EGL5_CAEEL
ID EGL5_CAEEL Reviewed; 223 AA.
AC P17486; Q21061; Q7YZV2;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2005, sequence version 4.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Homeobox protein egl-5 {ECO:0000305};
DE AltName: Full=Abdominal-B homolog egl-5 {ECO:0000303|PubMed:17574230, ECO:0000303|PubMed:8101474};
DE AltName: Full=Egg-laying defective protein 5 {ECO:0000312|WormBase:C08C3.1b};
DE AltName: Full=Homeobox protein ceh-11 {ECO:0000312|WormBase:C08C3.1b};
GN Name=egl-5 {ECO:0000312|WormBase:C08C3.1b};
GN Synonyms=ceh-11 {ECO:0000312|WormBase:C08C3.1b};
GN ORFNames=C08C3.1 {ECO:0000312|WormBase:C08C3.1b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, DEVELOPMENTAL STAGE, AND
RP MUTAGENESIS OF ARG-163.
RX PubMed=8101474; DOI=10.1016/0092-8674(93)90292-x;
RA Wang B.B., Mueller-Immergluck M.M., Austin J., Robinson N.T.,
RA Chisholm A.D., Kenyon C.;
RT "A homeotic gene cluster patterns the anteroposterior body axis of C.
RT elegans.";
RL Cell 74:29-42(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [3] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 111-185.
RX PubMed=1970877; DOI=10.1093/nar/18.8.2033;
RA Schaller D., Wittmann C., Spicher A., Mueller F., Tobler H.;
RT "Cloning and analysis of three new homeobox genes from the nematode
RT Caenorhabditis elegans.";
RL Nucleic Acids Res. 18:2033-2036(1990).
RN [5]
RP MUTAGENESIS OF ARG-163.
RX PubMed=2721931; DOI=10.1093/genetics/121.4.703;
RA Desai C., Horvitz H.R.;
RT "Caenorhabditis elegans mutants defective in the functioning of the motor
RT neurons responsible for egg laying.";
RL Genetics 121:703-721(1989).
RN [6]
RP FUNCTION, AND MUTAGENESIS OF ARG-163.
RX PubMed=1879361; DOI=10.1242/dev.111.4.921;
RA Chisholm A.;
RT "Control of cell fate in the tail region of C. elegans by the gene egl-5.";
RL Development 111:921-932(1991).
RN [7]
RP FUNCTION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF ARG-163.
RX PubMed=10049576; DOI=10.1006/dbio.1998.9124;
RA Ferreira H.B., Zhang Y., Zhao C., Emmons S.W.;
RT "Patterning of Caenorhabditis elegans posterior structures by the
RT Abdominal-B homolog, egl-5.";
RL Dev. Biol. 207:215-228(1999).
RN [8]
RP FUNCTION, AND MUTAGENESIS OF ARG-163.
RX PubMed=12835398; DOI=10.1242/dev.00398;
RA Toker A.S., Teng Y., Ferreira H.B., Emmons S.W., Chalfie M.;
RT "The Caenorhabditis elegans spalt-like gene sem-4 restricts touch cell fate
RT by repressing the selector Hox gene egl-5 and the effector gene mec-3.";
RL Development 130:3831-3840(2003).
RN [9]
RP DEVELOPMENTAL STAGE.
RX PubMed=17574230; DOI=10.1016/j.ydbio.2007.05.021;
RA Deng H., Sun Y., Zhang Y., Luo X., Hou W., Yan L., Chen Y., Tian E.,
RA Han J., Zhang H.;
RT "Transcription factor NFY globally represses the expression of the C.
RT elegans Hox gene Abdominal-B homolog egl-5.";
RL Dev. Biol. 308:583-592(2007).
RN [10]
RP FUNCTION.
RX PubMed=18505863; DOI=10.1534/genetics.108.088948;
RA Singhvi A., Frank C.A., Garriga G.;
RT "The T-box gene tbx-2, the homeobox gene egl-5 and the asymmetric cell
RT division gene ham-1 specify neural fate in the HSN/PHB lineage.";
RL Genetics 179:887-898(2008).
RN [11]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19232338; DOI=10.1016/j.ydbio.2009.01.044;
RA Nicholas H.R., Hodgkin J.;
RT "The C. elegans Hox gene egl-5 is required for correct development of the
RT hermaphrodite hindgut and for the response to rectal infection by
RT Microbacterium nematophilum.";
RL Dev. Biol. 329:16-24(2009).
