ID   EG10_MDBVW              Reviewed;         233 AA.
AC   Q4ZJY8;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   23-FEB-2022, entry version 37.
DE   RecName: Full=Protease inhibitor Egf1.0;
DE   Flags: Precursor;
GN   Name=O12;
OS   Microplitis demolitor bracovirus (isolate Webb) (MdBV).
OC   Viruses; Polydnaviridae; Bracovirus.
OX   NCBI_TaxID=654919;
OH   NCBI_TaxID=69319; Microplitis demolitor.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=16380146; DOI=10.1016/j.virol.2005.11.010;
RA   Webb B.A., Strand M.R., Dickey S.E., Beck M.H., Hilgarth R.S., Barney W.E.,
RA   Kadash K., Kroemer J.A., Lindstrom K.G., Rattanadechakul W., Shelby K.S.,
RA   Thoetkiattikul H., Turnbull M.W., Witherell R.A.;
RT   "Polydnavirus genomes reflect their dual roles as mutualists and
RT   pathogens.";
RL   Virology 347:160-174(2006).
RN   [2]
RP   FUNCTION, AND MUTAGENESIS OF ARG-79.
RX   PubMed=18032603; DOI=10.1073/pnas.0708056104;
RA   Beck M.H., Strand M.R.;
RT   "A novel polydnavirus protein inhibits the insect prophenoloxidase
RT   activation pathway.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:19267-19272(2007).
RN   [3]
RP   INTERACTION WITH HOST PAP1 AND PAP3.
RX   PubMed=18519564; DOI=10.1074/jbc.m801593200;
RA   Lu Z., Beck M.H., Wang Y., Jiang H., Strand M.R.;
RT   "The viral protein Egf1.0 is a dual activity inhibitor of prophenoloxidase-
RT   activating proteinases 1 and 3 from Manduca sexta.";
RL   J. Biol. Chem. 283:21325-21333(2008).
CC   -!- FUNCTION: Counteracts the host humoral immune response by inhibiting
CC       the processing and the amidolytic activity of host PAP3. Thereby,
CC       melanization of host hemolymph, normally producing several reactive
CC       intermediates toxic for viruses, is deregulated and proper immune
CC       response cannot occur. {ECO:0000269|PubMed:18032603}.
CC   -!- SUBUNIT: Interacts with host PAP1 and PAP3.
CC       {ECO:0000269|PubMed:18519564}.
CC   -!- SIMILARITY: Belongs to the polydnaviridae EGF-like motif protein
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DQ000240; AAY24530.1; -; Genomic_DNA.
DR   RefSeq; YP_239421.1; NC_007044.1.
DR   GeneID; 3416073; -.
DR   KEGG; vg:3416073; -.
DR   Proteomes; UP000008168; Genome.
DR   GO; GO:0039503; P:suppression by virus of host innate immune response; IEA:UniProtKB-KW.
DR   InterPro; IPR036084; Ser_inhib-like_sf.
DR   SUPFAM; SSF57567; SSF57567; 1.
PE   1: Evidence at protein level;
KW   Host-virus interaction; Inhibition of host innate immune response by virus;
KW   Reference proteome; Signal; Viral immunoevasion.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..233
FT                   /note="Protease inhibitor Egf1.0"
FT                   /id="PRO_0000405388"
FT   DOMAIN          52..104
FT                   /note="TIL"
FT   REGION          201..233
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        218..233
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         79
FT                   /note="R->A: Loss of inhibitory activity."
FT                   /evidence="ECO:0000269|PubMed:18032603"
SQ   SEQUENCE   233 AA;  26734 MW;  26E338C0F8C8E3E7 CRC64;
     MYIDTGIMSN NIFLFAFFAL VGLTRIEAMP TKGSEGTWDV DYEDQEHTGI TCRENEHYNS
     TRIECEEECN DRNNKLCYRF QQFCWCNEGY IRNSSHICVK LEDCLKDEEQ KSETLASSAN
     NDSSKRLEDD LKLFSHDSVS HTSLEPETQA QKFNGIIDQE TLDLVFGKPE NSWAENKPLE
     TKTQAQKFNG KIDQETLDLV FGKPKNSSAE KKPLETETQA QKFNGIIDQE TLD