ID   AFP_PENRW               Reviewed;          92 AA.
AC   B6GXZ8;
DT   02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   16-DEC-2008, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Antifungal protein {ECO:0000250|UniProtKB:D0EXD3};
DE   Flags: Precursor;
GN   Name=afp {ECO:0000250|UniProtKB:D0EXD3}; ORFNames=Pc12g08290;
OS   Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin
OS   54-1255) (Penicillium chrysogenum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium;
OC   Penicillium chrysogenum species complex.
OX   NCBI_TaxID=500485;
RN   [1] {ECO:0000312|EMBL:CAP80456.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255;
RX   PubMed=18820685; DOI=10.1038/nbt.1498;
RA   van den Berg M.A., Albang R., Albermann K., Badger J.H., Daran J.-M.,
RA   Driessen A.J.M., Garcia-Estrada C., Fedorova N.D., Harris D.M.,
RA   Heijne W.H.M., Joardar V.S., Kiel J.A.K.W., Kovalchuk A., Martin J.F.,
RA   Nierman W.C., Nijland J.G., Pronk J.T., Roubos J.A., van der Klei I.J.,
RA   van Peij N.N.M.E., Veenhuis M., von Doehren H., Wagner C., Wortman J.R.,
RA   Bovenberg R.A.L.;
RT   "Genome sequencing and analysis of the filamentous fungus Penicillium
RT   chrysogenum.";
RL   Nat. Biotechnol. 26:1161-1168(2008).
CC   -!- FUNCTION: Strong antifungal activity against the molds P.commune Pc332,
CC       P.echinulatum Pe321, and A.niger An261. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:D0EXD3}.
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DR   EMBL; AM920427; CAP80456.1; -; Genomic_DNA.
DR   RefSeq; XP_002557660.1; XM_002557614.1.
DR   PDB; 7BAD; X-ray; 1.69 A; A=35-92.
DR   PDB; 7BAE; X-ray; 1.20 A; A=35-92.
DR   PDB; 7BAF; X-ray; 1.12 A; A=35-92.
DR   PDBsum; 7BAD; -.
DR   PDBsum; 7BAE; -.
DR   PDBsum; 7BAF; -.
DR   AlphaFoldDB; B6GXZ8; -.
DR   SMR; B6GXZ8; -.
DR   STRING; 500485.B6GXZ8; -.
DR   EnsemblFungi; CAP80456; CAP80456; PCH_Pc12g08290.
DR   GeneID; 8309088; -.
DR   KEGG; pcs:Pc12g08290; -.
DR   VEuPathDB; FungiDB:PCH_Pc12g08290; -.
DR   eggNOG; ENOG502RPIB; Eukaryota.
DR   HOGENOM; CLU_182418_0_0_1; -.
DR   OMA; ITRIAIF; -.
DR   OrthoDB; 1618692at2759; -.
DR   BioCyc; PCHR:PC12G08290-MON; -.
DR   Proteomes; UP000000724; Contig Pc00c12.
DR   GO; GO:0005576; C:extracellular region; ISS:UniProtKB.
DR   GO; GO:0050832; P:defense response to fungus; ISS:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.60; -; 1.
DR   InterPro; IPR023112; Antifungal-protein_dom_sf.
DR   InterPro; IPR022706; Antifungal_prot.
DR   Pfam; PF11402; Antifungal_prot; 1.
DR   SUPFAM; SSF57598; SSF57598; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antimicrobial; Disulfide bond; Fungicide; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   PROPEP          19..34
FT                   /evidence="ECO:0000250|UniProtKB:D0EXD3"
FT                   /id="PRO_0000391703"
FT   CHAIN           35..92
FT                   /note="Antifungal protein"
FT                   /evidence="ECO:0000250|UniProtKB:D0EXD3"
FT                   /id="PRO_0000391704"
FT   DISULFID        42..70
FT                   /evidence="ECO:0000250|UniProtKB:P17737"
FT   DISULFID        49..77
FT                   /evidence="ECO:0000250|UniProtKB:P17737"
FT   DISULFID        62..88
FT                   /evidence="ECO:0000250|UniProtKB:P17737"
FT   STRAND          37..43
FT                   /evidence="ECO:0007829|PDB:7BAF"
FT   TURN            44..47
FT                   /evidence="ECO:0007829|PDB:7BAF"
FT   STRAND          48..53
FT                   /evidence="ECO:0007829|PDB:7BAF"
FT   STRAND          56..61
FT                   /evidence="ECO:0007829|PDB:7BAF"
FT   TURN            66..68
FT                   /evidence="ECO:0007829|PDB:7BAF"
FT   STRAND          76..80
FT                   /evidence="ECO:0007829|PDB:7BAF"
FT   TURN            81..84
FT                   /evidence="ECO:0007829|PDB:7BAF"
FT   STRAND          85..88
FT                   /evidence="ECO:0007829|PDB:7BAF"
SQ   SEQUENCE   92 AA;  10046 MW;  5CCE4175B6E03BE2 CRC64;
     MHITSIAIVF FAAMGAVASP IATESDDLDA RDVQLSKFGG ECSLKHNTCT YLKGGKNHVV
     NCGSAANKKC KSDRHHCEYD EHHKRVDCQT PV