ID   EGT_NPVAC               Reviewed;         506 AA.
AC   P18569;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   23-FEB-2022, entry version 74.
DE   RecName: Full=Ecdysteroid UDP-glucosyltransferase;
DE            EC=2.4.1.-;
DE   Flags: Precursor;
GN   Name=EGT; Synonyms=UGT21A1;
OS   Autographa californica nuclear polyhedrosis virus (AcMNPV).
OC   Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX   NCBI_TaxID=46015;
OH   NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=L1;
RX   PubMed=2505387; DOI=10.1126/science.2505387;
RA   O'Reilly D.R., Miller L.K.;
RT   "A baculovirus blocks insect molting by producing ecdysteroid UDP-glucosyl
RT   transferase.";
RL   Science 245:1110-1112(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=L1;
RX   PubMed=2106039; DOI=10.1128/jvi.64.3.1321-1328.1990;
RA   O'Reilly D.R., Miller L.K.;
RT   "Regulation of expression of a baculovirus ecdysteroid
RT   UDPglucosyltransferase gene.";
RL   J. Virol. 64:1321-1328(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C6;
RX   PubMed=8030224; DOI=10.1006/viro.1994.1380;
RA   Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.;
RT   "The complete DNA sequence of Autographa californica nuclear polyhedrosis
RT   virus.";
RL   Virology 202:586-605(1994).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-87.
RC   STRAIN=L1;
RX   PubMed=8497062; DOI=10.1128/jvi.67.6.3481-3488.1993;
RA   Passarelli A.L., Miller L.K.;
RT   "Identification and characterization of lef-1, a baculovirus gene involved
RT   in late and very late gene expression.";
RL   J. Virol. 67:3481-3488(1993).
RN   [5]
RP   FUNCTION, AND GLYCOSYLATION.
RX   PubMed=10073711; DOI=10.1099/0022-1317-80-2-485;
RA   Evans O.P., O'Reilly D.R.;
RT   "Expression and structural characterization of a baculovirus ecdysteroid
RT   UDP-glucosyltransferase.";
RL   J. Gen. Virol. 80:485-492(1999).
CC   -!- FUNCTION: Catalyzes the transfer of glucose from UDP-glucose to
CC       ecdysteroids which are insect molting hormones. Acts on the host at the
CC       organismal level to block its development, thereby increasing the yield
CC       of progeny virus. {ECO:0000269|PubMed:10073711}.
CC   -!- PTM: Glycosylated. {ECO:0000269|PubMed:10073711}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; M22619; AAA69845.1; -; Genomic_DNA.
DR   EMBL; L09723; AAA46712.1; -; Genomic_DNA.
DR   EMBL; M96361; AAA66785.1; -; Genomic_DNA.
DR   EMBL; L22858; AAA66645.1; -; Genomic_DNA.
DR   PIR; A34114; XUNVAC.
DR   RefSeq; NP_054044.1; NC_001623.1.
DR   SMR; P18569; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   GeneID; 1403847; -.
DR   KEGG; vg:1403847; -.
DR   Proteomes; UP000008292; Genome.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR016224; Ecdysteroid_UDP-Glc_Trfase.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   Pfam; PF00201; UDPGT; 1.
DR   PIRSF; PIRSF000476; Ecdystd_UDP_glucosyltfrase; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Glycoprotein; Glycosyltransferase; Reference proteome; Signal; Transferase.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..506
FT                   /note="Ecdysteroid UDP-glucosyltransferase"
FT                   /id="PRO_0000036057"
SQ   SEQUENCE   506 AA;  57033 MW;  C83FA34D2453A606 CRC64;
     MTILCWLALL STLTAVNAAN ILAVFPTPAY SHHIVYKVYI EALAEKCHNV TVVKPKLFAY
     STKTYCGNIT EINADMSVEQ YKKLVANSAM FRKRGVVSDT DTVTAANYLG LIEMFKDQFD
     NINVRNLIAN NQTFDLVVVE AFADYALVFG HLYDPAPVIQ IAPGYGLAEN FDTVGAVARH
     PVHHPNIWRS NFDDTEANVM TEMRLYKEFK ILANMSNALL KQQFGPNTPT IEKLRNKVQL
     LLLNLHPIFD NNRPVPPSVQ YLGGGIHLVK SAPLTKLSPV INAQMNKSKS GTIYVSFGSS
     IDTKSFANEF LYMLINTFKT LDNYTILWKI DDEVVKNITL PANVITQNWF NQRAVLRHKK
     MAAFITQGGL QSSDEALEAG IPMVCLPMMG DQFYHAHKLQ QLGVARALDT VTVSSDQLLV
     AINDVLFNAP TYKKHMAELY ALINHDKATF PPLDKAIKFT ERVIRYRHDI SRQLYSLKTT
     AANVPYSNYY MYKSVFSIVM NHLTHF