EGL44_CAEEL
ID EGL44_CAEEL Reviewed; 486 AA.
AC Q19849; D7SFJ5; D7SFJ6; D7SFJ7; D7SFJ8; D7SFJ9; Q86NH3; Q86NH4; Q86NH5;
AC Q86NH6; Q95QI4; Q9GT31;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 4.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Transcription enhancer factor-like protein egl-44;
DE AltName: Full=Egg-laying defective protein 44;
GN Name=egl-44; ORFNames=F28B12.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF ALA-134 AND ARG-140,
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=11274062; DOI=10.1101/gad.857401;
RA Wu J., Duggan A., Chalfie M.;
RT "Inhibition of touch cell fate by egl-44 and egl-46 in C. elegans.";
RL Genes Dev. 15:789-802(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF
RP ALA-134.
RX PubMed=12954713; DOI=10.1242/dev.00678;
RA Yu H., Pretot R.F., Burglin T.R., Sternberg P.W.;
RT "Distinct roles of transcription factors EGL-46 and DAF-19 in specifying
RT the functionality of a polycystin-expressing sensory neuron necessary for
RT C. elegans male vulva location behavior.";
RL Development 130:5217-5227(2003).
RN [4]
RP FUNCTION, INTERACTION WITH EGL-46, AND MUTAGENESIS OF ALA-91; ALA-134;
RP ARG-140 AND ARG-145.
RX PubMed=23946438; DOI=10.1242/dev.098723;
RA Feng G., Yi P., Yang Y., Chai Y., Tian D., Zhu Z., Liu J., Zhou F.,
RA Cheng Z., Wang X., Li W., Ou G.;
RT "Developmental stage-dependent transcriptional regulatory pathways control
RT neuroblast lineage progression.";
RL Development 140:3838-3847(2013).
RN [5]
RP FUNCTION, AND INTERACTION WITH YAP-1.
RX PubMed=23396260; DOI=10.1016/j.yexcr.2013.01.020;
RA Iwasa H., Maimaiti S., Kuroyanagi H., Kawano S., Inami K., Timalsina S.,
RA Ikeda M., Nakagawa K., Hata Y.;
RT "Yes-associated protein homolog, YAP-1, is involved in the thermotolerance
RT and aging in the nematode Caenorhabditis elegans.";
RL Exp. Cell Res. 319:931-945(2013).
RN [6]
RP FUNCTION.
RX PubMed=29031632; DOI=10.1016/j.ydbio.2017.10.009;
RA O'Brien B.M.J., Palumbos S.D., Novakovic M., Shang X., Sundararajan L.,
RA Miller D.M. III;
RT "Separate transcriptionally regulated pathways specify distinct classes of
RT sister dendrites in a nociceptive neuron.";
RL Dev. Biol. 432:248-257(2017).
RN [7]
RP FUNCTION, AND INTERACTION WITH EGL-46.
RX PubMed=30291162; DOI=10.1242/dev.168096;
RA Zheng C., Jin F.Q., Trippe B.L., Wu J., Chalfie M.;
RT "Inhibition of cell fate repressors secures the differentiation of the
RT touch receptor neurons of Caenorhabditis elegans.";
RL Development 145:0-0(2018).
CC -!- FUNCTION: Transcription factor (PubMed:30291162). Binds to DNA sequence
CC motif 5'-CATNNNNAAATGCAT-3' as a heterodimer with egl-46
CC (PubMed:30291162). Represses expression of genes involved in
CC differentiation of touch receptor neurons (TRN), probably acting as a
CC heterodimer with egl-46, perhaps by occupying similar cis-regulatory
CC elements as an unc-86/mec-3 heterodimer (PubMed:30291162). Plays a role
CC in cell fate specification of neurons, including the hook neuron HOB,
CC and touch receptor neurons (PubMed:12954713, PubMed:30291162). Involved
CC in male mating behavior, acting in concert with egl-46, via modulation
CC of expression of polycystins lov-1 and pkd-2, homeodomain protein ceh-
CC 26, and neuropeptide-like protein nlp-8 (PubMed:12954713). Acts
CC upstream of egl-46 to prevent touch cell differentiation in FLP neurons
CC (PubMed:11274062). Plays a role in neuron differentiation by repressing
CC the expression of zag-1 in FLP neurons, probably acting as a
CC heterodimer with egl-46; because zag-1 represses expression of egl-46
CC and egl-44, together these proteins form a bistable, negative-feedback
CC loop that regulates the choice between neuronal fates
CC (PubMed:30291162). Also promotes HSN neuron development
CC (PubMed:11274062). In association with egl-46, regulates cell cycle
CC exit in the neuronal Q cell lineage (PubMed:23946438). Plays a role in
CC specifying commissural dendrites of the PVD nociceptive neurons, acting
CC in concert with egl-46 (PubMed:29031632). May be involved in thermal
CC stress response downstream of yap-1 (PubMed:23396260).
CC {ECO:0000269|PubMed:11274062, ECO:0000269|PubMed:12954713,
CC ECO:0000269|PubMed:23946438, ECO:0000269|PubMed:29031632,
CC ECO:0000269|PubMed:30291162, ECO:0000303|PubMed:23396260}.