RN [12]
RP FUNCTION, INTERACTION WITH POP-1, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF
RP ARG-163.
RX PubMed=20553900; DOI=10.1016/j.ydbio.2010.05.516;
RA Kalis A.K., Murphy M.W., Zarkower D.;
RT "EGL-5/ABD-B plays an instructive role in male cell fate determination in
RT the C. elegans somatic gonad.";
RL Dev. Biol. 344:827-835(2010).
CC -!- FUNCTION: Involved in control of cell fate and pattern formation along
CC the anterior-posterior axis, acting mainly in the tail (PubMed:8101474,
CC PubMed:1879361, PubMed:10049576). Required during embryonic and
CC postembryonic development (PubMed:1879361). Essential for the
CC determination of specific neurons, including the PLM touch neurons
CC (PubMed:8101474, PubMed:12835398). Plays a role in neural fate
CC specification in the hermaphrodite-specific neuron (HSN)/PHB neuron
CC lineage, acting in concert with T-box protein tbx-2 and the asymmetric
CC cell division protein ham-1 (PubMed:18505863). Required for male
CC gonadal fate determination, acting in parallel with a WNT/beta-catenin
CC pathway, perhaps by recruiting pop-1 to male-specific gonadal target
CC genes (PubMed:20553900). Involved in development of the hermaphrodite
CC hindgut, and for the response to rectal infection by the coryneform
CC bacterium M.nematophilum (PubMed:19232338).
CC {ECO:0000269|PubMed:10049576, ECO:0000269|PubMed:12835398,
CC ECO:0000269|PubMed:18505863, ECO:0000269|PubMed:1879361,
CC ECO:0000269|PubMed:19232338, ECO:0000269|PubMed:20553900,
CC ECO:0000269|PubMed:8101474}.
CC -!- SUBUNIT: Interacts with the TCF transcription factor pop-1.
CC {ECO:0000269|PubMed:20553900}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10049576}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=b {ECO:0000312|WormBase:C08C3.1b};
CC IsoId=P17486-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:C08C3.1a};
CC IsoId=P17486-2; Sequence=VSP_015689;
CC Name=c {ECO:0000312|WormBase:C08C3.1c};
CC IsoId=P17486-3; Sequence=VSP_015688;
CC -!- DEVELOPMENTAL STAGE: Expressed throughout postembryonic development and
CC adulthood (at protein level) (PubMed:10049576). In young larvae (10 h
CC posthatching), expressed in five rectal epithelial cells, three left-
CC right pairs of neurons, and four to six bilateral pairs of body-wall
CC muscle cells, in the posterior body region (at protein level)
CC (PubMed:10049576). At the same developmental stage, also expressed in
CC the pair of hermaphrodite-specific neurons (HSN), in the mid-body
CC adjacent to the gonad (at protein level) (PubMed:10049576). In early
CC larvae, expressed in the male tail, and in hermaphrodite Ll stage
CC larvae (0-2.5 hr post-hatching) in the most posterior body region and
CC the tail (PubMed:17574230, PubMed:8101474). Expressed in most if not
CC all somatic cells in the male gonad, with the exception of the linker
CC cell (LC) and distal tip cells (DTCs) and their progenitors
CC (PubMed:20553900). {ECO:0000269|PubMed:10049576,
CC ECO:0000269|PubMed:17574230, ECO:0000269|PubMed:20553900,
CC ECO:0000269|PubMed:8101474}.
CC -!- DISRUPTION PHENOTYPE: Fails to swell on exposure to the rectal pathogen
CC M.nematophilum, a coryneform bacterium. {ECO:0000269|PubMed:19232338}.
CC -!- SIMILARITY: Belongs to the Abd-B homeobox family. {ECO:0000305}.
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DR EMBL; L19247; AAC37166.1; -; mRNA.
DR EMBL; BX284603; CCD63638.1; -; Genomic_DNA.
DR EMBL; BX284603; CCD63639.1; -; Genomic_DNA.
DR EMBL; BX284603; CCD63640.1; -; Genomic_DNA.
DR EMBL; X17075; CAA34927.1; -; Genomic_DNA.
DR PIR; A40722; A40722.
DR PIR; S44613; S44613.
