ID   AFPR2_CUCMA             Reviewed;          15 AA.
AC   P86796;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2011, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Antifungal protein Pr-2 {ECO:0000303|PubMed:19807165};
DE   Flags: Fragment;
OS   Cucurbita maxima (Pumpkin) (Winter squash).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Cucurbiteae; Cucurbita.
OX   NCBI_TaxID=3661;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Peelings {ECO:0000269|PubMed:19807165};
RX   PubMed=19807165; DOI=10.1021/jf902005g;
RA   Park S.C., Kim J.Y., Lee J.K., Hwang I., Cheong H., Nah J.W., Hahm K.S.,
RA   Park Y.;
RT   "Antifungal mechanism of a novel antifungal protein from pumpkin rinds
RT   against various fungal pathogens.";
RL   J. Agric. Food Chem. 57:9299-9304(2009).
CC   -!- FUNCTION: Has strong antifungal activity against B.cinerea, C.albicans,
CC       C.coccodes, F.oxysporum, F.solani, T.harzianum and T.viride with EC(50)
CC       values of 20 uM, 20 uM, 10 uM, 10 uM, 10 uM, 20 uM and 20 uM,
CC       respectively. Has antifungal activity against A.fumigatus,
CC       A.parasiticus and D.byroniae with EC(50) values of 40 uM, 80 uM and 40
CC       uM, respectively. Lacks antifungal activity against P.verrucosum var.
CC       verrucosum. Lacks antibacterial activity against the Gram-negative
CC       bacterium E.coli and against the Gram-positive bacterium S.aureus. Has
CC       very low hemolytic activity against human erythrocytes. Increases
CC       fungal cell membrane permeability, probably by disrupting the membrane
CC       structure. {ECO:0000269|PubMed:19807165}.
CC   -!- MASS SPECTROMETRY: Mass=14865.57; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:19807165};
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DR   Proteomes; UP000504608; Unplaced.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antimicrobial; Cytolysis; Direct protein sequencing; Fungicide; Hemolysis;
KW   Plant defense; Reference proteome.
FT   CHAIN           1..>15
FT                   /note="Antifungal protein Pr-2"
FT                   /id="PRO_0000403319"
FT   NON_TER         15
FT                   /evidence="ECO:0000303|PubMed:19807165"
SQ   SEQUENCE   15 AA;  1557 MW;  1600E1BDC242A5F1 CRC64;
     QGIGVGDNDG KRGKR