EGL13_CAEEL
ID EGL13_CAEEL Reviewed; 470 AA.
AC Q23045; H2L0M3; Q4PIT4; Q86G49; Q9TZN5;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Transcription factor egl-13 {ECO:0000305|PubMed:14668410};
DE AltName: Full=Protein egg laying defective 13 {ECO:0000312|WormBase:T22B7.1a};
GN Name=egl-13 {ECO:0000312|WormBase:T22B7.1a};
GN Synonyms=cog-2 {ECO:0000312|WormBase:T22B7.1a};
GN ORFNames=T22B7.1 {ECO:0000312|WormBase:T22B7.1a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000312|EMBL:AAD12252.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND
RP MUTAGENESIS OF ALA-335.
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:AAD12252.1};
RX PubMed=9834196; DOI=10.1242/dev.126.1.169;
RA Hanna-Rose W., Han M.;
RT "COG-2, a sox domain protein necessary for establishing a functional
RT vulval-uterine connection in Caenorhabditis elegans.";
RL Development 126:169-179(1999).
RN [2] {ECO:0000312|EMBL:CCD74407.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF ARG-331 AND PRO-394.
RX PubMed=14668410; DOI=10.1093/genetics/165.3.1623;
RA Cinar H.N., Richards K.L., Oommen K.S., Newman A.P.;
RT "The EGL-13 SOX domain transcription factor affects the uterine pi cell
RT lineages in Caenorhabditis elegans.";
RL Genetics 165:1623-1628(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF
RP 177-GLN--GLU-486.
RX PubMed=23946438; DOI=10.1242/dev.098723;
RA Feng G., Yi P., Yang Y., Chai Y., Tian D., Zhu Z., Liu J., Zhou F.,
RA Cheng Z., Wang X., Li W., Ou G.;
RT "Developmental stage-dependent transcriptional regulatory pathways control
RT neuroblast lineage progression.";
RL Development 140:3838-3847(2013).
RN [5] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE,
RP AND MUTAGENESIS OF GLY-364.
RX PubMed=23671427; DOI=10.1371/journal.pgen.1003511;
RA Gramstrup Petersen J., Rojo Romanos T., Juozaityte V., Redo Riveiro A.,
RA Hums I., Traunmuller L., Zimmer M., Pocock R.;
RT "EGL-13/SoxD specifies distinct O2 and CO2 sensory neuron fates in
RT Caenorhabditis elegans.";
RL PLoS Genet. 9:E1003511-E1003511(2013).
CC -!- FUNCTION: Probable transcription factor that is required for uterine
CC and neuronal cell fate decisions (PubMed:9834196, PubMed:14668410,
CC PubMed:23946438). Controls genes required for the specification and
CC differentiation of O(2) and CO(2)-sensing neurons and for maintaining
CC URX sensory neuronal cell fate (PubMed:23671427).
CC {ECO:0000269|PubMed:14668410, ECO:0000269|PubMed:23671427,
CC ECO:0000269|PubMed:9834196}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267,
CC ECO:0000269|PubMed:23946438, ECO:0000269|PubMed:9834196}.
CC Note=Localized to nucleus during interphase.
CC {ECO:0000269|PubMed:23946438}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=a {ECO:0000312|WormBase:T22B7.1a};
CC IsoId=Q23045-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:T22B7.1b};
CC IsoId=Q23045-2; Sequence=VSP_057621;
CC Name=c {ECO:0000312|WormBase:T22B7.1c};
CC IsoId=Q23045-3; Sequence=VSP_057622;
CC Name=d {ECO:0000312|WormBase:T22B7.1d};
CC IsoId=Q23045-4; Sequence=VSP_057620;
CC -!- TISSUE SPECIFICITY: Expressed in body wall muscle and head and tail
CC neurons (PubMed:9834196). Specifically expressed in O(2) and CO(2)-
CC sensing neurons, including BAG and URX sensory neurons
CC (PubMed:23671427). {ECO:0000269|PubMed:23671427,
CC ECO:0000269|PubMed:9834196}.
