ID   EGR4_BOVIN              Reviewed;         482 AA.
AC   Q9GL32; Q9GL31;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Early growth response protein 4;
DE            Short=EGR-4;
GN   Name=EGR4;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], NUCLEOTIDE SEQUENCE [MRNA] OF 1-440,
RP   VARIANT PHE-322, ALTERNATIVE SPLICING, AND TISSUE SPECIFICITY.
RX   PubMed=12648095; DOI=10.1046/j.1365-2052.2003.00969.x;
RA   Nissen P.H., Thomsen B., Offenberg H., Thomsen P.D., Bendixen C.;
RT   "Cloning and genomic structure of the bovine EGR4 gene and evaluation as
RT   candidate gene for bovine spinal dysmyelination.";
RL   Anim. Genet. 34:124-131(2003).
CC   -!- FUNCTION: Transcriptional regulator. Recognizes and binds to the DNA
CC       sequence 5'-GCGGGGGCG-3' (GSG). Activates the transcription of target
CC       genes whose products are required for mitogenesis and differentiation
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9GL32-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9GL32-2; Sequence=VSP_022677;
CC   -!- TISSUE SPECIFICITY: Expressed in brain. In the cerebellum and frontal
CC       cortex. {ECO:0000269|PubMed:12648095}.
CC   -!- SIMILARITY: Belongs to the EGR C2H2-type zinc-finger protein family.
CC       {ECO:0000305}.
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DR   EMBL; AJ293899; CAC07207.1; -; Genomic_DNA.
DR   EMBL; AJ293900; CAC07208.2; -; mRNA.
DR   RefSeq; NP_001035587.1; NM_001040497.1. [Q9GL32-1]
DR   AlphaFoldDB; Q9GL32; -.
DR   SMR; Q9GL32; -.
DR   STRING; 9913.ENSBTAP00000033084; -.
DR   PaxDb; Q9GL32; -.
DR   PRIDE; Q9GL32; -.
DR   GeneID; 407155; -.
DR   KEGG; bta:407155; -.
DR   CTD; 1961; -.
DR   eggNOG; KOG1721; Eukaryota.
DR   InParanoid; Q9GL32; -.
DR   OrthoDB; 1318335at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   Pfam; PF00096; zf-C2H2; 3.
DR   SMART; SM00355; ZnF_C2H2; 3.
DR   SUPFAM; SSF57667; SSF57667; 2.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE   2: Evidence at transcript level;
KW   Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW   Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..482
FT                   /note="Early growth response protein 4"
FT                   /id="PRO_0000274191"
FT   ZN_FING         376..400
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         406..428
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         434..456
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          274..357
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        335..350
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         45
FT                   /note="A -> AA (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_022677"
FT   VARIANT         322
FT                   /note="L -> F"
FT                   /evidence="ECO:0000269|PubMed:12648095"
SQ   SEQUENCE   482 AA;  50304 MW;  51E4DB72410E4D61 CRC64;
     MLHLSEFSGP DALLVKSTEG CCSEPSTDLT RLPARDPPAA TGYPAGDFLS WALSSCGAGE
     YLTDSCLLEG PAPTPPPGLG YSGSFFIQAV PEHPHDPEAL FNLMSGILGL TPFPGAEAAA
     SRSPLDASFP AGSDALLPGP PDLFSPDPGA AAFPEAFWEA SPSAGAPSQC LYEPHLSAPD
     VKPGLRAPPA SPALDTASAF KGPYSPWELL SAGAPGSCGS QGGYQAAPEA RFPTTGAKIE
     DLLSISCPAE LPGGPASRLY TMGTYDAFPL APGDLGEGAE SLPGLLTPPS GEGGSSGEGG
     EFLDGTQPEL SPLGLRSATA DLPKPLVADI PGSSAVPEPP VPPPAPFPPA KARRKGRRGG
     KCSARCFCPR PHAKAFACPV ESCVRSFARS DELNRHLRIH TGHKPFQCRI CLRNFSRSDH
     LTTHVRTHTG EKPFACDVCG RRFARSDEKK RHSKVHLKQK ARAEERLKGL GFYSLGLSFA
     AL