AFP1_ARATH
ID AFP1_ARATH Reviewed; 345 AA.
AC Q9LQ98; Q8L8S9; Q9C980;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Ninja-family protein AFP1;
DE AltName: Full=ABI five-binding protein 1;
DE Short=ABI5-binding protein 1;
GN Name=AFP1; Synonyms=AFP; OrderedLocusNames=At1g69260;
GN ORFNames=F23O10.16, F4N2.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION, INTERACTION
RP WITH ABI5/DPBF1, AND DISRUPTION PHENOTYPE.
RX PubMed=12569131; DOI=10.1101/gad.1055803;
RA Lopez-Molina L., Mongrand S., Kinoshita N., Chua N.-H.;
RT "AFP is a novel negative regulator of ABA signaling that promotes ABI5
RT protein degradation.";
RL Genes Dev. 17:410-418(2003).
RN [6]
RP GENE FAMILY, NOMENCLATURE, SUBUNIT, INTERACTION WITH ABI5/DPBF1; DPBF2;
RP AREB3/DPBF3; ABF1; ABF3/DPBF5 AND ABF4/AREB2, INDUCTION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=18484180; DOI=10.1007/s11103-008-9344-2;
RA Garcia M.E., Lynch T.J., Peeters J., Snowden C., Finkelstein R.R.;
RT "A small plant-specific protein family of ABI five binding proteins (AFPs)
RT regulates stress response in germinating Arabidopsis seeds and seedlings.";
RL Plant Mol. Biol. 67:643-658(2008).
CC -!- FUNCTION: Acts as a negative regulator of abscisic acid (ABA) response
CC during germination through the ubiquitin-mediated proteolysis of
CC ABI5/DPBF1. {ECO:0000269|PubMed:12569131}.
CC -!- SUBUNIT: Forms a heterodimer with AFP2. Interacts with ABI5/DPBF1,
CC DPBF2, AREB3/DPBF3, ABF1, ABF3/DPBF5 and ABF4/AREB2.
CC {ECO:0000269|PubMed:12569131, ECO:0000269|PubMed:18484180}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12569131}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryo during the latest stages of
CC seed maturation. {ECO:0000269|PubMed:12569131}.
CC -!- INDUCTION: Up-regulated by abscisic acid (ABA), glucose and salt.
CC {ECO:0000269|PubMed:12569131, ECO:0000269|PubMed:18484180}.
CC -!- DISRUPTION PHENOTYPE: Exhibits slight hypersensitivity to abscisic acid
CC (ABA), salt and osmotic stress. {ECO:0000269|PubMed:12569131,
CC ECO:0000269|PubMed:18484180}.
CC -!- SIMILARITY: Belongs to the Ninja family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52486.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAM67141.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AC008262; AAF27062.1; -; Genomic_DNA.
DR EMBL; AC018364; AAG52486.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE34903.1; -; Genomic_DNA.
DR EMBL; AY136357; AAM97023.1; -; mRNA.
DR EMBL; BT000199; AAN15518.1; -; mRNA.
DR EMBL; AY088834; AAM67141.1; ALT_INIT; mRNA.
DR PIR; F96716; F96716.
DR RefSeq; NP_564956.1; NM_105593.5.
DR AlphaFoldDB; Q9LQ98; -.
DR IntAct; Q9LQ98; 10.
DR STRING; 3702.AT1G69260.1; -.
DR iPTMnet; Q9LQ98; -.
DR PaxDb; Q9LQ98; -.
DR PRIDE; Q9LQ98; -.
DR ProteomicsDB; 244843; -.
DR EnsemblPlants; AT1G69260.1; AT1G69260.1; AT1G69260.
DR GeneID; 843257; -.
DR Gramene; AT1G69260.1; AT1G69260.1; AT1G69260.
DR KEGG; ath:AT1G69260; -.
DR Araport; AT1G69260; -.
DR TAIR; locus:2026413; AT1G69260.
DR eggNOG; ENOG502QW6K; Eukaryota.
DR HOGENOM; CLU_034695_0_0_1; -.
DR InParanoid; Q9LQ98; -.
DR OMA; CVCHANF; -.
DR OrthoDB; 1080495at2759; -.
DR PhylomeDB; Q9LQ98; -.
DR PRO; PR:Q9LQ98; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LQ98; baseline and differential.
DR Genevisible; Q9LQ98; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; TAS:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0009737; P:response to abscisic acid; IBA:GO_Central.
DR InterPro; IPR032310; NINJA_B.
DR InterPro; IPR031307; Ninja_fam.
DR InterPro; IPR012463; Ninja_motif.
DR InterPro; IPR032308; TDBD.
DR PANTHER; PTHR31413; PTHR31413; 1.
DR Pfam; PF07897; EAR; 1.
DR Pfam; PF16136; NINJA_B; 1.
DR Pfam; PF16135; TDBD; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..345
FT /note="Ninja-family protein AFP1"
FT /id="PRO_0000369614"
FT REGION 114..185
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 201..256
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 149..163
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 210..256
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 84..85
FT /note="HH -> R (in Ref. 4; AAM67141)"
FT /evidence="ECO:0000305"
FT CONFLICT 190
FT /note="L -> F (in Ref. 4; AAM67141)"
FT /evidence="ECO:0000305"
FT CONFLICT 204
FT /note="G -> V (in Ref. 4; AAM67141)"
FT /evidence="ECO:0000305"
FT CONFLICT 237
FT /note="T -> N (in Ref. 4; AAM67141)"
FT /evidence="ECO:0000305"
FT CONFLICT 240
FT /note="P -> Q (in Ref. 4; AAM67141)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 345 AA; 37531 MW; 61E74572437E332A CRC64;
MAEANERSKE MARSNSCVFP RDLLQRFISN SVEGEDDDEE EDDDEIELNL GLSLGGRFGV
DKSNKLVRSS SVVVTMPLFR EDHHHHQAAA MITTKVSTET VAGATRGTGL MRTTSLPAES
EEEWRKRKEM QTLRRMAAKR RRSEKLRTGV GGGNSNNPEE AATATASRRR GRPSSGLPRW
SATANKSGLL RQHSAGLDSL QVSGESLGGG RAAGSSSSVS ELETKASSDE ARSLPSTTQP
QQETTTKPTN RLRRLSSVDM NMKMEPQGKG KSEMPCVFTK GDGPNGKRVD GILYRYGSGE
EVRIMCVCHG DFLSPADFVK HAGGPHVDHP LRHIVVNTSS PSNLL