CC -!- DEVELOPMENTAL STAGE: Expressed following fertilization and during
CC embryogenesis in four neuronal cells (PubMed:23671427). Expressed in
CC BAG, URX, AQR and PQR sensory neurons and cells in the head and tail in
CC L1 larval stage and in the body wall muscle and vulval cells later in
CC larval development (PubMed:23671427, PubMed:23946438). Expressed in
CC cells of the pi uterine cell lineage from late L3 to mid L4
CC (PubMed:9834196). {ECO:0000269|PubMed:23671427,
CC ECO:0000269|PubMed:9834196}.
CC -!- DISRUPTION PHENOTYPE: Hermaphrodites have an egg laying defective
CC phenotype and a reduced brood size (PubMed:9834196). They display a
CC protruding vulva phenotype and there is a failure of anchor cells to
CC fuse to uterine seam cells (anchor cell-block) (PubMed:9834196).
CC Animals also have defective vulval morphogenesis and gonad migration
CC with increased pi cell divisions, which thus leads to an improper
CC uterine-vulval connection (PubMed:9834196, PubMed:14668410). Defective
CC response to CO(2) sensing (PubMed:23671427).
CC {ECO:0000269|PubMed:14668410, ECO:0000269|PubMed:23671427}.
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DR EMBL; AF097319; AAD12252.1; -; mRNA.
DR EMBL; FO081738; CCD74407.1; -; Genomic_DNA.
DR EMBL; FO081738; CCD74408.1; -; Genomic_DNA.
DR EMBL; FO081738; CCD74409.1; -; Genomic_DNA.
DR EMBL; FO081738; CCD74410.1; -; Genomic_DNA.
DR PIR; T25909; T25909.
DR PIR; T43675; T43675.
DR RefSeq; NP_001024918.1; NM_001029747.3. [Q23045-1]
DR RefSeq; NP_001024920.1; NM_001029749.3.
DR RefSeq; NP_001294841.1; NM_001307912.1.
DR RefSeq; NP_001294842.1; NM_001307913.1. [Q23045-4]
DR AlphaFoldDB; Q23045; -.
DR SMR; Q23045; -.
DR IntAct; Q23045; 73.
DR STRING; 6239.T22B7.1a; -.
DR EPD; Q23045; -.
DR PaxDb; Q23045; -.
DR PeptideAtlas; Q23045; -.
DR PRIDE; Q23045; -.
DR EnsemblMetazoa; T22B7.1a.1; T22B7.1a.1; WBGene00001182. [Q23045-1]
DR EnsemblMetazoa; T22B7.1a.2; T22B7.1a.2; WBGene00001182. [Q23045-1]
DR EnsemblMetazoa; T22B7.1a.3; T22B7.1a.3; WBGene00001182. [Q23045-1]
DR EnsemblMetazoa; T22B7.1b.1; T22B7.1b.1; WBGene00001182. [Q23045-2]
DR EnsemblMetazoa; T22B7.1b.2; T22B7.1b.2; WBGene00001182. [Q23045-2]
DR EnsemblMetazoa; T22B7.1b.3; T22B7.1b.3; WBGene00001182. [Q23045-2]
DR EnsemblMetazoa; T22B7.1c.1; T22B7.1c.1; WBGene00001182. [Q23045-3]
DR EnsemblMetazoa; T22B7.1d.1; T22B7.1d.1; WBGene00001182. [Q23045-4]
DR EnsemblMetazoa; T22B7.1d.2; T22B7.1d.2; WBGene00001182. [Q23045-4]
DR GeneID; 180833; -.
DR KEGG; cel:CELE_T22B7.1; -.
DR UCSC; T22B7.1c.2; c. elegans.
DR CTD; 180833; -.
DR WormBase; T22B7.1a; CE28487; WBGene00001182; egl-13. [Q23045-1]
DR WormBase; T22B7.1b; CE33702; WBGene00001182; egl-13. [Q23045-2]
DR WormBase; T22B7.1c; CE38552; WBGene00001182; egl-13. [Q23045-3]
DR WormBase; T22B7.1d; CE05030; WBGene00001182; egl-13. [Q23045-4]
DR eggNOG; KOG0528; Eukaryota.