CC -!- SUBUNIT: Interacts (via N-terminus) with egl-46 (via C-terminus); the
CC interaction is direct; the interaction may regulate transcription
CC (PubMed:23946438, PubMed:30291162). Interacts with yap-1 (via WW
CC domain); the interaction may regulate transcription (PubMed:23396260).
CC {ECO:0000269|PubMed:23396260, ECO:0000269|PubMed:23946438,
CC ECO:0000269|PubMed:30291162}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11274062}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=a;
CC IsoId=Q19849-2; Sequence=Displayed;
CC Name=b;
CC IsoId=Q19849-1; Sequence=VSP_041803, VSP_041804;
CC Name=c;
CC IsoId=Q19849-3; Sequence=VSP_041803;
CC Name=d;
CC IsoId=Q19849-4; Sequence=VSP_041801;
CC Name=f;
CC IsoId=Q19849-6; Sequence=VSP_041802, VSP_041804;
CC -!- TISSUE SPECIFICITY: Expressed in HSN neurons in embryos and in the FLP
CC neurons from the L1 stage through to adults. Not expressed in touch
CC cells. Also expressed in larval hypodermis, intestine, pharyngeal
CC muscle and other neurons. In adults expression is lost from some
CC neurons, is weaker in the hypodermis but remains in the intestine
CC (PubMed:11274062). Expressed in HOB neuron, ray neurons RnA and RnB,
CC and the ray structural cell, Rnst; rays are male-specific genital
CC sensilla (simple sense organs) (PubMed:12954713).
CC {ECO:0000269|PubMed:11274062, ECO:0000269|PubMed:12954713}.
CC -!- DEVELOPMENTAL STAGE: Expressed from before gastrulation through to
CC adult (PubMed:11274062). Expressed in HOB, PVX, and PVY neurons
CC (PubMed:12954713). Expressed in most descendants of the ray precursor
CC cells (Rn), including the ray neurons (RnA and RnB) and the ray
CC structure cells at the L4 larval stage; rays are male-specific genital
CC sensilla (simple sense organs) (PubMed:12954713).
CC {ECO:0000269|PubMed:11274062, ECO:0000269|PubMed:12954713}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG13397.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF283982; AAG13397.1; ALT_SEQ; Genomic_DNA.
DR EMBL; FO080105; CCD61238.1; -; Genomic_DNA.
DR EMBL; FO080105; CCD61239.1; -; Genomic_DNA.
DR EMBL; FO080105; CCD61240.1; -; Genomic_DNA.
DR EMBL; FO080105; CCD61241.1; -; Genomic_DNA.
DR EMBL; FO080105; CCD61242.1; -; Genomic_DNA.
DR RefSeq; NP_495186.3; NM_062785.3. [Q19849-3]
DR RefSeq; NP_741006.3; NM_171007.3. [Q19849-1]
DR RefSeq; NP_741007.2; NM_171850.4. [Q19849-2]
DR RefSeq; NP_871904.2; NM_182104.3.
DR RefSeq; NP_871906.2; NM_182106.4.
DR AlphaFoldDB; Q19849; -.
DR SMR; Q19849; -.
DR BioGRID; 39346; 6.
DR IntAct; Q19849; 4.
DR STRING; 6239.F28B12.2a; -.
DR EPD; Q19849; -.
DR PaxDb; Q19849; -.
DR PeptideAtlas; Q19849; -.
DR EnsemblMetazoa; F28B12.2a.1; F28B12.2a.1; WBGene00001208. [Q19849-2]
DR EnsemblMetazoa; F28B12.2b.1; F28B12.2b.1; WBGene00001208. [Q19849-1]
DR EnsemblMetazoa; F28B12.2b.2; F28B12.2b.2; WBGene00001208. [Q19849-1]
DR EnsemblMetazoa; F28B12.2b.3; F28B12.2b.3; WBGene00001208. [Q19849-1]
DR EnsemblMetazoa; F28B12.2c.1; F28B12.2c.1; WBGene00001208. [Q19849-3]
DR EnsemblMetazoa; F28B12.2c.2; F28B12.2c.2; WBGene00001208. [Q19849-3]
DR EnsemblMetazoa; F28B12.2d.1; F28B12.2d.1; WBGene00001208. [Q19849-4]
DR EnsemblMetazoa; F28B12.2f.1; F28B12.2f.1; WBGene00001208. [Q19849-6]
DR GeneID; 174005; -.
DR UCSC; F28B12.2a; c. elegans. [Q19849-1]
DR CTD; 174005; -.
DR WormBase; F28B12.2a; CE44866; WBGene00001208; egl-44. [Q19849-2]
DR WormBase; F28B12.2b; CE45037; WBGene00001208; egl-44. [Q19849-1]
DR WormBase; F28B12.2c; CE44843; WBGene00001208; egl-44. [Q19849-3]
DR WormBase; F28B12.2d; CE45004; WBGene00001208; egl-44. [Q19849-4]
DR WormBase; F28B12.2f; CE44907; WBGene00001208; egl-44. [Q19849-6]
DR eggNOG; KOG3841; Eukaryota.
