ID   AFP_ENGPE               Reviewed;          18 AA.
AC   Q10722;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=30 kDa antifungal protein;
DE   Flags: Fragment;
OS   Engelmannia peristenia (Engelmann's daisy) (Engelmannia pinnatifida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC   Heliantheae alliance; Heliantheae; Engelmannia.
OX   NCBI_TaxID=53580;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, RESISTANCE TO HEAT AND PROTEOLYSIS, TISSUE
RP   SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC   TISSUE=Leaf {ECO:0000269|PubMed:8670144};
RX   PubMed=8670144; DOI=10.1042/bj3160723;
RA   Huynh Q.K., Borgmeyer J.R., Smith C.E., Bell L.D., Shah D.M.;
RT   "Isolation and characterization of a 30 kDa protein with antifungal
RT   activity from leaves of Engelmannia pinnatifida.";
RL   Biochem. J. 316:723-727(1996).
CC   -!- FUNCTION: Has antifungal activity against the plant fungal pathogens
CC       G.graminis, P.infestans, P.megasperma and S.sclerotiorum. Does not have
CC       antifungal activity against the human pathogen C.albicans. Does not
CC       have ribonuclease activity. {ECO:0000269|PubMed:8670144}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Temperature dependence:
CC         Thermostable. {ECO:0000269|PubMed:8670144};
CC   -!- TISSUE SPECIFICITY: Leaves. {ECO:0000269|PubMed:8670144}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in young plants, no expression in mature
CC       plants. {ECO:0000269|PubMed:8670144}.
CC   -!- MISCELLANEOUS: Resistant to heat and proteolysis.
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DR   PIR; S70611; S70611.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Fungicide.
FT   CHAIN           1..>18
FT                   /note="30 kDa antifungal protein"
FT                   /id="PRO_0000298773"
FT   NON_TER         18
FT                   /evidence="ECO:0000303|PubMed:8670144"
SQ   SEQUENCE   18 AA;  2091 MW;  A1F38FCFF0F451CA CRC64;
     XXTKFDFFTL ALQXPAXF