQ
QVSWRALASS PVDQQLLSPG ATMSLSAAAY HQMASHAAGE IVRLAKAVPS LSPFKAHPFL
PDTLACAATF LSTGSPDPTG GEGVQHLLRV LSELRDTHSL ARDYLQGLSV QTQDEDHRQD
TRWYCT