DR GeneTree; ENSGT00950000182956; -.
DR InParanoid; Q19849; -.
DR OMA; AISGFWQ; -.
DR PhylomeDB; Q19849; -.
DR Reactome; R-CEL-2032785; YAP1- and WWTR1 (TAZ)-stimulated gene expression.
DR Reactome; R-CEL-8951671; RUNX3 regulates YAP1-mediated transcription.
DR SignaLink; Q19849; -.
DR PRO; PR:Q19849; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00001208; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR ExpressionAtlas; Q19849; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IPI:WormBase.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:WormBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IMP:UniProtKB.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IPI:UniProtKB.
DR GO; GO:0001223; F:transcription coactivator binding; IPI:WormBase.
DR GO; GO:0055059; P:asymmetric neuroblast division; IMP:UniProtKB.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0048568; P:embryonic organ development; IBA:GO_Central.
DR GO; GO:0035329; P:hippo signaling; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; IMP:WormBase.
DR GO; GO:0031536; P:positive regulation of exit from mitosis; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IGI:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IGI:UniProtKB.
DR Gene3D; 6.10.20.40; -; 1.
DR InterPro; IPR000818; TEA/ATTS_dom.
DR InterPro; IPR038096; TEA/ATTS_sf.
DR InterPro; IPR016361; TEF_metazoa.
DR InterPro; IPR041086; YBD.
DR Pfam; PF01285; TEA; 1.
DR Pfam; PF17725; YBD; 1.
DR PIRSF; PIRSF002603; TEF; 1.
DR PRINTS; PR00065; TEADOMAIN.
DR SMART; SM00426; TEA; 1.
DR PROSITE; PS00554; TEA_1; 1.
DR PROSITE; PS51088; TEA_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Developmental protein; Differentiation;
KW DNA-binding; Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..486
FT /note="Transcription enhancer factor-like protein egl-44"
FT /id="PRO_0000205944"
FT DNA_BIND 88..164
FT /note="TEA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00505"
FT REGION 47..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 165..188
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 47..61
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..75
FT /note="Missing (in isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_041801"
FT VAR_SEQ 1..58
FT /note="Missing (in isoform f)"
FT /evidence="ECO:0000305"
FT /id="VSP_041802"
FT VAR_SEQ 1..15
FT /note="Missing (in isoform b and isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_041803"
FT VAR_SEQ 170
FT /note="K -> KVPFHLNT (in isoform b and isoform f)"
FT /evidence="ECO:0000305"
FT /id="VSP_041804"
FT MUTAGEN 91
FT /note="A->V: In cas140; production of an extra neuron from
FT the Q lineage and a mild egg-laying defect."
FT /evidence="ECO:0000269|PubMed:23946438"
FT MUTAGEN 134
FT /note="A->V: In n1080 and cas58; two-fold reduction in mRNA
FT levels. Production of an extra neuron from the Q lineage
FT and a mild egg-laying defect. Decreased mating efficiency
FT in males, as a result of deficiency in vulval location.
FT Significant decrease in expression of ceh-26 and lov-1 in
FT HOB neuron, and some reduction in expression of pkd-2 and
FT nlp-8."
FT /evidence="ECO:0000269|PubMed:11274062,
FT ECO:0000269|PubMed:12954713, ECO:0000269|PubMed:23946438"
FT MUTAGEN 140
FT /note="R->Q: In n998, n1087 and cas6; two-fold reduction in
FT mRNA levels. Production of an extra neuron from the Q
FT lineage and a mild egg-laying defect."
FT /evidence="ECO:0000269|PubMed:11274062,
FT ECO:0000269|PubMed:23946438"
FT MUTAGEN 145
FT /note="R->K: In cas3; production of an extra neuron from
FT the Q lineage and a mild egg-laying defect."
FT /evidence="ECO:0000269|PubMed:23946438"
SQ SEQUENCE 486 AA; 53692 MW; 4584AA0B129FCA7A CRC64;
MNSMFCSELQ QALPQMSEDV AVTAQTILNG TPPHNHFRKE WLAGAAGTTT PTTTSGGQMM
TLSPPAGDGP GSAGSMAPES TSSLSDLSGD AEGVWSIDID QAFQEALAIY PPCGRRKIII
SDEGKMYGRN ELIARYIKLR CGKTRTRKQV SSHIQVLARK KLRDEQAKKK GDIPSLLQQA
SPPGGVKSPS AVVFPPVSAA VAAITEISPQ SSYSSIVPKV ETDQISQQLF KSLPLWSFQQ
TPGLPIGMDL SQLVFQQSSP DKTVSPVKSE VVEETKPIAS SQLTLHSFSA YVKCNKTSLR
TELVKIENTL EKDDIDISVF YEKYPKLLRE LFEKSEKKDV FFLAKCWANI NVSDDVQNCQ
YAVDSFYSSR EKFQLKVSTM ACSFGNQAVE KIEQYFPIEF DGSYSFILNN SPMCDYMVKF
IAELKKLNVI ETMNNVLENF TVLQIVTNSE TDELLMVLCF VFEVSQEPEP SCSVYRLIDG
GGDSDE