DR GeneTree; ENSGT00940000170160; -.
DR InParanoid; Q23045; -.
DR OMA; AQTEHGC; -.
DR OrthoDB; 1641977at2759; -.
DR Reactome; R-CEL-3769402; Deactivation of the beta-catenin transactivating complex.
DR SignaLink; Q23045; -.
DR PRO; PR:Q23045; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00001182; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR ExpressionAtlas; Q23045; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:WormBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IMP:WormBase.
DR GO; GO:0055059; P:asymmetric neuroblast division; IMP:UniProtKB.
DR GO; GO:0045165; P:cell fate commitment; IBA:GO_Central.
DR GO; GO:0001708; P:cell fate specification; IMP:WormBase.
DR GO; GO:0048664; P:neuron fate determination; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0040025; P:vulval development; IMP:WormBase.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR Pfam; PF00505; HMG_box; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Differentiation; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..470
FT /note="Transcription factor egl-13"
FT /evidence="ECO:0000305"
FT /id="PRO_0000432858"
FT DNA_BIND 329..397
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 1..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 294..328
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..31
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 59..79
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..321
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..157
FT /note="MSRRRKANPTKLSENAKKLAKEVENSQEENDCDMLAKPQTPTIIIPGHMDDT
FT PNPAGSPHDASIKSSSSTISDHTSTSATTGISDFPDILAQTEHGCSVLIDGNHLREIIN
FT SVDTQDGKQDLLSDVIRQLTSIKERLTNDESPVKDDLKEDPDDMSP -> MWPMVQ
FT (in isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_057620"
FT VAR_SEQ 1..154
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_057621"
FT VAR_SEQ 1..48
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_057622"
FT MUTAGEN 177..470
FT /note="Missing: In cas11; has extra A/PVM neurons but fewer
FT neurons in the Q.a lineage. In QL.ap neuroblasts, abolishes
FT expression of gcy-32, but ectopically expresses mec-4."
FT /evidence="ECO:0000269|PubMed:23946438"
FT MUTAGEN 331
FT /note="R->K: In ty7; egg laying defective phenotype."
FT /evidence="ECO:0000269|PubMed:14668410"
FT MUTAGEN 335
FT /note="A->T: In ku207; egg laying defective phenotype."
FT /evidence="ECO:0000269|PubMed:9834196"
FT MUTAGEN 364
FT /note="G->E: In rp26; egg laying defective phenotype."
FT /evidence="ECO:0000269|PubMed:23671427"
FT MUTAGEN 394
FT /note="P->L: In n483; increased uterine cell divisions."
FT /evidence="ECO:0000269|PubMed:14668410"
SQ SEQUENCE 470 AA; 52276 MW; 0D8A5DFF8E51C63E CRC64;
MSRRRKANPT KLSENAKKLA KEVENSQEEN DCDMLAKPQT PTIIIPGHMD DTPNPAGSPH
DASIKSSSST ISDHTSTSAT TGISDFPDIL AQTEHGCSVL IDGNHLREII NSVDTQDGKQ
DLLSDVIRQL TSIKERLTND ESPVKDDLKE DPDDMSPMLH AGNFDSEMLL RQHELMQHQQ
QQMIIANMLK ATQSLPLLFN GGLNYEAILN NPVLNATIAG HLPNPLASNI SLLQKSISAK
LAAAGNMQTV EKVETPLNLS KDTPSPTAIP QSPLSGFRLP YSLGTNYGSD GQLFNNCSPN
SSGKSTPGNT SVTSEVATPR PQAKSPNHIK RPMNAFMVWA RDERRKILKA YPDMHNSNIS
KILGSRWKGM SNSEKQPYYE EQSRLSKLHM EQHPDYRYRP RPKRTCVIDG KKVRVNEYKT
IMKTKKDLMW GDEPGFSQPS DLQMDLASHV NLLNDLTQHH HQSHLLQTAE
