ID   EGR2B_DANRE             Reviewed;         412 AA.
AC   Q05159;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Early growth response protein 2b;
DE            Short=EGR-2b;
DE   AltName: Full=Protein krx-20;
DE   AltName: Full=Zinc finger protein Krox-20;
GN   Name=egr2b; Synonyms=egr2, krx-20, krx20;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Embryo;
RX   PubMed=8464695; DOI=10.1093/nar/21.5.1087;
RA   Oxtoby E., Jowett T.;
RT   "Cloning of the zebrafish krox-20 gene (krx-20) and its expression during
RT   hindbrain development.";
RL   Nucleic Acids Res. 21:1087-1095(1993).
CC   -!- FUNCTION: Sequence-specific DNA-binding transcription factor. Binds to
CC       two specific DNA sites located in the promoter region of HOXA4.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- DEVELOPMENTAL STAGE: First detected during late gastrulation, found in
CC       the prospective neuroepithelium domain around 100% epiboly and begins
CC       to constrict along the neuroaxis by the end of gastrulation - around 10
CC       hours post-fertilization (hpf). By 12 hpf expression is restricted in
CC       two regions of the neuroepithelium of the prospective hindbrain;
CC       rhombomeres 3 and 5. Around 14 hpf neural crest migration begins from
CC       the dorsal surface of R5 moving caudally into R6 and then ventrally
CC       towards the pharyngeal arches. Crest migration is not apparent at or
CC       after 16 hpf and no migration is seen from R3. Expression is down-
CC       regulated first in R3 around 26 hpf and later in R5 around 30 hpf.
CC   -!- SIMILARITY: Belongs to the EGR C2H2-type zinc-finger protein family.
CC       {ECO:0000305}.
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DR   EMBL; X70322; CAA49790.1; -; mRNA.
DR   PIR; S30299; S30299.
DR   AlphaFoldDB; Q05159; -.
DR   STRING; 7955.ENSDARP00000062854; -.
DR   PaxDb; Q05159; -.
DR   PRIDE; Q05159; -.
DR   ZFIN; ZDB-GENE-980526-283; egr2b.
DR   eggNOG; KOG1721; Eukaryota.
DR   InParanoid; Q05159; -.
DR   Reactome; R-DRE-9031628; NGF-stimulated transcription.
DR   PRO; PR:Q05159; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0048568; P:embryonic organ development; IMP:ZFIN.
DR   GO; GO:0021575; P:hindbrain morphogenesis; IMP:ZFIN.
DR   GO; GO:0048932; P:myelination of posterior lateral line nerve axons; IMP:ZFIN.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0021654; P:rhombomere boundary formation; IMP:ZFIN.
DR   InterPro; IPR021849; EGR_N.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   Pfam; PF11928; DUF3446; 1.
DR   Pfam; PF00096; zf-C2H2; 3.
DR   SMART; SM00355; ZnF_C2H2; 3.
DR   SUPFAM; SSF57667; SSF57667; 2.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE   2: Evidence at transcript level;
KW   Activator; DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..412
FT                   /note="Early growth response protein 2b"
FT                   /id="PRO_0000047124"
FT   ZN_FING         299..323
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         329..351
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         357..379
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          269..299
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          371..412
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        387..412
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   412 AA;  45153 MW;  8DC42A9A0A400291 CRC64;
     MTAKTLEKAP VSLGGFVHPL ADSIYSVDEL GTTLPASVTI YNDLGGHYEQ INAGDGLING
     DMSTEKRALD LAYSSTFAQP AGPRNQTFTY MGKFSIDSQY PGNWNPEGVI NIVSAGILGM
     TQPSSASSSP ASSVSPSHFS STLSCTMAQN QADMEHIYSP PPPYSGCGEV YQDPSAFLST
     STCPISYPPP SYSSPKPNAD SGLFPIIPDY AGFFQPPCQR DMQSMPDRKP FSCPLDSFKL
     PPPLTPLNTI RNFTLGGPGP DGPRLPTAYT PQNLPLRPIL RPRKYPNRPS KTPVHERPYP
     CPAEGCDRRF SRSDELTRHI RIHTGHKPFQ CRICMRNFSR SDHLTTHIRT HTGEKPFACD
     FCGRKFARSD ERKRHTKIHL RQKERKSSSS STGVSSSERG VATSICSSSS NQ