ID   EGF_LYMST               Reviewed;          64 AA.
AC   Q9GU69;
DT   29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=Epidermal growth factor;
DE            Short=L-EGF;
DE   Flags: Precursor;
OS   Lymnaea stagnalis (Great pond snail) (Helix stagnalis).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Heterobranchia; Euthyneura; Panpulmonata; Hygrophila; Lymnaeoidea;
OC   Lymnaeidae; Lymnaea.
OX   NCBI_TaxID=6523;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-64, PYROGLUTAMATE
RP   FORMATION AT GLN-22, MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION,
RP   TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC   TISSUE=Albumen gland;
RX   PubMed=10964941; DOI=10.1523/jneurosci.20-17-06355.2000;
RA   Hermann P.M., van Kesteren R.E., Wildering W.C., Painter S.D., Reno J.M.,
RA   Smith J.S., Kumar S.B., Geraerts W.P., Ericsson L.H., Smit A.B.,
RA   Bulloch A.G., Nagle G.T.;
RT   "Neurotrophic actions of a novel molluscan epidermal growth factor.";
RL   J. Neurosci. 20:6355-6364(2000).
CC   -!- FUNCTION: Induces neurite outgrowth in specific adult neurons in vitro
CC       (PubMed:10964941). {ECO:0000269|PubMed:10964941}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000303|PubMed:10964941}.
CC   -!- TISSUE SPECIFICITY: Albumen gland. Up-regulated in adult CNS after
CC       axotomy. {ECO:0000269|PubMed:10964941}.
CC   -!- DEVELOPMENTAL STAGE: Expressed throughout embryonic development, in the
CC       juvenile CNS, but not in the normal adult.
CC       {ECO:0000269|PubMed:10964941}.
CC   -!- MASS SPECTROMETRY: Mass=4541; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:10964941};
CC   -!- MASS SPECTROMETRY: Mass=4547; Method=MALDI; Note=Reduced with 2-
CC       mercaptoethanol.; Evidence={ECO:0000269|PubMed:10964941};
CC   -!- MASS SPECTROMETRY: Mass=5177; Method=MALDI; Note=Reduced with 2-
CC       mercaptoethanol and alkylated with 4-vinylpyridine.;
CC       Evidence={ECO:0000269|PubMed:10964941};
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DR   EMBL; AF187041; AAG17020.1; -; mRNA.
DR   PIR; A58998; A58998.
DR   AlphaFoldDB; Q9GU69; -.
DR   SMR; Q9GU69; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR000742; EGF-like_dom.
DR   Pfam; PF00008; EGF; 1.
DR   SMART; SM00181; EGF; 1.
DR   PROSITE; PS00022; EGF_1; 1.
DR   PROSITE; PS01186; EGF_2; 1.
DR   PROSITE; PS50026; EGF_3; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; EGF-like domain; Growth factor;
KW   Pyrrolidone carboxylic acid; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:10964941"
FT   CHAIN           22..64
FT                   /note="Epidermal growth factor"
FT                   /id="PRO_0000432755"
FT   DOMAIN          25..61
FT                   /note="EGF-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   MOD_RES         22
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:10964941"
FT   DISULFID        29..40
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        34..49
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        51..60
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
SQ   SEQUENCE   64 AA;  6816 MW;  46877952E77452FC CRC64;
     MMRHLLLVGA AILIFVSDAQ AQGDGEDPCQ IVRCSYGANC IAYGDTAICE CPFGYSGIRC
     QDPS