EGFL8_HUMAN
ID EGFL8_HUMAN Reviewed; 293 AA.
AC Q99944; B0S884; G5E9Q0; Q5JP23; Q5SSX3; Q8IV30;
DT 25-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Epidermal growth factor-like protein 8;
DE Short=EGF-like protein 8;
DE AltName: Full=Vascular endothelial statin-2;
DE Short=VE-statin-2;
DE Flags: Precursor;
GN Name=EGFL8; Synonyms=C6orf8, NG3; ORFNames=UNQ8752/PRO29920;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Tanaka S.;
RT "A novel gene regulating tumor angiogenesis.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14656967; DOI=10.1101/gr.1736803;
RA Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D.,
RA Hood L.;
RT "Analysis of the gene-dense major histocompatibility complex class III
RT region and its comparison to mouse.";
RL Genome Res. 13:2621-2636(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT LYS-86.
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PROTEIN SEQUENCE OF 26-40.
RX PubMed=15340161; DOI=10.1110/ps.04682504;
RA Zhang Z., Henzel W.J.;
RT "Signal peptide prediction based on analysis of experimentally verified
RT cleavage sites.";
RL Protein Sci. 13:2819-2824(2004).
CC -!- INTERACTION:
CC Q99944; Q86V38: ATN1; NbExp=3; IntAct=EBI-3924130, EBI-11954292;
CC Q99944; P27797: CALR; NbExp=3; IntAct=EBI-3924130, EBI-1049597;
CC Q99944; P02489: CRYAA; NbExp=4; IntAct=EBI-3924130, EBI-6875961;
CC Q99944; P36957: DLST; NbExp=3; IntAct=EBI-3924130, EBI-351007;
CC Q99944; P00488: F13A1; NbExp=3; IntAct=EBI-3924130, EBI-2565863;
CC Q99944; P10809: HSPD1; NbExp=3; IntAct=EBI-3924130, EBI-352528;
CC Q99944; Q8WXH2: JPH3; NbExp=3; IntAct=EBI-3924130, EBI-1055254;
CC Q99944; Q8TDX7: NEK7; NbExp=3; IntAct=EBI-3924130, EBI-1055945;
CC Q99944; P16284: PECAM1; NbExp=3; IntAct=EBI-3924130, EBI-716404;
CC Q99944; P04275-2: VWF; NbExp=3; IntAct=EBI-3924130, EBI-25896548;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
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DR EMBL; AB190520; BAD51398.1; -; mRNA.
DR EMBL; AY358139; AAQ88506.1; -; mRNA.
DR EMBL; U89336; AAB47494.1; -; Genomic_DNA.
DR EMBL; AL662828; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL662884; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL845464; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX284686; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX927239; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CR933878; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CR812478; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471081; EAX03598.1; -; Genomic_DNA.
DR EMBL; BC035574; AAH35574.1; -; mRNA.
DR EMBL; BC052591; AAH52591.1; -; mRNA.
DR CCDS; CCDS4743.1; -.
DR RefSeq; NP_085155.1; NM_030652.3.
DR AlphaFoldDB; Q99944; -.
DR SMR; Q99944; -.
DR BioGRID; 123336; 57.
DR IntAct; Q99944; 18.
DR STRING; 9606.ENSP00000378888; -.
DR GlyGen; Q99944; 1 site.
DR iPTMnet; Q99944; -.
DR PhosphoSitePlus; Q99944; -.
DR BioMuta; EGFL8; -.
DR DMDM; 55584040; -.
DR jPOST; Q99944; -.
DR MassIVE; Q99944; -.
DR PaxDb; Q99944; -.
DR PeptideAtlas; Q99944; -.
DR PRIDE; Q99944; -.
DR ProteomicsDB; 34007; -.
DR ProteomicsDB; 78535; -.
DR Antibodypedia; 34956; 69 antibodies from 21 providers.
DR DNASU; 80864; -.
DR Ensembl; ENST00000333845.11; ENSP00000333380.6; ENSG00000241404.7.
DR Ensembl; ENST00000395512.5; ENSP00000378888.1; ENSG00000241404.7.
DR Ensembl; ENST00000399648.5; ENSP00000382556.1; ENSG00000242038.5.
DR Ensembl; ENST00000413733.5; ENSP00000390866.1; ENSG00000239974.6.
DR Ensembl; ENST00000414111.6; ENSP00000415577.2; ENSG00000240389.5.
DR Ensembl; ENST00000418252.5; ENSP00000414996.1; ENSG00000244444.6.
DR Ensembl; ENST00000433364.5; ENSP00000388000.1; ENSG00000240389.5.
DR Ensembl; ENST00000436155.5; ENSP00000405502.1; ENSG00000243897.8.
DR Ensembl; ENST00000443511.6; ENSP00000394193.2; ENSG00000244444.6.
DR Ensembl; ENST00000447331.6; ENSP00000407236.2; ENSG00000242038.5.
DR Ensembl; ENST00000450526.5; ENSP00000398893.1; ENSG00000243897.8.
DR Ensembl; ENST00000452189.6; ENSP00000397426.2; ENSG00000239974.6.
DR Ensembl; ENST00000452290.5; ENSP00000416366.1; ENSG00000240592.5.
