EGL26_CAEEL
ID EGL26_CAEEL Reviewed; 317 AA.
AC P91082; A0A0K3AWT5;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Egg-laying defective protein 26 {ECO:0000312|WormBase:C23H3.1a};
DE AltName: Full=Putative acyltransferase egl-26 {ECO:0000305};
DE EC=2.3.1.- {ECO:0000305};
GN Name=egl-26 {ECO:0000303|PubMed:11784109, ECO:0000312|WormBase:C23H3.1a};
GN Synonyms=cog-4 {ECO:0000312|WormBase:C23H3.1a},
GN egl-48 {ECO:0000312|WormBase:C23H3.1a};
GN ORFNames=C23H3.1 {ECO:0000312|WormBase:C23H3.1a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND MUTAGENESIS OF GLY-150; GLY-168 AND SER-275.
RX PubMed=11784109; DOI=10.1006/dbio.2001.0514;
RA Hanna-Rose W., Han M.;
RT "The Caenorhabditis elegans EGL-26 protein mediates vulval cell
RT morphogenesis.";
RL Dev. Biol. 241:247-258(2002).
RN [3] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF HIS-166; GLY-168;
RP HIS-178; CYS-261 AND SER-275.
RX PubMed=17560977; DOI=10.1016/j.ydbio.2007.05.020;
RA Estes K.A., Kalamegham R., Hanna-Rose W.;
RT "Membrane localization of the NlpC/P60 family protein EGL-26 correlates
RT with regulation of vulval cell morphogenesis in Caenorhabditis elegans.";
RL Dev. Biol. 308:196-205(2007).
CC -!- FUNCTION: Putative acyltransferase (Probable). Plays a role in the
CC morphogenesis of a vulval toroid cell, vulF, which is located where the
CC vulva and the uterus connect (PubMed:11784109, PubMed:17560977). Not
CC required for specifying vulval cell fate (PubMed:17560977).
CC {ECO:0000269|PubMed:11784109, ECO:0000269|PubMed:17560977,
CC ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane
CC {ECO:0000269|PubMed:11784109, ECO:0000269|PubMed:17560977}; Peripheral
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:C23H3.1a};
CC IsoId=P91082-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:C23H3.1b};
CC IsoId=P91082-2; Sequence=VSP_061110;
CC -!- TISSUE SPECIFICITY: Highly expressed in the cells of the spermatheca,
CC the mouth, and the lining of the pharynx, the rectum, and the excretory
CC canal (PubMed:11784109). Also expressed in the pharyngeal intestinal
CC junction cell (PubMed:11784109). {ECO:0000269|PubMed:11784109}.
CC -!- DEVELOPMENTAL STAGE: During the L3 larval stage, transiently expressed
CC in the anchor cell, in rectal epithelial cells D, VL, and VR, in B and
CC in Y, and in several neuronal cells (PubMed:11784109). During the L4
CC larval stage, expressed near the vulva and the uterus, with expression
CC lining the lumen of the uterus and portions of the vulval lumen
CC including the vulval toroid cells vulB and vulE (PubMed:11784109).
CC During the L4 larval stage, not expressed in the vulval toroid vulF
CC (PubMed:11784109). {ECO:0000269|PubMed:11784109}.
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DR EMBL; BX284602; CCD65320.1; -; Genomic_DNA.
DR EMBL; BX284602; CTQ86451.1; -; Genomic_DNA.
DR PIR; T25560; T25560.
DR RefSeq; NP_001300512.1; NM_001313583.1. [P91082-2]
DR RefSeq; NP_493652.1; NM_061251.3. [P91082-1]
DR AlphaFoldDB; P91082; -.
DR IntAct; P91082; 2.
DR STRING; 6239.C23H3.1; -.
DR PaxDb; P91082; -.
DR PeptideAtlas; P91082; -.
DR EnsemblMetazoa; C23H3.1a.1; C23H3.1a.1; WBGene00001193. [P91082-1]
DR EnsemblMetazoa; C23H3.1b.1; C23H3.1b.1; WBGene00001193. [P91082-2]
DR GeneID; 173392; -.
DR KEGG; cel:CELE_C23H3.1; -.
DR UCSC; C23H3.1; c. elegans. [P91082-1]
DR CTD; 173392; -.
DR WormBase; C23H3.1a; CE08320; WBGene00001193; egl-26. [P91082-1]
DR WormBase; C23H3.1b; CE50533; WBGene00001193; egl-26. [P91082-2]
DR eggNOG; ENOG502R7CS; Eukaryota.
DR HOGENOM; CLU_877803_0_0_1; -.
DR InParanoid; P91082; -.
DR OMA; ASHPLIC; -.
DR OrthoDB; 952678at2759; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00001193; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR ExpressionAtlas; P91082; baseline and differential.
DR GO; GO:0016324; C:apical plasma membrane; IDA:WormBase.
DR GO; GO:0098592; C:cytoplasmic side of apical plasma membrane; IDA:UniProtKB.
DR GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0000902; P:cell morphogenesis; IMP:WormBase.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0002119; P:nematode larval development; IMP:WormBase.
