ID   AFP4_MYOOC              Reviewed;         128 AA.
AC   P80961; O73699;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   18-OCT-2001, sequence version 2.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Type-4 ice-structuring protein LS-12;
DE            Short=ISP LS-12;
DE   AltName: Full=Antifreeze protein LS-12;
DE   Flags: Precursor;
OS   Myoxocephalus octodecemspinosus (Longhorn sculpin) (Cottus
OS   octodecemspinosus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Perciformes; Cottioidei; Cottales; Cottidae; Myoxocephalus.
OX   NCBI_TaxID=68557;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   PubMed=9540788; DOI=10.1016/s0167-4838(97)00197-0;
RA   Zhao Z., Deng G., Lui Q., Laursen R.A.;
RT   "Cloning and sequencing of cDNA encoding the LS-12 antifreeze protein in
RT   the longhorn sculpin, Myoxocephalus octodecimspinosis.";
RL   Biochim. Biophys. Acta 1382:177-180(1998).
RN   [2]
RP   PROTEIN SEQUENCE OF 21-128, AND PYROGLUTAMATE FORMATION AT GLN-21.
RC   TISSUE=Blood;
RX   PubMed=9013849; DOI=10.1016/s0014-5793(96)01466-4;
RA   Deng G., Andrews D.W., Laursen R.A.;
RT   "Amino acid sequence of a new type of antifreeze protein, from the longhorn
RT   sculpin Myoxocephalus octodecimspinosis.";
RL   FEBS Lett. 402:17-20(1997).
CC   -!- FUNCTION: Antifreeze proteins lower the blood freezing point.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the apolipoprotein A1/A4/E family.
CC       {ECO:0000305}.
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DR   EMBL; AF026525; AAC27986.1; -; mRNA.
DR   PIR; A59010; A59010.
DR   AlphaFoldDB; P80961; -.
DR   SMR; P80961; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Antifreeze protein; Direct protein sequencing; Pyrrolidone carboxylic acid;
KW   Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000269|PubMed:9013849"
FT   CHAIN           21..128
FT                   /note="Type-4 ice-structuring protein LS-12"
FT                   /id="PRO_0000001698"
FT   MOD_RES         21
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:9013849"
SQ   SEQUENCE   128 AA;  14378 MW;  FC9688BD6E32F55D CRC64;
     MKFSLVATIV LLALAQGSFA QGAADLESLG QYFEEMKTKL IQDMTEIIRS QDLANQAQAF
     VEDKKTQLQP LVAQIQEQMK TVATNVEEQI RPLTANVQAH LQPQIDNFQK QMEAIIKKLT
     DQTMAIEN