AFP3_ARATH
ID AFP3_ARATH Reviewed; 231 AA.
AC Q94F39; Q9LH16;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Ninja-family protein AFP3;
DE AltName: Full=ABI five-binding protein 3;
DE Short=ABI5-binding protein 3;
GN Name=AFP3; OrderedLocusNames=At3g29575; ORFNames=MWE13.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Nakamura Y.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. III.";
RL Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP GENE FAMILY, NOMENCLATURE, SUBUNIT, INTERACTION WITH ABI5/DPBF1; DPBF2;
RP AREB3/DPBF3; EEL/DPBF4; ABF1; ABF3/DPBF5 AND ABF4/AREB2, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=18484180; DOI=10.1007/s11103-008-9344-2;
RA Garcia M.E., Lynch T.J., Peeters J., Snowden C., Finkelstein R.R.;
RT "A small plant-specific protein family of ABI five binding proteins (AFPs)
RT regulates stress response in germinating Arabidopsis seeds and seedlings.";
RL Plant Mol. Biol. 67:643-658(2008).
CC -!- FUNCTION: Acts as a negative regulator of abscisic acid (ABA) response
CC and stress responses. {ECO:0000250}.
CC -!- SUBUNIT: Forms a heterodimer with AFP2. Interacts with ABI5/DPBF1,
CC DPBF2, AREB3/DPBF3, EEL/DPBF4, ABF1, ABF3/DPBF5 and ABF4/AREB2.
CC {ECO:0000269|PubMed:18484180}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- INDUCTION: Up-regulated by abscisic acid (ABA), glucose and salt.
CC {ECO:0000269|PubMed:18484180}.
CC -!- DISRUPTION PHENOTYPE: Exhibits hypersensitivity to salt and slight
CC hypersensitivity to glucose abscisic acid (ABA).
CC {ECO:0000269|PubMed:18484180}.
CC -!- SIMILARITY: Belongs to the Ninja family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01982.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP002457; BAB01982.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE77588.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77589.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77590.1; -; Genomic_DNA.
DR EMBL; AF385741; AAK60331.1; -; mRNA.
DR EMBL; AY133676; AAM91506.1; -; mRNA.
DR EMBL; AK317411; BAH20080.1; -; mRNA.
DR RefSeq; NP_001030794.1; NM_001035717.2.
DR RefSeq; NP_001078222.1; NM_001084753.2.
DR RefSeq; NP_189598.1; NM_113878.5.
DR AlphaFoldDB; Q94F39; -.
DR SMR; Q94F39; -.
DR BioGRID; 7947; 4.
DR DIP; DIP-40552N; -.
DR IntAct; Q94F39; 12.
DR STRING; 3702.AT3G29575.1; -.
DR iPTMnet; Q94F39; -.
DR PaxDb; Q94F39; -.
DR PRIDE; Q94F39; -.
DR ProteomicsDB; 244767; -.
DR EnsemblPlants; AT3G29575.1; AT3G29575.1; AT3G29575.
DR EnsemblPlants; AT3G29575.3; AT3G29575.3; AT3G29575.
DR EnsemblPlants; AT3G29575.4; AT3G29575.4; AT3G29575.
DR GeneID; 822619; -.
DR Gramene; AT3G29575.1; AT3G29575.1; AT3G29575.
DR Gramene; AT3G29575.3; AT3G29575.3; AT3G29575.
DR Gramene; AT3G29575.4; AT3G29575.4; AT3G29575.
DR KEGG; ath:AT3G29575; -.
DR Araport; AT3G29575; -.
DR TAIR; locus:2094167; AT3G29575.
DR eggNOG; ENOG502QW6K; Eukaryota.
DR HOGENOM; CLU_034695_0_0_1; -.
DR InParanoid; Q94F39; -.
DR OMA; NPAPLMG; -.
DR OrthoDB; 1469925at2759; -.
DR PhylomeDB; Q94F39; -.
DR PRO; PR:Q94F39; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q94F39; baseline and differential.
DR Genevisible; Q94F39; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0009737; P:response to abscisic acid; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR InterPro; IPR032310; NINJA_B.
DR InterPro; IPR031307; Ninja_fam.
DR InterPro; IPR012463; Ninja_motif.
DR InterPro; IPR032308; TDBD.
DR PANTHER; PTHR31413; PTHR31413; 1.
DR Pfam; PF07897; EAR; 1.
DR Pfam; PF16136; NINJA_B; 1.
DR Pfam; PF16135; TDBD; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..231
FT /note="Ninja-family protein AFP3"
FT /id="PRO_0000369616"
FT REGION 83..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 109..152
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 231 AA; 25243 MW; 7CE5267AC501012D CRC64;
MSKKQRLSEE DGEVEIELDL GLSLNGRFGV DPLAKTRLMR STSVLDLVVN DRSGLSRTCS
LPVETEEEWR KRKELQSLRR LEAKRKRSEK QRKHKACGGE EKVVEEGSIG SSGSGSSGLS
EVDTLLPPVQ ATTNKSVETS PSSAQSQPEN LGKEASQNII EDMPFVSTTG DGPNGKKING
FLYRYRKGEE VRIVCVCHGS FLSPAEFVKH AGGGDVAHPL KHIVVNPSPF L
