ID   AFPJ_SOLTU              Reviewed;          20 AA.
AC   P84794;
DT   18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   25-MAY-2022, entry version 24.
DE   RecName: Full=Antifungal protein J;
DE            Short=AFP-J;
DE   Flags: Fragment;
OS   Solanum tuberosum (Potato).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=4113;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   STRAIN=cv. Jopung {ECO:0000269|PubMed:16076139};
RC   TISSUE=Tuber {ECO:0000269|PubMed:16076139};
RX   PubMed=16076139; DOI=10.1021/jf0505123;
RA   Park Y., Choi B.H., Kwak J.-S., Kang C.-W., Lim H.-T., Cheong H.-S.,
RA   Hahm K.-S.;
RT   "Kunitz-type serine protease inhibitor from potato (Solanum tuberosum L.
RT   cv. Jopung).";
RL   J. Agric. Food Chem. 53:6491-6496(2005).
CC   -!- FUNCTION: Inhibitor of serine proteases chymotrypsin, pepsin and
CC       trypsin. Has strong antifungal activity against the human pathogenic
CC       fungi C.albicans TIMM 1768, S.cerevisiae KCTC 7296 and T.beigelli KCTC
CC       7707, but lacks antifungal activity against the plant pathogenic fungi
CC       C.gloeosporioides KACC 40003, C.coccodes KACC 40803 and D.bryoniae KACC
CC       40669. Lacks hemolytic activity against human erythrocytes.
CC       {ECO:0000269|PubMed:16076139}.
CC   -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000250|UniProtKB:P30941}.
CC   -!- MASS SPECTROMETRY: Mass=13500.5; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:16076139};
CC   -!- SIMILARITY: Belongs to the protease inhibitor I3 (leguminous Kunitz-
CC       type inhibitor) family. {ECO:0000305}.
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DR   AlphaFoldDB; P84794; -.
DR   Proteomes; UP000011115; Unassembled WGS sequence.
DR   GO; GO:0005773; C:vacuole; ISS:UniProtKB.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Fungicide; Protease inhibitor;
KW   Reference proteome; Serine protease inhibitor; Vacuole.
FT   CHAIN           1..>20
FT                   /note="Antifungal protein J"
FT                   /evidence="ECO:0000269|PubMed:16076139"
FT                   /id="PRO_0000232424"
FT   NON_TER         20
FT                   /evidence="ECO:0000303|PubMed:16076139"
SQ   SEQUENCE   20 AA;  2155 MW;  4ADCA154F3BB2F6C CRC64;
     LPSDATLVLD QTGKELDARL