EGY2_ARATH
ID EGY2_ARATH Reviewed; 556 AA.
AC Q9FFK3;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Probable zinc metalloprotease EGY2, chloroplastic;
DE EC=3.4.24.-;
DE AltName: Full=Protein ETHYLENE-DEPENDENT GRAVITROPISM-DEFICIENT AND YELLOW-GREEN 2;
DE Flags: Precursor;
GN Name=EGY2; OrderedLocusNames=At5g05740; ORFNames=MJJ3.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=15659096; DOI=10.1111/j.1365-313x.2004.02308.x;
RA Chen G., Bi Y.R., Li N.;
RT "EGY1 encodes a membrane-associated and ATP-independent metalloprotease
RT that is required for chloroplast development.";
RL Plant J. 41:364-375(2005).
CC -!- FUNCTION: Probable membrane-associated metalloprotease that may be
CC involved in chloroplast development. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9FFK3-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the peptidase M50B family. {ECO:0000305}.
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DR EMBL; AB005237; BAB09668.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90918.1; -; Genomic_DNA.
DR RefSeq; NP_196193.1; NM_120656.3. [Q9FFK3-1]
DR AlphaFoldDB; Q9FFK3; -.
DR STRING; 3702.AT5G05740.1; -.
DR MEROPS; M50.A01; -.
DR PaxDb; Q9FFK3; -.
DR PRIDE; Q9FFK3; -.
DR ProteomicsDB; 220756; -. [Q9FFK3-1]
DR EnsemblPlants; AT5G05740.1; AT5G05740.1; AT5G05740. [Q9FFK3-1]
DR GeneID; 830458; -.
DR Gramene; AT5G05740.1; AT5G05740.1; AT5G05740. [Q9FFK3-1]
DR KEGG; ath:AT5G05740; -.
DR Araport; AT5G05740; -.
DR TAIR; locus:2166389; AT5G05740.
DR eggNOG; ENOG502QVZT; Eukaryota.
DR InParanoid; Q9FFK3; -.
DR OrthoDB; 1393607at2759; -.
DR PhylomeDB; Q9FFK3; -.
DR PRO; PR:Q9FFK3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FFK3; baseline and differential.
DR Genevisible; Q9FFK3; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR InterPro; IPR044838; EGY1-like.
DR PANTHER; PTHR31412; PTHR31412; 1.
DR PROSITE; PS00142; ZINC_PROTEASE; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Chloroplast; Hydrolase; Membrane; Metalloprotease;
KW Plastid; Protease; Reference proteome; Transit peptide; Transmembrane;
KW Transmembrane helix.
FT TRANSIT 1..64
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 65..556
FT /note="Probable zinc metalloprotease EGY2, chloroplastic"
FT /id="PRO_0000428647"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 437..457
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 484..504
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 527..547
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 63..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 77..91
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 93..129
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 556 AA; 60064 MW; C41CFFBB72E9C585 CRC64;
MNLAVASFRG NFGVLSQCSS CCSLQFQPFV AATSSLNFGQ TGTSRRKKDL KLERVFRKRE
TLVRVTETQT EPEGNDDEDN KEGKESSADD PPTKIPTELN SQSTVVNEAP GNEEENKAQF
SSQDGDKLEV SSGSPLPGVN VSIIIHVIYK DDSIMFSGCL SFIKSCCEQP LQLDDSMRLP
KETIDILRGQ VFGFDTFFVT SQEPYEGGVL FKGNLRGKPA TSYEKIKTRM ENNFGDQYKL
FLLTNPEDDK PVAVVVPRRS LEPETTAVPE WFAAGSFGLV ALFTLFLRNV PALQSDLLSA
FDNLELLKDG LPGALVTALV LGVHELGHIL VANSLGIKLG VPFFVPSWQI GSFGAITRIK
NIVAKREDLL KVAAAGPLAG FSLGLILFLI GLFVPPSDGI GVVVDASVFH ESFLAGGIAK
LLLGDALKEG TSISLNPLVI WAWAGLLING INSIPAGELD GGKIAFSIWG RKTATRLTGA
SIALLGLSAL FSDVAFYWVV LIFFLQRGPI APLAEEITVP DDKYVSLGIL VLFLSLLVCL
PYPFAFTGNE AMMIGL