DR RefSeq; NP_001021166.1; NM_001025995.2. [P17486-1]
DR RefSeq; NP_001021167.1; NM_001025996.1. [P17486-3]
DR RefSeq; NP_498697.3; NM_066296.4. [P17486-2]
DR AlphaFoldDB; P17486; -.
DR SMR; P17486; -.
DR BioGRID; 41301; 2.
DR STRING; 6239.C08C3.1b; -.
DR EPD; P17486; -.
DR PaxDb; P17486; -.
DR EnsemblMetazoa; C08C3.1a.1; C08C3.1a.1; WBGene00001174. [P17486-2]
DR EnsemblMetazoa; C08C3.1b.1; C08C3.1b.1; WBGene00001174. [P17486-1]
DR EnsemblMetazoa; C08C3.1c.1; C08C3.1c.1; WBGene00001174. [P17486-3]
DR GeneID; 176093; -.
DR KEGG; cel:CELE_C08C3.1; -.
DR UCSC; C08C3.1b; c. elegans. [P17486-1]
DR CTD; 176093; -.
DR WormBase; C08C3.1a; CE29076; WBGene00001174; egl-5. [P17486-2]
DR WormBase; C08C3.1b; CE32792; WBGene00001174; egl-5. [P17486-1]
DR WormBase; C08C3.1c; CE34523; WBGene00001174; egl-5. [P17486-3]
DR eggNOG; KOG0489; Eukaryota.
DR GeneTree; ENSGT00940000175004; -.
DR InParanoid; P17486; -.
DR OMA; PHWPNYA; -.
DR OrthoDB; 1349896at2759; -.
DR PhylomeDB; P17486; -.
DR SignaLink; P17486; -.
DR PRO; PR:P17486; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00001174; Expressed in embryo and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IPI:WormBase.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:UniProtKB.
DR GO; GO:0001708; P:cell fate specification; IMP:UniProtKB.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0061525; P:hindgut development; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0045138; P:nematode male tail tip morphogenesis; IMP:WormBase.
DR GO; GO:0001764; P:neuron migration; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0030155; P:regulation of cell adhesion; IMP:WormBase.
DR GO; GO:0043405; P:regulation of MAP kinase activity; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; DNA-binding; Homeobox;
KW Nucleus; Reference proteome.
FT CHAIN 1..223
FT /note="Homeobox protein egl-5"
FT /id="PRO_0000048865"
FT DNA_BIND 112..171
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 168..191
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..31
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 63..64
FT /note="GI -> V (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_015688"
FT VAR_SEQ 106..109
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_015689"
FT MUTAGEN 163
FT /note="R->C: In n486; egg-laying defects. Hermaphrodite-
FT specific neurons (HSN) are absent or misplaced. Males do
FT not produce progeny and often display gross abnormalities
FT of tail morphology. Abnormal development of the male-
FT specific genital sensilla (simple sense organs), known as
FT rays. Defects in rectal epithelium, including, in males,
FT absent or grossly abnormal spicules, gubernaculum, and
FT proctodeal chamber and, in both males and hermaphrodites,
FT defects in defecation. Insensitive to both light and heavy
FT touch in the tail. Alters the fates of PLM touch neurons;
FT substantially reduces expression of mec-3 and causes
FT abnormal morphology. Defects in the posterior ventral
FT neuroectoderm. Male somatic gonad is grossly abnormal and
FT exhibits gene expression typical of adult hermaphrodite
FT gonad, but there are no obvious gonadal defects in the
FT hermaphrodite."
FT /evidence="ECO:0000269|PubMed:10049576,
FT ECO:0000269|PubMed:12835398, ECO:0000269|PubMed:1879361,
FT ECO:0000269|PubMed:20553900, ECO:0000269|PubMed:2721931,
FT ECO:0000269|PubMed:8101474"
SQ SEQUENCE 223 AA; 25144 MW; F6343E9B0229CEDC CRC64;
MNTSTSAFDF GSSTASSAAT STTSSQPDAN DHLSRLAAMT QGVGKEDPET SSTPSTEASL
YPGISAAYMQ SYGWPQNYNY FGQPLGPATF PGWPQCYPNT AWPNYGELFA SSKKGRQTYQ
RYQTSVLEAK FQQSSYVSKK QREELRLQTQ LTDRQIKIWF QNRRMKAKKE KQRVDDHTEH
TPLLPANPPK GMGMDMDDEK KWQMAHWPPA AAHNPYQYPL CPP