DR GO; GO:0018991; P:oviposition; IMP:WormBase.
DR GO; GO:0040026; P:positive regulation of vulval development; IMP:UniProtKB.
DR GO; GO:0040025; P:vulval development; IMP:WormBase.
DR InterPro; IPR007053; LRAT_dom.
DR Pfam; PF04970; LRAT; 1.
DR PROSITE; PS51934; LRAT; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Alternative splicing; Cell membrane; Membrane;
KW Reference proteome; Transferase.
FT CHAIN 1..317
FT /note="Egg-laying defective protein 26"
FT /id="PRO_0000453173"
FT DOMAIN 156..277
FT /note="LRAT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01283"
FT ACT_SITE 166
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01283"
FT ACT_SITE 178
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01283"
FT ACT_SITE 261
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01283"
FT VAR_SEQ 185
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_061110"
FT MUTAGEN 150
FT /note="G->E: In ku228 and e1952; vulval morphology defects
FT displaying a connection of gonad defective (Cog) phenotype
FT where there is failure to make a proper connection between
FT the vulval and uterine lumens. This is caused by thick
FT disorganized tissue at the apex of the vulva which blocks
FT the exit to the vulva from the uterus. Abnormal vulF cell
FT morphology and irregular positioning of vulF nuclei at the
FT apex of the vulva. These defects most likely cause an egg-
FT laying defective (Egl) phenotype where progeny hatch in the
FT uterus killing the mother."
FT /evidence="ECO:0000269|PubMed:11784109"
FT MUTAGEN 166
FT /note="H->A: Does not rescue the egg-laying defect of the
FT egl-26 ku211 mutant."
FT /evidence="ECO:0000269|PubMed:17560977"
FT MUTAGEN 168
FT /note="G->E: In ku211; vulval morphology defects displaying
FT a connection of gonad defective (Cog) phenotype where there
FT is failure to make a proper connection between the vulval
FT and uterine lumens. This is caused by thick disorganized
FT tissue at the apex of the vulva which blocks the exit to
FT the vulva from the uterus. Abnormal vulF cell morphology
FT and irregular positioning of vulF nuclei at the apex of the
FT vulva. These defects most likely cause an egg-laying
FT defective (Egl) phenotype where progeny hatch in the uterus
FT killing the mother. No anchor cell invasion or fusion
FT defects during the L3 and L4 larval stages."
FT /evidence="ECO:0000269|PubMed:11784109,
FT ECO:0000269|PubMed:17560977"
FT MUTAGEN 178
FT /note="H->A: Rescues the egg-laying defect of the egl-26
FT ku211 mutant."
FT /evidence="ECO:0000269|PubMed:17560977"
FT MUTAGEN 261
FT /note="C->A: Does not rescue the egg-laying defect of the
FT egl-26 ku211 mutant."
FT /evidence="ECO:0000269|PubMed:17560977"
FT MUTAGEN 275
FT /note="S->A,T: Localizes to the apical cell membrane, but
FT is abundant in the cytoplasm. Rescues the egg-laying defect
FT of the egl-26 ku211 mutant."
FT /evidence="ECO:0000269|PubMed:17560977"
FT MUTAGEN 275
FT /note="S->E: Localizes to the cytoplasm rather than to the
FT apical cell membrane. Does not rescue the egg-laying defect
FT of the egl-26 ku211 mutant."
FT /evidence="ECO:0000269|PubMed:17560977"
FT MUTAGEN 275
FT /note="S->F: In n481; vulval morphology defects displaying
FT a connection of gonad defective (Cog) phenotype where there
FT is failure to make a proper connection between the vulval
FT and uterine lumens. This is caused by thick disorganized
FT tissue at the apex of the vulva which blocks the exit to
FT the vulva from the uterus. Abnormal vulF cell morphology
FT and irregular positioning of vulF nuclei at the apex of the
FT vulva. These defects most likely cause an egg-laying
FT defective (Egl) phenotype where progeny hatch in the uterus
FT killing the mother. Localizes to the cytoplasm rather than
FT to the apical cell membrane. Rescues the egg-laying defect
FT of the egl-26 ku211 mutant."
FT /evidence="ECO:0000269|PubMed:11784109,
FT ECO:0000269|PubMed:17560977"
SQ SEQUENCE 317 AA; 35164 MW; FB3B9588F4EE95D7 CRC64;
MLTCIQPSSS SIGGFGKADD NVRILLMASV RNEFGDTSIF SRLGVTALGQ HYVLVTKKKM
FGGYTTHMIT ANMRNRPFIS IPFKVSSGAQ SIEESRDLIS RLTTVLGRPG MFSFDDPPIG
SQFPVGKELI QLDEVPVGVH DRQDKYLEKG DEVFCEVNVS GVKFYHSGIY AGDGMCYHFV
CDAQESESFA DALAVFSGAS AHVVYDTWFE FVYALVEVSD VPPKIFRASH PLICRSGEQV
VKYAEHLQRE LENYDIRRCN CQHFSSECST GVPFSYDMTS NFKYLACTVL KPTSTVVNAM
TRPNRDRSSF ASSSTSS
