AFP4_ARATH
ID AFP4_ARATH Reviewed; 319 AA.
AC Q9S7Z2;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Ninja-family protein AFP4;
DE AltName: Full=ABI five-binding protein 4;
DE Short=ABI5-binding protein 4;
DE AltName: Full=Protein TWO OR MORE ABRES-CONTAINING GENE 2;
GN Name=AFP4; Synonyms=TMAC2; OrderedLocusNames=At3g02140;
GN ORFNames=F14P3.21, F1C9.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=17195036; DOI=10.1007/s11103-006-9109-8;
RA Huang M.-D., Wu W.-L.;
RT "Overexpression of TMAC2, a novel negative regulator of abscisic acid and
RT salinity responses, has pleiotropic effects in Arabidopsis thaliana.";
RL Plant Mol. Biol. 63:557-569(2007).
RN [5]
RP GENE FAMILY, NOMENCLATURE, SUBUNIT, INTERACTION WITH ABI5/DPBF1;
RP AREB3/DPBF3; EEL/DPBF4; ABF1 AND ABF3/DPBF5, INDUCTION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=18484180; DOI=10.1007/s11103-008-9344-2;
RA Garcia M.E., Lynch T.J., Peeters J., Snowden C., Finkelstein R.R.;
RT "A small plant-specific protein family of ABI five binding proteins (AFPs)
RT regulates stress response in germinating Arabidopsis seeds and seedlings.";
RL Plant Mol. Biol. 67:643-658(2008).
CC -!- FUNCTION: Acts as a negative regulator of abscisic acid (ABA) and
CC salinity responses. {ECO:0000269|PubMed:17195036}.
CC -!- SUBUNIT: Interacts with ABI5/DPBF1, AREB3/DPBF3, EEL/DPBF4, ABF1 and
CC ABF3/DPBF5. {ECO:0000269|PubMed:18484180}.
CC -!- INTERACTION:
CC Q9S7Z2; Q9ZW21: At2g29380; NbExp=4; IntAct=EBI-1778843, EBI-4441103;
CC Q9S7Z2; Q17TI5: BRX; NbExp=3; IntAct=EBI-1778843, EBI-4426649;
CC Q9S7Z2; O23160: MYB73; NbExp=3; IntAct=EBI-1778843, EBI-25506855;
CC Q9S7Z2; Q38845: PP2AA1; NbExp=3; IntAct=EBI-1778843, EBI-1645478;
CC Q9S7Z2; Q8GY55: TIFY4B; NbExp=3; IntAct=EBI-1778843, EBI-15206004;
CC Q9S7Z2; Q5CCK4: VAL2; NbExp=3; IntAct=EBI-1778843, EBI-15193683;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17195036}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in roots and seedlings.
CC {ECO:0000269|PubMed:17195036}.
CC -!- INDUCTION: Up-regulated by abscisic acid (ABA) and salt. Down-regulated
CC by cold and glucose. {ECO:0000269|PubMed:17195036,
CC ECO:0000269|PubMed:18484180}.
CC -!- DISRUPTION PHENOTYPE: Exhibits insensitivity to abscisic acid (ABA) and
CC salt. {ECO:0000269|PubMed:18484180}.
CC -!- SIMILARITY: Belongs to the Ninja family. {ECO:0000305}.
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DR EMBL; AC009755; AAF02123.1; -; Genomic_DNA.
DR EMBL; AC011664; AAF14824.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE73768.1; -; Genomic_DNA.
DR EMBL; AY034949; AAK59455.1; -; mRNA.
DR EMBL; AY062992; AAL34166.1; -; mRNA.
DR RefSeq; NP_566163.1; NM_111081.3.
DR AlphaFoldDB; Q9S7Z2; -.
DR BioGRID; 6515; 14.
DR IntAct; Q9S7Z2; 15.
DR STRING; 3702.AT3G02140.1; -.
DR iPTMnet; Q9S7Z2; -.
DR PaxDb; Q9S7Z2; -.
DR PRIDE; Q9S7Z2; -.
DR ProteomicsDB; 244768; -.
DR EnsemblPlants; AT3G02140.1; AT3G02140.1; AT3G02140.
DR GeneID; 821182; -.
DR Gramene; AT3G02140.1; AT3G02140.1; AT3G02140.
DR KEGG; ath:AT3G02140; -.
DR Araport; AT3G02140; -.
DR TAIR; locus:2076502; AT3G02140.
DR eggNOG; ENOG502QW6K; Eukaryota.
DR HOGENOM; CLU_034695_0_0_1; -.
DR InParanoid; Q9S7Z2; -.
DR OMA; IASNHFC; -.
DR OrthoDB; 1469925at2759; -.
DR PhylomeDB; Q9S7Z2; -.
DR PRO; PR:Q9S7Z2; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9S7Z2; baseline and differential.
DR Genevisible; Q9S7Z2; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0009788; P:negative regulation of abscisic acid-activated signaling pathway; IMP:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0010581; P:regulation of starch biosynthetic process; IMP:TAIR.
DR GO; GO:0009737; P:response to abscisic acid; IEP:TAIR.
DR GO; GO:0009651; P:response to salt stress; IEP:TAIR.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR InterPro; IPR031307; Ninja_fam.
DR InterPro; IPR012463; Ninja_motif.
DR InterPro; IPR032308; TDBD.
DR PANTHER; PTHR31413; PTHR31413; 1.
DR Pfam; PF07897; EAR; 1.
DR Pfam; PF16135; TDBD; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..319
FT /note="Ninja-family protein AFP4"
FT /id="PRO_0000369617"
FT REGION 39..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 99..120
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 205..228
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 39..57
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 211..228
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 319 AA; 35455 MW; 275D25D6926FF5A0 CRC64;
MEMIRGRGIP NGVVQVPNLS ANNLLQKFFI ASNHFCQRDS EHGENQQEAK KREDEAEEDE
KDVELTLGLS LNGQFGTDPR SRKRRHFELG RSSSIPEGFV FDEQRSGGGN GGDMRRIVGR
GGSDMFQLDR TRSLPVVTEM DIEKERKVSE KTRAFMESPV TNRGAYLTKD KNRGQAVETE
KPRAFLEFKI PPTKEGKKEK DRLVVTGPVN GKGKNGNTAK KQKNNVENSG MEKARNILED
MPCVSTRDVG ADGKRVEGFL YWYGGNKEEV KIVCVCHGSF LSPAEFVRHG GGTVSDDDGG
DVMINPLRHI VVKLPSSSI
