EGR4_HUMAN
ID EGR4_HUMAN Reviewed; 589 AA.
AC Q05215; B2RAE3; G3V1T5; Q2Z1P5;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2013, sequence version 3.
DT 03-AUG-2022, entry version 183.
DE RecName: Full=Early growth response protein 4;
DE Short=EGR-4;
DE AltName: Full=AT133;
GN Name=EGR4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8504297; DOI=10.1093/hmg/2.4.367;
RA Holst C., Skerka C., Lichter P., Bialonski A., Zipfel P.F.;
RT "Genomic organization, chromosomal localization and promoter function of
RT the human zinc-finger gene pAT133.";
RL Hum. Mol. Genet. 2:367-372(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 49-589.
RX PubMed=1658795; DOI=10.1073/pnas.88.22.10079;
RA Mueller H.-J., Skerka C., Bialonski A., Zipfel P.F.;
RT "Clone pAT133 identifies a gene that encodes another human member of a
RT class of growth factor-induced genes with almost identical zinc-finger
RT domains.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:10079-10083(1991).
CC -!- FUNCTION: Transcriptional regulator. Recognizes and binds to the DNA
CC sequence 5'-GCGGGGGCG-3' (GSG). Activates the transcription of target
CC genes whose products are required for mitogenesis and differentiation
CC (By similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q05215; Q9NYB9-2: ABI2; NbExp=3; IntAct=EBI-19949420, EBI-11096309;
CC Q05215; O95817: BAG3; NbExp=3; IntAct=EBI-19949420, EBI-747185;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- INDUCTION: By PHA/PMA or by serum.
CC -!- SIMILARITY: Belongs to the EGR C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-104 is the initiator.
CC Some orthologous sequences cannot be extended. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAX88903.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAG36840.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA42698.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA49214.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X69438; CAA49214.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AK314154; BAG36840.1; ALT_INIT; mRNA.
DR EMBL; AC010913; AAX88903.1; ALT_INIT; Genomic_DNA.
DR EMBL; CH471053; EAW99734.1; -; Genomic_DNA.
DR EMBL; X60104; CAA42698.1; ALT_FRAME; mRNA.
DR PIR; A41537; A41537.
DR RefSeq; NP_001956.3; NM_001965.3.
DR AlphaFoldDB; Q05215; -.
DR SMR; Q05215; -.
DR BioGRID; 108281; 4.
DR IntAct; Q05215; 3.
DR STRING; 9606.ENSP00000445626; -.
DR iPTMnet; Q05215; -.
DR PhosphoSitePlus; Q05215; -.
DR BioMuta; EGR4; -.
DR DMDM; 485956294; -.
DR MassIVE; Q05215; -.
DR PaxDb; Q05215; -.
DR PeptideAtlas; Q05215; -.
DR PRIDE; Q05215; -.
DR ProteomicsDB; 32436; -.
DR ProteomicsDB; 58317; -.
DR Antibodypedia; 16536; 120 antibodies from 27 providers.
DR DNASU; 1961; -.
DR Ensembl; ENST00000545030.1; ENSP00000445626.1; ENSG00000135625.8.
DR GeneID; 1961; -.
DR KEGG; hsa:1961; -.
DR UCSC; uc010yrj.3; human.
DR CTD; 1961; -.
DR DisGeNET; 1961; -.
DR GeneCards; EGR4; -.
DR HGNC; HGNC:3241; EGR4.
DR HPA; ENSG00000135625; Tissue enriched (brain).
DR MIM; 128992; gene.
DR neXtProt; NX_Q05215; -.
DR OpenTargets; ENSG00000135625; -.
DR PharmGKB; PA27676; -.
DR VEuPathDB; HostDB:ENSG00000135625; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000162199; -.
DR InParanoid; Q05215; -.
DR OMA; LYKWGGC; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q05215; -.
DR TreeFam; TF318980; -.
DR PathwayCommons; Q05215; -.
DR Reactome; R-HSA-9031628; NGF-stimulated transcription.
DR SignaLink; Q05215; -.
DR BioGRID-ORCS; 1961; 21 hits in 1086 CRISPR screens.
DR GeneWiki; EGR4; -.
DR GenomeRNAi; 1961; -.
DR Pharos; Q05215; Tbio.
DR PRO; PR:Q05215; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q05215; protein.
DR Bgee; ENSG00000135625; Expressed in type B pancreatic cell and 87 other tissues.
DR ExpressionAtlas; Q05215; baseline and differential.
DR Genevisible; Q05215; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; NAS:ProtInc.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; TAS:ProtInc.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 2.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..589
FT /note="Early growth response protein 4"
FT /id="PRO_0000047128"
FT ZN_FING 483..507
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 513..535
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 541..563
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 29..101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 383..411
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 436..466
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 75..90
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 441..456
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 211
FT /note="I -> V (in Ref. 5; CAA42698 and 1; CAA49214)"
FT /evidence="ECO:0000305"
FT CONFLICT 291
FT /note="A -> R (in Ref. 5; CAA42698 and 1; CAA49214)"
FT /evidence="ECO:0000305"
FT CONFLICT 446
FT /note="V -> M (in Ref. 2; BAG36840)"
FT /evidence="ECO:0000305"
FT CONFLICT 530
FT /note="T -> S (in Ref. 1; CAA49214)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 589 AA; 61623 MW; EFB5A743541A07D7 CRC64;
MAVARGVGSP EPAPPQLYKW GGCGLGEPGS ALERRGAAAR GRCGRARAPR LPDSFPRGEC
PKPGARAPRS VRCGEPLPPA SPPPARPQAQ RARPRAPHSR RRAMLHLSEF SEPDALLVKS
TEGCCAEPSA ELPRLPARDA PAATGYPGAG DFLSWALNSC GASGDLADSC FLEGPAPTPP
PGLSYSGSFF IQAVPEHPHD PEALFNLMSG ILGLAPFPGP EAAASRSPLD APFPAGSDAL
LPGPPDLYSP DLGAAPFPEA FWEASPCAGA PSQCLYEPQL SPPDVKPGLR APPASPALDA
VSAFKGPYAP WELLSVGAPG NCGSQGDYQA APEARFPVIG TKIEDLLSIS CPAELPAVPA
NRLYPSGAYD AFPLAPGDLG EGAEGLPGLL TPPSGEGGSS GDGGEFLAST QPQLSPLGLR
SAAAADFPKP LVADIPGSSG VAAPPVPPPP PTPFPQAKAR RKGRRGGKCS TRCFCPRPHA
KAFACPVESC VRSFARSDEL NRHLRIHTGH KPFQCRICLR NFSRSDHLTT HVRTHTGEKP
FACDVCGRRF ARSDEKKRHS KVHLKQKARA EERLKGLGFY SLGLSFASL
