ID   EGY2_ORYSI              Reviewed;         545 AA.
AC   B8AD72;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Probable zinc metalloprotease EGY2, chloroplastic;
DE            EC=3.4.24.-;
DE   AltName: Full=Protein ETHYLENE-DEPENDENT GRAVITROPISM-DEFICIENT AND YELLOW-GREEN 2;
DE   Flags: Precursor;
GN   Name=EGY2; ORFNames=OsI_00340;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- FUNCTION: Probable membrane-associated metalloprotease that may be
CC       involved in chloroplast development. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC       Multi-pass membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase M50B family. {ECO:0000305}.
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DR   EMBL; CM000126; EEC69919.1; -; Genomic_DNA.
DR   AlphaFoldDB; B8AD72; -.
DR   STRING; 39946.B8AD72; -.
DR   EnsemblPlants; BGIOSGA002422-TA; BGIOSGA002422-PA; BGIOSGA002422.
DR   Gramene; BGIOSGA002422-TA; BGIOSGA002422-PA; BGIOSGA002422.
DR   HOGENOM; CLU_030692_0_0_1; -.
DR   OMA; GWGFYRA; -.
DR   Proteomes; UP000007015; Chromosome 1.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR044838; EGY1-like.
DR   PANTHER; PTHR31412; PTHR31412; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   3: Inferred from homology;
KW   Chloroplast; Hydrolase; Membrane; Metalloprotease; Plastid; Protease;
KW   Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT   TRANSIT         1..63
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           64..545
FT                   /note="Probable zinc metalloprotease EGY2, chloroplastic"
FT                   /id="PRO_0000428649"
FT   TRANSMEM        256..276
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        300..320
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        325..345
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        363..383
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        426..446
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        473..493
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        513..533
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          66..142
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        89..106
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        113..136
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   545 AA;  58667 MW;  20E4041A444BE1C7 CRC64;
     MQLPAMSCSP SQSSAAAAAA YGCCQRILLA STSLPATGRP ARLGLKLRST HSLQIRNRRF
     VCQAMTETEP DGDGNGDEEK EELGDDASSP SVYSVTQENG SAESETNADN TKDETVNTEP
     LSSSDTVQNI DGDATPASDA QENVEVVDVA VGSPLPGMKQ QLDESVRIPK ATIDILKDQV
     FGFDTFFVTS QEPYEGGILF KGNLRGQPAK SYEKITNRLQ NKFGDQYKLF LLINPEDDKP
     VAVVVPRQTL QPETTAVPEW FAAASFGVVT IFTLLLRNVP LLQDNLLSTF DNLELLKDGV
     YGALVTAAII GVHEIAHILA ARDTGIKLAV PYFVPSWQIG SFGAITRIVN IVRNREDLLK
     VAAAGPLAGF SLGFVLLLLG FILPPSDGLG LVIDPAVFHE SFLVGGLAKL ILGDALKEGT
     KLSINPLVLW AWAGLLINAI NSIPAGELDG GRIAFAMWGR KISSRISSLA IGLLGISALF
     NDVAFYWVVL IFFLQRGPIS PLSEEITEPE NNYISIGVAI LLFGLLVCLP YPFPFDPSQL
     TDFDL