EGFL8_MOUSE
ID EGFL8_MOUSE Reviewed; 293 AA.
AC Q6GUQ1; O35447; Q3UFB4;
DT 25-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Epidermal growth factor-like protein 8;
DE Short=EGF-like protein 8;
DE Flags: Precursor;
GN Name=Egfl8; Synonyms=Ng3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=CD-1;
RX PubMed=15162510; DOI=10.1002/dvdy.20063;
RA Fitch M.J., Campagnolo L., Kuhnert F., Stuhlmann H.;
RT "Egfl7, a novel epidermal growth factor-domain gene expressed in
RT endothelial cells.";
RL Dev. Dyn. 230:316-324(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Sympathetic ganglion;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=129;
RX PubMed=14656967; DOI=10.1101/gr.1736803;
RA Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D.,
RA Hood L.;
RT "Analysis of the gene-dense major histocompatibility complex class III
RT region and its comparison to mouse.";
RL Genome Res. 13:2621-2636(2003).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6GUQ1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6GUQ1-2; Sequence=VSP_026672;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed in brain, kidney, thymus and
CC lung. {ECO:0000269|PubMed:15162510}.
CC -!- DEVELOPMENTAL STAGE: Not detected before 11.5 dpc and expression levels
CC vary between 11.5 dpc and 15.5 dpc. {ECO:0000269|PubMed:15162510}.
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DR EMBL; AY635583; AAT47548.1; -; mRNA.
DR EMBL; AK148723; BAE28647.1; -; mRNA.
DR EMBL; AF030001; AAB82010.1; -; Genomic_DNA.
DR CCDS; CCDS28651.1; -. [Q6GUQ1-1]
DR PIR; T09065; T09065.
DR RefSeq; NP_690886.3; NM_152922.3. [Q6GUQ1-1]
DR RefSeq; XP_006525242.1; XM_006525179.3. [Q6GUQ1-1]
DR AlphaFoldDB; Q6GUQ1; -.
DR STRING; 10090.ENSMUSP00000015611; -.
DR GlyGen; Q6GUQ1; 1 site.
DR iPTMnet; Q6GUQ1; -.
DR PhosphoSitePlus; Q6GUQ1; -.
DR PaxDb; Q6GUQ1; -.
DR PRIDE; Q6GUQ1; -.
DR ProteomicsDB; 277836; -. [Q6GUQ1-1]
DR ProteomicsDB; 277837; -. [Q6GUQ1-2]
DR Antibodypedia; 34956; 69 antibodies from 21 providers.
DR DNASU; 81701; -.
DR Ensembl; ENSMUST00000015611; ENSMUSP00000015611; ENSMUSG00000015467. [Q6GUQ1-1]
DR Ensembl; ENSMUST00000097345; ENSMUSP00000094958; ENSMUSG00000015467. [Q6GUQ1-2]
DR GeneID; 81701; -.
DR KEGG; mmu:81701; -.
DR UCSC; uc008cdb.2; mouse. [Q6GUQ1-1]
DR UCSC; uc008cdc.2; mouse. [Q6GUQ1-2]
DR CTD; 80864; -.
DR MGI; MGI:1932094; Egfl8.
DR VEuPathDB; HostDB:ENSMUSG00000015467; -.
DR eggNOG; KOG1217; Eukaryota.
DR GeneTree; ENSGT00940000162975; -.
DR InParanoid; Q6GUQ1; -.
DR OMA; LGTCSCE; -.
DR PhylomeDB; Q6GUQ1; -.
DR TreeFam; TF331360; -.
DR BioGRID-ORCS; 81701; 1 hit in 72 CRISPR screens.
DR PRO; PR:Q6GUQ1; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q6GUQ1; protein.
DR Bgee; ENSMUSG00000015467; Expressed in thymus and 60 other tissues.
DR ExpressionAtlas; Q6GUQ1; baseline and differential.
DR Genevisible; Q6GUQ1; MM.
DR GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; NAS:UniProtKB.
DR GO; GO:0005102; F:signaling receptor binding; IBA:GO_Central.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR InterPro; IPR001881; EGF-like_Ca-bd_dom.
DR InterPro; IPR000742; EGF-like_dom.
DR InterPro; IPR000152; EGF-type_Asp/Asn_hydroxyl_site.
DR InterPro; IPR018097; EGF_Ca-bd_CS.
DR InterPro; IPR011489; EMI_domain.
DR InterPro; IPR009030; Growth_fac_rcpt_cys_sf.
DR Pfam; PF07645; EGF_CA; 1.
DR Pfam; PF07546; EMI; 1.
DR SMART; SM00181; EGF; 2.
DR SMART; SM00179; EGF_CA; 1.
DR SUPFAM; SSF57184; SSF57184; 1.
DR PROSITE; PS00010; ASX_HYDROXYL; 1.
DR PROSITE; PS00022; EGF_1; 1.
DR PROSITE; PS01186; EGF_2; 1.
DR PROSITE; PS50026; EGF_3; 2.
DR PROSITE; PS01187; EGF_CA; 1.
DR PROSITE; PS51041; EMI; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calcium; Coiled coil; Disulfide bond;
KW EGF-like domain; Glycoprotein; Reference proteome; Repeat; Secreted;
KW Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..293
FT /note="Epidermal growth factor-like protein 8"
FT /id="PRO_0000007532"
FT DOMAIN 34..112
FT /note="EMI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00384"
FT DOMAIN 111..142
FT /note="EGF-like 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DOMAIN 144..184
FT /note="EGF-like 2; calcium-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT COILED 206..235
FT /evidence="ECO:0000255"
FT CARBOHYD 50
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 38..97
FT /evidence="ECO:0000250"
FT DISULFID 65..71
FT /evidence="ECO:0000250"
FT DISULFID 96..110
FT /evidence="ECO:0000250"
FT DISULFID 114..124
FT /evidence="ECO:0000250"
FT DISULFID 118..130
FT /evidence="ECO:0000250"
FT DISULFID 132..141
FT /evidence="ECO:0000250"
FT DISULFID 148..159
FT /evidence="ECO:0000250"
FT DISULFID 155..168
FT /evidence="ECO:0000250"
FT DISULFID 170..183
FT /evidence="ECO:0000250"
FT VAR_SEQ 240..269
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_026672"
FT CONFLICT 26
FT /note="T -> A (in Ref. 1; AAT47548)"
FT /evidence="ECO:0000305"
FT CONFLICT 231
FT /note="T -> K (in Ref. 1; AAT47548)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 293 AA; 32066 MW; 8A99A5EC000A2C62 CRC64;
MGLWAELCIS LRGLSFFLVL MTGEGTRGGS FKESLGVCSK QTLLVPLRYN ESYSQPVYKP
YLTLCAGRRI CSTYRTTYRV AWREVRREVP QTHVVCCQGW KKPHPGALTC DAICSKPCLN
GGVCTGPDRC ECAPGWGGKH CHVDVDECRA SLTLCSHGCL NTLGSFLCSC PHPLVLGLDG
RTCAGGPPES PTSASILSVA VREADSEEER ALRWEVAELR GRLEKLEQWA TQAGAWVRAV
LPMPPEELRP EQVAELWGRG DRIESLSDQV LLLEERLGAC ACEDNSLGPS LRG
