AFP2_ARATH
ID AFP2_ARATH Reviewed; 348 AA.
AC Q9LMX5; Q93ZQ8;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Ninja-family protein AFP2;
DE AltName: Full=ABI five-binding protein 2;
DE Short=ABI5-binding protein 2;
GN Name=AFP2; OrderedLocusNames=At1g13740; ORFNames=F21F23.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, NOMENCLATURE, FUNCTION, SUBUNIT, INTERACTION WITH ABI5/DPBF1;
RP DPBF2; AREB3/DPBF3; EEL/DPBF4; ABF1; ABF3/DPBF5 AND ABF4/AREB2, INDUCTION,
RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=18484180; DOI=10.1007/s11103-008-9344-2;
RA Garcia M.E., Lynch T.J., Peeters J., Snowden C., Finkelstein R.R.;
RT "A small plant-specific protein family of ABI five binding proteins (AFPs)
RT regulates stress response in germinating Arabidopsis seeds and seedlings.";
RL Plant Mol. Biol. 67:643-658(2008).
CC -!- FUNCTION: Acts as a negative regulator of abscisic acid (ABA) response
CC during germination through the ubiquitin-mediated proteolysis of
CC ABI5/DPBF1. {ECO:0000269|PubMed:18484180}.
CC -!- SUBUNIT: Forms a homodimer and heterodimer with AFP1 and AFP3.
CC Interacts with ABI5/DPBF1, DPBF2, AREB3/DPBF3, EEL/DPBF4, ABF1,
CC ABF3/DPBF5 and ABF4/AREB2. {ECO:0000269|PubMed:18484180}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18484180}.
CC -!- INDUCTION: Up-regulated by abscisic acid (ABA), glucose and salt.
CC {ECO:0000269|PubMed:18484180}.
CC -!- DISRUPTION PHENOTYPE: Exhibits hypersensitivity to salt, glucose and
CC osmotic stress and slight hypersensitivity to abscisic acid (ABA).
CC {ECO:0000269|PubMed:18484180}.
CC -!- SIMILARITY: Belongs to the Ninja family. {ECO:0000305}.
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DR EMBL; AC027656; AAF81300.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29062.1; -; Genomic_DNA.
DR EMBL; AY056326; AAL07175.1; -; mRNA.
DR EMBL; BT024887; ABD85158.1; -; mRNA.
DR PIR; G86270; G86270.
DR RefSeq; NP_563933.1; NM_101242.3.
DR AlphaFoldDB; Q9LMX5; -.
DR BioGRID; 23174; 13.
DR DIP; DIP-58589N; -.
DR IntAct; Q9LMX5; 11.
DR STRING; 3702.AT1G13740.1; -.
DR iPTMnet; Q9LMX5; -.
DR PaxDb; Q9LMX5; -.
DR PRIDE; Q9LMX5; -.
DR ProteomicsDB; 244682; -.
DR EnsemblPlants; AT1G13740.1; AT1G13740.1; AT1G13740.
DR GeneID; 837934; -.
DR Gramene; AT1G13740.1; AT1G13740.1; AT1G13740.
DR KEGG; ath:AT1G13740; -.
DR Araport; AT1G13740; -.
DR TAIR; locus:2023860; AT1G13740.
DR eggNOG; ENOG502QW6K; Eukaryota.
DR HOGENOM; CLU_034695_0_0_1; -.
DR InParanoid; Q9LMX5; -.
DR OMA; CNDERSP; -.
DR OrthoDB; 1080495at2759; -.
DR PhylomeDB; Q9LMX5; -.
DR PRO; PR:Q9LMX5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LMX5; baseline and differential.
DR Genevisible; Q9LMX5; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0009737; P:response to abscisic acid; IMP:TAIR.
DR GO; GO:0009414; P:response to water deprivation; IMP:TAIR.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR InterPro; IPR032310; NINJA_B.
DR InterPro; IPR031307; Ninja_fam.
DR InterPro; IPR012463; Ninja_motif.
DR InterPro; IPR032308; TDBD.
DR PANTHER; PTHR31413; PTHR31413; 1.
DR Pfam; PF07897; EAR; 1.
DR Pfam; PF16136; NINJA_B; 1.
DR Pfam; PF16135; TDBD; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..348
FT /note="Ninja-family protein AFP2"
FT /id="PRO_0000369615"
FT REGION 186..272
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 192..247
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 248..267
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 197
FT /note="S -> I (in Ref. 3; AAL07175)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 348 AA; 38211 MW; 0C7EEC22647A47D7 CRC64;
MGEASRQQRA WNREMTVTTN LSLDIDKYPR DLLRGFMSEN GGGRVFHGGE TNCDDESTIE
LNLGLSLGGR FGVDKTPRKL KRSSSVLDTV PFNDSTVAEP ENYTVGLERT TSLPAEMEEE
WRKRKEMQSL RRMEAKRRRC EKQSFRVGNS DDQTVSFENE RWVTASKSGF LQRHLVSSNR
QVCGVDSDGG GATGGGSSSS LSELDNKNQQ GSSNSCNDER SPKIVAGCSS NSGSQGTERP
SVTRANKVNE NENEKRVRSE DSVDRKGKGM ATSTGLVDMP CVFTKGDGPN GRRVDGILYK
YGKGEEVRIM CICHGSFLTP AEFVKHGGGG DVDRPLRHIV VNTSSSTF