DR Ensembl; ENST00000455889.6; ENSP00000411023.2; ENSG00000240592.5.
DR GeneID; 80864; -.
DR KEGG; hsa:80864; -.
DR MANE-Select; ENST00000333845.11; ENSP00000333380.6; NM_030652.4; NP_085155.1.
DR UCSC; uc003oab.2; human.
DR CTD; 80864; -.
DR DisGeNET; 80864; -.
DR GeneCards; EGFL8; -.
DR HGNC; HGNC:13944; EGFL8.
DR HPA; ENSG00000241404; Low tissue specificity.
DR MIM; 609897; gene.
DR neXtProt; NX_Q99944; -.
DR OpenTargets; ENSG00000241404; -.
DR PharmGKB; PA38372; -.
DR VEuPathDB; HostDB:ENSG00000241404; -.
DR eggNOG; KOG1217; Eukaryota.
DR GeneTree; ENSGT00940000162975; -.
DR HOGENOM; CLU_083642_0_0_1; -.
DR InParanoid; Q99944; -.
DR OMA; LGTCSCE; -.
DR OrthoDB; 749722at2759; -.
DR TreeFam; TF331360; -.
DR PathwayCommons; Q99944; -.
DR SignaLink; Q99944; -.
DR BioGRID-ORCS; 80864; 13 hits in 1068 CRISPR screens.
DR GenomeRNAi; 80864; -.
DR Pharos; Q99944; Tbio.
DR PRO; PR:Q99944; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q99944; protein.
DR Bgee; ENSG00000241404; Expressed in tibial nerve and 90 other tissues.
DR ExpressionAtlas; Q99944; baseline and differential.
DR Genevisible; Q99944; HS.
DR GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005102; F:signaling receptor binding; IBA:GO_Central.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR InterPro; IPR001881; EGF-like_Ca-bd_dom.
DR InterPro; IPR000742; EGF-like_dom.
DR InterPro; IPR000152; EGF-type_Asp/Asn_hydroxyl_site.
DR InterPro; IPR018097; EGF_Ca-bd_CS.
DR InterPro; IPR011489; EMI_domain.
DR Pfam; PF00008; EGF; 1.
DR Pfam; PF07645; EGF_CA; 1.
DR Pfam; PF07546; EMI; 1.
DR SMART; SM00181; EGF; 2.
DR SMART; SM00179; EGF_CA; 1.
DR PROSITE; PS00010; ASX_HYDROXYL; 1.
DR PROSITE; PS00022; EGF_1; 1.
DR PROSITE; PS01186; EGF_2; 1.
DR PROSITE; PS50026; EGF_3; 2.
DR PROSITE; PS01187; EGF_CA; 1.
DR PROSITE; PS51041; EMI; 1.
PE 1: Evidence at protein level;
KW Calcium; Coiled coil; Direct protein sequencing; Disulfide bond;
KW EGF-like domain; Glycoprotein; Reference proteome; Repeat; Secreted;
KW Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000269|PubMed:15340161"
FT CHAIN 26..293
FT /note="Epidermal growth factor-like protein 8"
FT /id="PRO_0000007531"
FT DOMAIN 34..112
FT /note="EMI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00384"
FT DOMAIN 111..142
FT /note="EGF-like 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DOMAIN 144..184
FT /note="EGF-like 2; calcium-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT COILED 195..232
FT /evidence="ECO:0000255"
FT CARBOHYD 50
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 38..97
FT /evidence="ECO:0000250"
FT DISULFID 65..71
FT /evidence="ECO:0000250"
FT DISULFID 96..110
FT /evidence="ECO:0000250"
FT DISULFID 114..124
FT /evidence="ECO:0000250"
FT DISULFID 118..130
FT /evidence="ECO:0000250"
FT DISULFID 132..141
FT /evidence="ECO:0000250"
FT DISULFID 148..159
FT /evidence="ECO:0000250"
FT DISULFID 155..168
FT /evidence="ECO:0000250"
FT DISULFID 170..183
FT /evidence="ECO:0000250"
FT VARIANT 86
FT /note="R -> K (in dbSNP:rs3096697)"
FT /evidence="ECO:0000269|PubMed:14574404"
FT /id="VAR_019792"
FT VARIANT 204
FT /note="A -> E (in dbSNP:rs2071289)"
FT /id="VAR_019793"
FT VARIANT 277
FT /note="G -> C (in dbSNP:rs35587174)"
FT /id="VAR_048983"
FT CONFLICT 273
FT /note="E -> Q (in Ref. 6; AAH35574)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 293 AA; 32262 MW; 6519CA254FB23FD0 CRC64;
MGSRAELCTL LGGFSFLLLL IPGEGAKGGS LRESQGVCSK QTLVVPLHYN ESYSQPVYKP
YLTLCAGRRI CSTYRTMYRV MWREVRREVQ QTHAVCCQGW KKRHPGALTC EAICAKPCLN
GGVCVRPDQC ECAPGWGGKH CHVDVDECRT SITLCSHHCF NTAGSFTCGC PHDLVLGVDG
RTCMEGSPEP PTSASILSVA VREAEKDERA LKQEIHELRG RLERLEQWAG QAGAWVRAVL
PVPPEELQPE QVAELWGRGD RIESLSDQVL LLEERLGACS CEDNSLGLGV NHR
