EGY1_ORYSJ
ID EGY1_ORYSJ Reviewed; 579 AA.
AC Q852K0; Q0DMV1;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 3.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Probable zinc metalloprotease EGY1, chloroplastic;
DE EC=3.4.24.-;
DE AltName: Full=Protein ETHYLENE-DEPENDENT GRAVITROPISM-DEFICIENT AND YELLOW-GREEN 1;
DE Short=OsEGY1;
DE Flags: Precursor;
GN Name=EGY1; OrderedLocusNames=Os03g0792400, LOC_Os03g57840;
GN ORFNames=OSJNBb0060J21.23;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP GENE FAMILY.
RX PubMed=15659096; DOI=10.1111/j.1365-313x.2004.02308.x;
RA Chen G., Bi Y.R., Li N.;
RT "EGY1 encodes a membrane-associated and ATP-independent metalloprotease
RT that is required for chloroplast development.";
RL Plant J. 41:364-375(2005).
CC -!- FUNCTION: Probable membrane-associated metalloprotease that may be
CC involved in chloroplast development. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q852K0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q852K0-2; Sequence=VSP_054026;
CC -!- SIMILARITY: Belongs to the peptidase M50B family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF13437.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC090871; AAO37991.2; -; Genomic_DNA.
DR EMBL; DP000009; ABF99299.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF99300.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF13437.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014959; BAS86794.1; -; Genomic_DNA.
DR EMBL; AK064808; BAG89215.1; -; mRNA.
DR RefSeq; XP_015630593.1; XM_015775107.1. [Q852K0-1]
DR AlphaFoldDB; Q852K0; -.
DR STRING; 4530.OS03T0792400-01; -.
DR PaxDb; Q852K0; -.
DR PRIDE; Q852K0; -.
DR EnsemblPlants; Os03t0792400-01; Os03t0792400-01; Os03g0792400. [Q852K0-2]
DR GeneID; 4334387; -.
DR Gramene; Os03t0792400-01; Os03t0792400-01; Os03g0792400. [Q852K0-2]
DR KEGG; osa:4334387; -.
DR eggNOG; ENOG502QUAP; Eukaryota.
DR HOGENOM; CLU_028221_1_1_1; -.
DR InParanoid; Q852K0; -.
DR OMA; NKYNLFM; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q852K0; OS.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR InterPro; IPR044838; EGY1-like.
DR PANTHER; PTHR31412; PTHR31412; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Hydrolase; Membrane; Metalloprotease;
KW Plastid; Protease; Reference proteome; Transit peptide; Transmembrane;
KW Transmembrane helix.
FT TRANSIT 1..44
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 45..579
FT /note="Probable zinc metalloprotease EGY1, chloroplastic"
FT /id="PRO_0000428646"
FT TRANSMEM 272..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 419..439
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 452..472
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 505..525
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 547..567
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 78..146
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 124..146
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..117
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_054026"
SQ SEQUENCE 579 AA; 61155 MW; B80A595AAB17336C CRC64;
MAAAAAALAS SPMVHLTASR LRLPRPARSP AAATPSPSPA SAACCSRGAA CGLEWRPKSG
LRALRRCEDR LRCFSIDGGG GGGGGGGGGT GGEDGEKRGE EEAAAAAEAK VGGAVEEMRS
ERTRSGSFSS SSSSSSGTPG ISNEPPFLSF SVDNIDTVKL LELLGPEKVD SADVKAIKEK
LFGYTTFWLT REEPFGDLGE GVLFIGNLRG KREEIFAKLQ QQLRELTGDK YNLFMVEEPN
SEGEDPRGGP RVSFGLLRRE VSEPGPTTLW QYVISLLLFL LTVFSCVELG IASKISSLPP
EIVTYFTDPN ATGPPPDMQL LLPFVESALP VAYGVLAIQL FHEVGHFLAA FPKKVKLSIP
FFIPNFTLGT FGAITQFKSI LPDKKTMFDI SMAGPLAGAA LSFSMFSVGL LLSSNPAGAS
DLVEVPSKLF QGSLLLGLVS RATLGYRAMH AATVAIHPLV IAGWCGLTTT AFNMLPVGCL
DGGRALQGAF GKDALFGFGL TTYSLLGLGV LGGPLSLPWG LYVLICQRTP EKPCLNDVSD
VGTWRRAALI VSVFLVVLTL IPLWDELAED LGVGLVTSF
